Browsing by Subject "Sprouts"
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- PublicationOpen AccessBroccoli and Radish Sprouts are Safe and Rich in Bioactive Phytochemicals(Elsevier, ) Baenas, Nieves; Gómez-Jodar, Isabel; Moreno, Diego A.; García-Viguera, Cristina; Periago, Paula M.; Tecnología de Alimentos, Nutrición y BromatologíaCruciferous sprouts (e.g. broccoli and red radish) are rich source of health-promoting phytochemicals more concentrated than the adult plant edible organs; however, these tiny microgreens need cold storage conditions to preserve their quality to reach the consumers in microbiologically safe conditions, maintaining their composition and acceptability. In this work, the microbiological status and phytochemical composition of broccoli and radish sprouts were evaluated at harvest (Day 0), and after seven and fourteen days of storage at two different temperatures, 5 and 10 °C. Pathogenic microorganisms were absent during shelf-life; nevertheless, the slight growth of Enterobacteriaceae organisms, aerobic mesophilic and psychrotrophic bacteria, molds and yeasts was assessed. The temperature of storage highly influenced the quality and content of bioactives in the sprouts, and for practical applications, the storage at 5 ºC is the most suitable option. Moreover, these fresh crucifers remain acceptable for consumers after a long refrigerated storage period (14 d), being an interesting option for consuming fresh and naturally-functional foods.
- PublicationOpen AccessRadish sprouts : Characterization and elicitation of novel varieties rich in anthocyanins(Elsevier, 2015-01-16) Baenas, Nieves; Ferreres, Federico; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y BromatologíaThe anthocyanin profile of two varieties of red radish sprouts (Raphanus sativus), cv. China rose and Rambo, were studied using HPLC-DAD-ESI-MSn and HPLC-DAD. The most abundant type of anthocyanins was cyanidin and its derivatives, with one or two acylated groups, with qualitative and quantitative differences among varieties. Some compounds were identified for the first time in both varieties, as we are concern. Radish sprouts were treated during germination (day 3 to 8) using methyl jasmonate, jasmonic acid, salicylic acid, sucrose and glucose as elicitors in order to enrich their total anthocyanins content (TAC). An increase in TAC was achieved by 50% in China rose radish sprouts and by 30% in Rambo red radish after glucose treatment. Methyl jasmonate and sucrose also contribute to enhance TAC. Enriching natural food in anthocyanins may contribute to sustain their regular intake with preventive and therapeutic roles in a number of human diseases.
- PublicationOpen AccessThe preventive effects of broccoli bioactives against cancer: Evidence from a validated rat glioma model(Elsevier, 2023-10-13) Baenas, Nieves; Vega-García, Angélica; Manjarrez-Marmolejo, Joaquín; Moreno, Diego A.; Feria-Romero, Iris A.The aggressive and incurable diffuse gliomas constitute 80% of malignant brain tumors, and patients succumb to recurrent surgeries and drug resistance. Epidemiological research indicates that substantial consumption of fruits and vegetables diminishes the risk of developing this tumor type. Broccoli consumption has shown beneficial effects in both cancer and neurodegenerative diseases. These effects are partially attributed to the isothiocyanate sulforaphane (SFN), which can regulate the Keap1/Nrf2/ARE signaling pathway, stimulate detoxifying enzymes, and activate cellular antioxidant defense processes. This study employs a C6 rat glioma model to assess the chemoprotective potential of aqueous extracts from broccoli seeds, sprouts, and inflorescences, all rich in SFN, and pure SFN as positive control. The findings reveal that administering a dose of 100 mg/kg of broccoli sprout aqueous extract and 0.1 mg/kg of SFN to animals for 30 days before introducing 1 × 104 cells effectively halts tumor growth and progression. This study underscores the significance of exploring foods abundant in bioactive compounds, such as derivatives of broccoli, for potential preventive integration into daily diets. Using broccoli sprouts as a natural defense against cancer development might seem idealistic, yet this investigation establishes that administering this extract proves to be a valuable approach in designing strategies for glioma prevention. Although the findings stem from a rat glioma model, they offer promising insights for subsequent preclinical and clinical research endeavors.