Browsing by Subject "Supplements"

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    Maternal non-compliance with recommended folic acid supplement use alters global DNA methylation in cord blood of newborns: a cohort study
    (Elsevier, 2024) Morales Bartolomé, Eva; Prieto Sánchez, María Teresa; Mendiola Olivares, Jaime; Cutillas Tolín, Ana; Adoamnei, Evdochia; Valera-Gran, Desiree; Santaella-Pascual, Marina; Suárez Martínez, Clara; Vioque, Jesús; Castaños, María Jesús; Castillo, Eva del; García-Marcos Álvarez, Luis Vicente; Tecnología de Alimentos, Nutrición y Bromatología; Facultad de Veterinaria
    Background & aims: Prenatal folate exposure may alter epigenetic marks in the offspring. We aimed to evaluate associations between prenatal exposure to folic acid (FA) in preconception and in utero with cord blood DNA methylation in long interspersed nuclear element 1 (LINE-1) and Alu short interspersed nuclear elements (SINEs) as markers of global DNA methylation levels. Methods: Data come from 325 motherechild pairs participating in the Nutrition in Early Life and Asthma (NELA) birth cohort (2015e2018). Pregnant women were asked about supplement use, including brand name and dose, one month before pregnancy (preconception) and through the trimesters of pregnancy. Maternal dietary folate intake was assessed using a validated food frequency questionnaire with additional questions for FA supplement use. Folate serum levels were measured in mothers at 24 weeks of gestation and in cord blood of newborns. DNA methylation was quantitatively assessed by bisulfite pyrosequencing on 5 LINE-1 and 3 Alu different elements. Associations were estimated using multivariable linear regression models. Results: A reduction in methylation levels of LINE-1 in newborns was associated with the use of FA supplements below the recommended doses (<400 ug/day) during preconception ( 0.50; 95% CI:  0.91,  0.09; P ¼ 0.016), and from preconception up to 12 weeks of gestation ( 0.48; 95% CI:  0.88,  0.08; P ¼ 0.018). Maternal use of FA supplements above the tolerable upper intake level of 1000 ug/day from preconception until 12 weeks of gestation was also related to lower methylation in LINE-1 at birth ( 0.77; 95% CI:  1.52,  0.02; P ¼ 0.044). Neither FA supplement use after 12 weeks of gestation nor maternal total folate intake (diet plus supplements) were associated with global DNA methylation levels at birth.