Browsing by Subject "Streptozotocin"

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    Antidiabetic effect of Atriplex halimus L. (Sp. Pl. 2: 1052 (1753)) long and short term treatment against streptozotocin induced diabetes in rats
    (Universidad de Murcia. Servicio de publicaciones, 2022) Bounouar, Elaid; Missoun, Fatiha; Ouda Amari, Nesrine; Zohra Belabaci, Fatima; Belabaci, Senia; Zohra Sekkal, Fatima; Djebli, Noureddine
    El objetivo fue evaluar la actividad antidiabética de hojas de Atriplex halimus en ratas diabéticas modelo. Para evaluar se utilizó la glicemia, pérdida de peso, volumen de consumo de agua, parámetros bioquímicos. estudio histológico. Las investigaciones fitoquímicas indican la presencia de flavonoides, taninos, saponinas, mucílagos, glucósidos. proteínas. Los resultados muestran que el tratamiento con extracto acuoso de A. halimus presenta reducción significativa de los niveles de glucosa en sangre en ratas de los grupos D100. D200, en comparación con el grupo diabético,. protege. las ratas de complicaciones diabéticas. El estudio histológico del páncreas lo confirman por la mejora en los islotes de Langerhans de rata tratada con este extracto vegetal. Atriplex halimus parece ser una planta prometedora para futuros ensayos preclínicos. clínicos en la diabetes de tipo I.
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    Experimental diabetes modulates collagen remodelling of joints in rats
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 2012) Atayde, Sandra A.; Yoshinari, Natalino H.; Nascimento, Dafne P.; Catanozi, Sérgio; Andrade, Priscila C.; Velosa, Ana Paula P.; R. Parra, Edwin; Passarelli, Marisa; Nakandakare, Edna R.; Capelozzi, Vera L.; Teodoro, Walcy R.
    The aim of this study was to evaluate extracellular matrix components in articular cartilage, ligaments and synovia in an experimental model of diabetes. Young Wistar rats were divided into a streptozotocin-induced (STZ; 35 mg/kg) diabetic group (DG; n=15) and a control group (CG; n=15). Weight, blood glucose and plasma anti-carboxymethyllysine were measured 70 days after STZ infusions. Knee joints, patellar ligaments, and lateral and medial collateral ligaments were isolated and stained with hematoxylin-eosin and Picrosirius. The total collagen content was determined by morphometry. Immunofluorescence was employed to evaluate types I, III, and V collagen in ligaments and synovial tissues and types II and XI collagen in cartilage. Results: Higher blood glucose levels and plasma anti-carboxymethyllysine were observed in DG rats when compared to those in CG rats. The final weight was significantly lower in the DG rats than in the CG rats. Histomorphometric evaluation depicted a small quantity of collagen fibers in ligaments and articular cartilage in DG rats, as well as increased collagen in synovial tissue. There was a decrease in cartilage proteoglycans in DG rats when compared with CG rats. Immunofluorescence staining revealed an increase of collagen III and V in ligaments, collagen XI in cartilage, and collagen I in synovial tissue of DG rats compared with CG rats. Conclusion: The ligaments, cartilage and synovia are highly affected following STZ-induced diabetes in rats, due the remodeling of collagen types in these tissues. This process may promote the degradation of the extracellular matrix, thus compromising joint function. Our data may help to better understand the pathogenesis of joint involvement related to diabetes.
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    Morphometric study on the renal glomeruli of streptozotocin (SZ)-induced diabetic APA hamsters
    (Murcia : F. Hernández, 1992) Han, J.S.; Doi, K.
    Morphometrical analysis was done on the renal glomeruli of streptozotocin (SZ)-induced diabetic and control APA hamsters. In coincidence with the histopathological and ultrastructural findings, the areas of whole glomerulus (WG) and mesangial region (MR) were significantly larger in diabetic animals than in controls at 1 and 3 months after SZ-injection (1 and 3MAI). The area of capillary lumen in diabetic animals was larger than that in controls at lMAI but it became similar between both groups at 3MAI probably due to an increase in the area of MR. The thickness of basement membrane was significantly larger in diabetic animals than in controls at 3MAI. The present morphometrical findings, together with histological and ultrastructural ones, suggest that SZ-induced diabetic APA hamsters are useful as a model system for the investigation of focal and segmental glomerulosclerosis.