Browsing by Subject "Proinflammatory cytokines"

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    Broccoli byproduct extracts attenuate the expression of UVB-induced proinflammatory cytokines in HaCaT keratinocytes
    (MDPI, 2024-12-02) Borja Martínez, María; Pedreño García, María Ángeles; Sabater Jara, Ana Belén; Biología Vegetal
    Broccoli byproducts are an important source of bioactive compounds, which provide important benefits for human skin due mainly to their antioxidant and anti-inflammatory properties. The primary target of UVB radiation is the basal layer of cells in the epidermis, with keratinocytes being the most abundant cell population in this layer. Given the wide range of side effects caused by exposure to UVB radiation, reducing the amount of UV light that penetrates the skin and strengthening the protective mechanisms of the skin are interesting strategies for the prevention of skin disorders. This work aims to evaluate the protective mechanisms triggered by broccoli by-products extract (BBE) on HaCaT keratinocytes exposed to UVB radiation as well as the study of the regenerative effect of these extracts on the barrier of skin keratinocytes damaged by superficial wounds as a strategy to revalorize this agricultural waste. The results obtained revealed that the BBEs exhibited a high cytoprotective effect on the HaCaT exposed to UVB light, allowing it to effectively reduce the intracellular content of ROS, as well as effectively attenuating the increase in proinflammatory cytokines (IL-1β, IL-6, IL-78, TNF-α) and COX-2 induced by this type of radiation. Furthermore, the BBE could be an excellent regenerative agent for skin wound repair, accelerating the migration capacity of keratinocytes thus contributing to the valorization of this byproduct as a valuable ingredient in cosmetic formulations.
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    Immunostaining of proinflammatory cytokines in renal cortex and medulla of rats exposed to gold nanoparticles
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2017) Khan, Haseeb A.; Ibrahim, Khalid E.; Khan, Ayaat; Alrokayan, Salman H.; Alhomida, Abdullah S.
    Recently, gold nanoparticles (GNPs) have shown promising applications in targeted drug delivery and contrast imaging. Although in vitro cytotoxicity of GNPs has been thoroughly studied, there are limited data on in vivo toxicity of GNPs. In this study, we evaluated the effects of intraperitoneally injected 10 nm and 50 nm GNPs (5 µg/animal) on the expression of proinflammatory cytokines (IL-1β, IL-6 and TNF-α) on day 1 and day 5, post-exposure. The results of immunohistochemistry showed that both 10 nm and 50 nm GNPs induced an acute phase expression of proinflammatory cytokines in renal cortex and medulla. This proinflammatory response was comparatively more intense in renal medulla than cortex. All the three cytokines were undetectable in control cortex and medulla. In conclusion, both 10 nm and 50 nm GNPs caused an acute phase induction of proinflammatory cytokines in cortex and medulla of rat kidneys. An intense immunostaining of proinflammatory cytokines in renal medulla warrants further studies to evaluate the nephrotoxicity of GNPs to validate the safe application of GNPs for contrast imaging in renal insufficiency.
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    Therapeutic potential of porphyran in mitigating ischemia-reperfusion injury in gerbil hippocampus
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2026) Tae-Kyeong Lee; Joon Ha Park; Dae Won Kim; Choong-Hyun Lee; Moo-Ho Won; Il Jun Kang; Ji Hyeon Ahn; Biología Celular e Histología
    Cerebral ischemia-reperfusion (IR) injury is a critical pathological event that leads to extensive neuronal loss, neuroinflammation, and blood-brain barrier (BBB) dysfunction. Porphyran, a sulfated polysaccharide derived from Porphyra spp., has demonstrated anti-inflammatory and neuroprotective effects in various neurological conditions. This study aimed to evaluate the post-ischemic therapeutic potential of porphyran in a gerbil model of transient forebrain ischemia. Our findings reveal that porphyran administration (50 mg/kg orally once daily for five days) following IR significantly mitigated IR-induced cognitive decline, as evidenced by the Y-maze test, but porphyran treatment did not significantly prevent neuronal death in the CA1 subregion of the hippocampus, as revealed by Cresyl Violet (CV) and Fluoro-Jade B (FJB) staining. However, porphyran treatment after IR injury effectively attenuated the IR-induced decrease in acetylcholine (ACh) levels, suggesting potential preservation of cognitive function in surviving neurons. Furthermore, porphyran significantly mitigated microglial activation and reduced the levels of proinflammatory cytokines (IL-1β, IL-6, and TNF-α), indicating its anti-inflammatory properties. Additionally, porphyran administration reduced BBB disruption, as evidenced by decreased extravasation of immuno-globulin G (IgG), suggesting a role in maintaining vascular integrity. In summary, although porphyrin administration after IR does not protect pyramidal neurons directly, it may improve cognitive function by mitigating ACh depletion, suppressing microglial activation, and reducing inflammatory cytokine levels