Browsing by Subject "Stem Cells"

Now showing 1 - 3 of 3
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    Depletion of SHANK2 inhibited the osteo/dentinogenic differentiation potentials of stem cells from apical papilla
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Guo, Lijia; Jin, Luyuan; Du, Juan; Zhang, Chunmei; Fan, Zhipeng; Wang, Songlin
    The aim of this study was to investigate the biological function of SHANK2 on the osteo/ dentinogenic differentiation potentials of human stem cells from apical papilla (SCAPs). Real-time RT-PCR was used to detect the expression of SHANK2 in human mesenchymal stem cells (MSCs). Small hairpin RNA (shRNA) was used to knockdown the SHANK2 in SCAPs. The knockdown efficiency was determined by real-time RT-PCR and Western Blot. The in vitro osteo/dentinogenic differentiation potentials of SCAPs were investigated using ALP staining, ALP activity, alizarin red staining, quantitative calcium, the expression levels of DSPP, DMP1, RUNX2 and OSX. In vivo transplantation experiments in immunocompromised mice were used to evaluate the capacity of SCAPs to form bone/dentine-like structure. SHANK2 was highly expressed in dental tissue-derived MSCs compared with cells of other origins. Silencing of SHANK2 inhibited the ALP activity, mineralization, and the expressions of DSPP, DMP1, RUNX2 and OSX in SCAPs. Furthermore, in vivo transplantation experiments indicated that knockdown of SHANK2 in SCAPs generated less bone/dentinlike mineralized tissue compared with the control group. The present study demonstrated that depletion of SHANK2 inhibited the osteo/dentinogenic differentiation potentials in SCAPs, explored the new function of SHANK2, and provided useful information to elucidate the molecular mechanism underlying directed differentiation in dental tissue-derived MSCs.
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    Medicina regenerativa en el cuidado de las úlceras del pie diabético. Una Revisión Sistemática
    (Universidad de Murcia: servicio de publicaciones, 2025) Conrado Jorge-Finnigan; Marta Celorrio San Miguel; Saray Rodríguez-García; Ana Belén Dueñas-Pérez; Elena Jiménez-Callejo; Diego Fernández Lázaro; Sin departamento asociado
    ntroduction:The pluripotency of stem cells (SC) and platelet-rich plasma (PRP) makes them potential candidates to accelerate tissue repair processes in lesions such as diabetic foot ulcers (DFU). Objective: Our objective was to evaluate the current evidence on the benefits of healing DFUs that do not respond to conventional treatment with SC and/or PRP. Method:Based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA), we systematically review original studies published in the last 5 years and indexed in Web of Science, Scopus, CUIDEN, and PubMed to evaluate the effects of SC and/or PRP on skin markers, healing time and adverse effects in DFU. The study was registered in PROSPERO (CRD 42024537847). Results: Among 107 records identified in the search, 5 studies met the inclusion criteria. DFUs treated with topically administered PRP or intralesional injection achieved a significant (p < 0.05) reduction in ulcer/ wound area. The overall cure rate improved significantly (p < 0.05) after the application of SC. Treatment with SC was able to reduce the amputation rate non-significantly (p > 0.05). The epithelialization or healing processes did not experience significant changes (p > 0.05) after the use of PRP or SC. No serious adverse effects were reported. Conclusion:Cell therapy with SC and/or PRP on DFUs that do not heal with conventional treatment is a safe and effective therapeutic option
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    Microgravity directs stem cell differentiation
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Chen, Zhe; Luo, Qing; Yuan, Lin; Song, Guanbin
    y. Stem cells are the cell of origin for organisms and their organs. These cells are critical for tissue regeneration, as well as regenerative medicine. Mechanical forces, such as gravity, have been demonstrated to provide important signals for stem cell fate. In fact, the absence of gravity, that is, microgravity, affects almost all aspects of human physiology, which has been partly attributed to changes in the biological behaviors of stem cells. In this review, we summarize the current understanding of the effects of microgravity on stem cell differentiation that control the fate of stem cells.