Browsing by Subject "Ratones"

Now showing 1 - 3 of 3
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    A method to collect to high volumes of milk from mice (Mus musculus)
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2013) Gómez Gallego, Carlos; Ilo, T.; Ulla-Marjut, Jaakkola; Salminen, Seppo; Periago Castón, María Jesús; Ros Berruezo, Gaspar Francisco; Frías Beneyto, Rafael; Facultad de Veterinaria
    ABSTRACT Collecting milk samples from mice (Mus musculus) may be interesting for a variety of preclinical research. References in the literature for protocols describing how to milk a dam are scarce, and a major limitation of such protocols is the small sample volume that is generally collected. The aim of our study was to develop a practical protocol to collect substantial amounts of milk from mice. Adult female outbred NMRI and inbred BALB/c mice with nursing litters were used in this study. The milking was carried out on days 7–12 after parturition. The pups were separated from their mothers for 6–12 h before milking to allow accumulation of milk in the glands. Dams were anesthetized using either an injectable mixture of midazolam and ketamine, or by use of the inhalational agent isoflurane. To induce milk flow, the mice were given 2-8 IU of oxytocin intraperitoneally. The milk was collected using an electric human breast pump that was modified to accommodate mouse nipples and to handle small liquid volumes. With this protocol, the total amount of milk collected from each dam per each milking ranged between 0.2 and 1.5 mL. We concluded that this milking method provides an excellent means for acquiring substantial amounts of mouse milk.
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    Estudio inmunohistopatológico del bazo de ratones deplecionados de linfocitos t (cd4+ y cd8+) e infectados por chlamydophila abortus
    (Murcia: Universidad de Murcia, Servicio de Publicaciones, 2006) Martínez Gracia, Carmen; Sánchez Campillo, Joaquín; Buendía Marín, Antonio Julián; Facultad de Veterinaria
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    P2X7 receptor differentially couples to distinct release pathways for IL-1b in mouse macrophage
    (The American Association of Immunologists, Inc., 2008) Pelegrin, Pablo; Barroso-Gutierrez, Consuelo; Surprenant, Annmarie; Bioquímica y Biología Molecular B e Inmunología
    The pro-inflammatory IL-1 cytokines, IL-1a, IL-1b and IL-18, are key mediators of the acute immune response to injury and infection. Mechanisms underlying their cellular release remain unclear. Activation of purinergic P2X7 receptors (P2X7R) by extracellular ATP is a key physiological inducer of rapid IL-1brelease from LPS-primed macrophage. We investigated patterns of ATP-mediated release of IL-1 cytokines from three macrophage types in attempts to provide direct evidence for or against distinct release mechanisms. We used peritoneal macrophage from P2X7R-/- mice and found that release of IL-1a, IL-18, as well as IL-1b, by ATP resulted exclusively from activation of P2X7R, that release of all these IL-1 cytokines involved pannexin-1 (panx1), and that there was both a panx1-dependent and independent component to IL-1b release. We compared IL-1 release patterns from LPS-primed peritoneal macrophage, RAW264.7 macrophage and J774A.1 macrophage. We found RAW264.7 macrophage readily release pro-IL- 1b independently of panx1 but do not release mature IL-1b because they do not express apoptotic speck-like protein with a caspase-activating recruiting domain (ASC) and so have no caspase-1 inflammasome activity. We delineated two distinct release pathways: the well-known caspase-1 cascade mediating release of processed IL-1b that was selectively blocked by inhibition of caspase-1 or panx1, and a calcium-independent, caspase-1/panx1-independent release of pro-IL-1b that was selectively blocked by glycine. None of these release responses were associated with cell damage or cytolytic effects. This provides the first direct demonstration of a distinct signaling mechanism responsible for ATP-induced release of pro-IL-1b.