Browsing by Subject "Renal cortex"
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- PublicationOpen AccessAlleviative effect of myricetin on ochratoxin A-induced oxidative stress in rat renal cortex: histological and biochemical study(Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Abd El-haleem, Manal R.; Kattaia, Asmaa A.A.; Abd El-Baset, Samia A.; El Sayed Mostafa, HebaOchratoxins (OTA) are secondary metabolites of Aspergillus and Penicillium. The detoxification of OTA has been of major interest due to its widespread threat to human health. We aimed to investigate the possible alleviative effect of myricetin (MYR) against OTA-induced damage in renal cortex of rats. Thirty adult male albino rats were randomized into five equal groups: control (untreated), vehicle control (0.5 ml corn oil/day including dimethylsulfoxide [DMSO]), MYR (100 mg MYR/kg b.w./day in distilled water), OTA (0.5 mg OTA/kg b.w./day; dissolved in 10% DMSO and then corn oil) and OTA + MYR group (received OTA and MYR at similar doses). All treatments were given by oral gavage for 2 weeks. At the end of the experiment, renal cortices were processed for light and electron microscope examinations. Immunohistochemical staining for localization of proliferating cell nuclear antigen (PCNA), p53 and transforming growth factor beta 1 (TGF-β1) was carried out. Biochemical analysis of tissue glutathione peroxidase (GPX), catalase (CAT) and superoxide dismutase (SOD) were determined to evaluate oxidative stress. OTA administration induced deleterious renal injury evidenced by the structural and ultra-structural changes. Immunohistochemical expression of p53, PCNA and TGF-β1 were significantly up regulated compared with control. Alterations in antioxidant parameters supported that oxidative stress was one of the mechanisms involved in OTA toxicity. On the contrary, co-administration of MRY partially ameliorated OTAinduced renal injury. We suggest the potential effectiveness of MYR to counteract OTA-induced toxic oxidative stress on the renal cortex.
- PublicationOpen AccessHigh resolution SEM analysis of acellular glomerular basement membrane following pepsin digestion, intrinsic fibrillar structures(Murcia : F. Hernández, 1990) Berger, Walter J.; Carlson, E. C.Microdissection of acellular rat renal cortex with pepsin was carried out to investigate the morphological substructure of glomerular basement membrane (GBM) by high resolution SEM. Renal cortical blocks (< 5 mm ) from adult male Sprague Dawley rats were rendered acellular by sequential detergent extraction and digested up to 184 hrs with 5 mglml pepsin (185 U/ mg) in 0.5 M acetic acid (pH 2) at 10-15°C. Samples were conventionally prepared for SEM, and observed at original magnifications of 500-100,000 diameters. At low magnifications (500-5,00Ox), acellular GBM surfaces appeared smooth at al1 digestion times. At higher magnifications (50,000-100,00Ox), control GBM surfaces were finely granular. Granule diameter ranged from 20-80 nm, with most between 30-40 nm. Pepsin digestion did not affect average granule size. Beginning at 44 hrs of digestion, intrinsic fibrillar structures comprised of linear arrays of 20-40 nm granules were observed onlin GBM surfaces. At later incubation times, this component of GBM became more extensive. At 160 hrs, the fibrillar arrays frequently bifurcated and showed distinctive «forked» termini, some of which comprised two sides of a triangle (120-150 nm on a side). Fork «handles» (310-350 nm in length) radiated from each angle of the triangle. These sometimes terminated in large granules (approximately 100 nm in diameter), two of which appeared to connect fibrillar arrays end-toend. Together with other arrays, the interconnected triangles appeared to comprise a three-dimensional meshwork extending into the GBM and possibly providing support for, its granular components.
- PublicationOpen AccessUltrastructural changes of the renal cortex after septic shock in rats(Murcia : F. Hernández, 1988) Achparaki, A.; Kotzampassi, K.; Eleftheriadis, E.; Foroglou, Ch.