Browsing by Subject "Seborrheic keratosis"

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    Relationship of human papillomavirus with seborrheic keratosis of the female genital tract - a case-series and literature review
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2021) Dasgupta, Shatavisha; Eersel, Rachel van; Morrel, Beth; Munckhof, Henk A.M. van den; Geus, Vera A. de; Hoeven, Nick M.A. van der; Sandt, Miekel M. van de; Piso-Jozwiak, Marta; Quint, Wim G.V.; Avoort, Irene A.M. van der; Koljenović, Senada; Ewing-Graham, Patricia C.; Kemenade, Folkert J. van
    Seborrheic keratoses (SKs) are benign lesions of uncertain etiology, which can develop in both genital and extra-genital locations. For genital SKs there has been conjecture about the pathogenic role of human papillomavirus (HPV), in view of the frequent association of this virus with genital lesions. In light of the potential consequences on patient management, we investigated the relationship between HPV and SKs of the female genital tract (FGT). For this, we evaluated the current evidence on this relationship by performing an in-depth review of the literature. Furthermore, to add to the evidence on this association, we investigated the presence of HPV in a series of vulvar SKs (n=15), using a novel multimodal approach. This involved whole tissue section-polymerase chain reaction (WTS-PCR) using SPF10-DEIA-LiPA25 for HPV detection and genotyping. In addition, immunohistochemistry (IHC) was performed with cellular biomarkers p16 and MIB-1, and viral biomarker E4, to augment HPV-testing. Finally, laser-capture microdissection-PCR (LCM-PCR) was performed to locate HPV to specific lesional cells, and to rule out incidental detection of resident HPV with WTS-PCR. Our findings from the literature review as well as the case-series are presented.
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    The oligosaccharidic component of the glycoconjugates in lichen planus, granuloma annulare, seborrheic keratosis and palmoplantar keratoderma: lectin histochemical study
    (Murcia : F. Hernández, 1999) Gheri, G.; Russo, G.; Cappugi, P.; Sgambati, E.; Gheri Bryk, S.
    It is well known that cell surface glycoconjugates play an important role in cell proliferation, adhesion and differentiation. The aim of this investigation was to define the changes of the glycoconjugate saccharidic moieties in the epidermis and derma of patients affected by several skin pathologies such as seborrheic keratosis, lichen planus, granuloma annulare and palmoplantaris keratoderma. Bioptical specimens from skin lesions as well as from normal skin were fixed in Carnoy's fluid and routinely processed. The sections were treated with HRP-lectins (PNA, DBA, SBA, WGA, ConA, LTA and UEAI). Cytochemical controls were performed for specificity of lectin-sugar reaction. Some sections were pre-treated with neuraminidase prior to staining with I-IRP lectins. In comparison with normal human skin, epidermal lectin binding pattern in the considered diseases showed considerable qualitative and quantitative variations. In general, in all the considered pathologies, a lack andtor a decrease in lectin binding at the epidermal layers was observed; among the various diseases, differences in cellular localisation of the sugar residues were also noted. In such respect, an exception was represented by seborrheic keratosis, where the cells of the basal layer showed PNA reactivity, which was absent in the basal layer of the normal skin. Although seborrheic keratosis and lichen planus have been studied by others authors, our findings are not in total accordance concerning lectin binding; this is probably due to the different fixatives employed. Our findings seem to reveal significant changes in keratinocyte glycoconjugate oligosaccharides in the previously mentioned diseases, providing clues to their pathogenesis.