Browsing by Subject "Plant extracts"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Publication
    Restricted
    Evaluating the efficacy of cinnamaldehyde and Echinacea purpurea plant extract in broilers against Eimeria acervulina
    (Elsevier, 2011-09-15) Orengo, Juan; Ruiz de Ybáñez Carnero, María del Rocío; Madrid, Josefa; del Río, Laura; Catalá-Gregori, Pablo; García, V.; Hernández, Fuensanta; Buendía Marín, Antonio Julián; Sanidad Animal
    Coccidiostats could be phased out as feed additives before 1 January 2013 for public health and food safety reasons, and, as a replacement, bioactive compounds found in plants are currently being investigated since they are more likely to be found acceptable by consumers. The aim of our study was to evaluate the effect of cinnamaldehyde (CIN) and Echinacea purpurea plant extract (EP) as additives by analyzing the performance traits, oocyst excretion and intestinal lesions following experimental infection with Eimeria acervulina. A total of 72 Ross male broilers were raised from 1 to 35 d and randomly assigned to four dietary treatments: control, without additives (C); 150 mg kg−1 cinnamaldehyde (CIN); 1000 mg kg−1 E. purpurea plant extract (EP); 150 mg kg−1 cinnamaldehyde plus 1000 mg kg−1 E. purpurea plant extract (CIN + EP). At 25 d, 12 chickens per treatment were orally infected with E. acervulina. Coccidia infestation led to lower performance but with no significant differences between the infected groups. Oocyst output reached its peak from 6 to 9 d post-infection in all treatments. At duodenal level, gross lesion scores were lower for cinnamaldehyde diets (P < 0.05). A similar trend was observed in the microscopic lesion scores, with a nonsignificant reduction as a result of cinnamaldehyde addition (P > 0.05). Scoring methods for macro- and microscopic lesions showed a positive linear relationship (G = +0.70). Further studies are necessary to assess the possible anticoccidian action of the cinnamaldehyde and its value as an alternative or adjunct in therapeutic or prophylactic strategies.
  • Repository logo
    Publication
    Open Access
    Use of carrageenan in the screening of natural anti-inflammatory molecules in fish: in vitro effects of Chiliadenus glutinosus extracts
    Campos-Sánchez, Jose Carlos; Esteban Abad, María de los Ángeles; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología
    Carrageenan, a high molecular weight sulphated polysaccharide, has been used in the screening of anti-inflammatory molecules in mammals. In this sense, Chiliadenus glutinosus (L.) Fourr., an endemic species of plant has been reported in the Spanish popular medicine due to their anti-inflammatory properties in humans. However, their properties have poorly been studied in fish. In this assay, different concentrations (0, 0.001, 0.01, 0.125, 0.25, 0.5 and 1 mg mL-1) of aqueous, ethanolic and methanolic extracts of this plant were elaborated, and the antioxidant, bactericidal (against four marine fish pathogen) and cytotoxic activity, as well as several immunological parameters in head-kidney leucocytes (HKLs) of gilthead seabream (Sparus aurata) were evaluated in a first experiment to determinate the extract with the best properties. Our results showed a dose-dependent antioxidant activity of each plant extract increasing with the concentration, a species- and extract-specific bactericidal activity, as well as a cytotoxic activity against the PLHC1 tumour cell line by the ethanolic and methanolic extracts. However, taking into account the cytotoxic effects of these two extracts on HKLs and the strong immunosuppressive effects on the immunological parameters assayed (peroxidase, respiratory burst activity and phagocytic capacity), a second experiment was developed by using only adjusted doses of the aqueous extract (0, 0.25 and 0.5 mg mL-1), in which immune parameters, cell morphology and inflammatory-related genes were studied in HKLs stimulated with λ-carrageenan. The present results offer a detailed view of the biological effects of C. glutinosus extracts, which could be interesting to the aquaculture sector due to their possible anti-inflammatory effects.