Browsing by Subject "Sublingual gland"

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    Effects of estrogen on STIM1/Orai1 in the sublingual gland of ovariectomized rats
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2020) Bai, Yun; Li, Bing; Wang, Sinan; Jiang, Hai; Li, Junlei; Wang, Wenjuan; Wang, Ke; Qin, Lihua; Jia, Jing
    Background. Studies have shown that estrogen can protect the function of the sublingual gland, but the specific mechanism is still unclear. Besides, the STIM1/Orai1 pathway is important to secretion in the salivary gland. Here, we explore the possible effects of estrogen on sublingual gland function by observing changes of STIM1 and Orai1 levels in the sublingual glands of ovariectomized rats. Methods. 42 adult female Sprague-Dawley rats were randomly divided into three groups: SHAM, OVX, and OVX+E (n=14 per group). Two weeks after ovariectomy, rats were treated with estrogen (β-estradiol). The expression of STIM1 and Orai1 in the sublingual gland were observed by double label-immunohistochemistry and immunofluorescence. Calcium imaging was conducted to observe changes in cellular Ca 2+ levels. Results. IHC and IF showed that the levels of both STIM1 and Orai1 decreased following ovariectomy, but increased to SHAM levels after estrogen treatment. By IF, STIM1 and Orai1 exhibited perfect co-localization. Calcium imaging results showed that the Ca 2+ in the cells decreased after ovariectomy. Estrogen intervention returned levels of these proteins and Ca 2+ to the same as those in the control group. Conclusion. This study demonstrates that low estrogen status significantly reduced the expression of STIM1 and Orai1 in the sublingual gland of rats, along with cellular Ca 2+ levels. These data provide insight into the likely mechanisms underlying sublingual gland secretion dysfunction during menopause
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    Variety of sialic acids occurring in the bovine sublingual gland
    (Murcia : F. Hernández, 1994) Accili, Daniela; Gabrielli, M.G.; Menghi, Giovanna
    Sialoglycoconjugates were investigated in the bovine sublingual gland by direct visualization of sialic acid with specific lectins (LPA, SNA) and by histochemical procedures combined with sialidase digestion and lectins. The most reactive histological sructures were found to be acini which contained glycoconjugates with terminal disaccharides consisting of sialic acid linked to galactose or N-acetylgalactosamine. Resistance to periodate oxidation was interpreted as demonstrating a relevant presence of C7, C8 and Cg acetylated sialic acids. KOH-Sialidase-DBA and KOHAlcian blue sequences allowed the identification of C4 acetylated sialic acids.