Browsing by Subject "Phytochemistry"

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    In vitro culture of the endangered plant Eryngium viviparum as dual strategy for its ex situ conservation and source of bioactive compounds
    (Springer, 2019-06-15) Moreno Ayuso, Juan Manuel; Ramil‑Rego, Pablo; Gallego, Pedro Pablo; Barreal, M. Esther; García Pérez, Pascual; Tecnología de Alimentos, Nutrición y Bromatología; Facultad de Veterinaria
    Different Eryngium species have been used with ornamental, agricultural and medicinal purposes, as a consequence of their chemical constituents. In the southwest Europe the endemic Eryngium viviparum, presents a high risk of extinction and ex situ strategies are high recommended for efficient conservation and re-introduction program. The objective of this study was to satisfy a dual objective: (i) to develop an ex situ conservation strategy through micropropagation and (ii) taking advantage of the extraordinary potential of plant tissue culture, produce a considerable amount of plant material to carry out a preliminary phytochemical study, based on the accumulation of phenolic compounds and their associated antioxidant activity. First a factorial design was conducted in order to study the effect of two cytokinins (6- benzylaminopurine, BAP, and kinetin, KIN), at three levels (0, 1 and 2 mg L−1), on shoot multiplication. Later another factorial design was applied, by using three levels of MS medium salt strength (full, half and quarter- strength) and four sucrose levels (0, 1, 2, and 3%) for improving shoot elongation and rooting. In parallel, a preliminary quantification of total phenolic and flavonoid contents from E. viviparum aerial parts was determined. The simple micropropagation protocol designed allowed obtaining a high rates of shoot multiplication (5.1–5.8 new shoots), rooting (100%) with healthy long roots (3.1–3.5 cm) and plantlet acclimatization (96%). Moderate antioxidant activity was recorded in hydromethanolic extracts from E. viviparum aerial parts. High correlation between total phenolic content and BAP levels in the culture media was found. In conclusion, the micropropagation procedure described here for the endangered E. viviparum can be used as new and very efficient ex situ conservation strategy, and as potential source of antioxidants, conferring an added-value to this plant.
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    Taxonomic value of the leaf micro-morphology and quantitative phytochemistry of Jatropha integerrima Jacq. and Jatropha podagrica Hook. (Euphorbiaceae)-known horticultural plants in Nigeria
    (Universidad de Murcia, 2017) Saheed Kolawole, Opeyemi; Adekilekun Jimoh, Mahboob; Yakubu, Fred; Chukwudi Chukwuma, Emmanuel
    Valor taxonómico de la micromorfología de la hoja y la fitoqímica cuantitativa de Jatropha integerrima Jacq. y Jatropha podagrica Hook. (Euphorbiaceae)-conocidas plantas hortícolas en Nigeria Se examinó la micromorfología y la fitoquímica cuantitativa de J. integerrima y J. podagrica para para identificar caracteres útiles para la delimitación taxonómica, siguiendo protocolos estandarizados, descritos por autores anteriores. Ambas especies son hipostó- micas (paracíticas). Los tricomas (multicelulares, no glandulares) estaban presentes sólo en la superficie adaxial de J. integerrrima y ausentes en otras superficies epidérmicas. Los análisis fitoquímos también señalaron pequeñas diferencias en la cantidad de compuestos bioactivos presentes en ambas especies. El contenido de fenol es el mayoritario en ambas especies: 37.65% en J. integerrima y 36.13% en J. podagrica. Las semejanzas y diferencias de los caracteres epidérmico-foliares y contenidos fitoquímicos pueden ser empleadas para delimitar taxonomicamente ambas especies.