Browsing by Subject "Phytochemicals"

Now showing 1 - 4 of 4
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    Anti-leukemic activity of Brassica-derived bioactive compounds in HL-60 myeloid leukemia cells
    (MDPI, 2022-11-02) Núñez-Sánchez, María Ángeles; Martínez-Sánchez, María Antonia; Verdejo-Sánchez, Marina; García-Ibáñez, Paula; Oliva Bolarín, Alba; Ramos-Molina, Bruno; Moreno, Diego A.; Ruiz Alcaraz, Antonio José; Bioquímica y Biología Molecular B e Inmunología; Facultad de Biología
    Acute myeloid leukemia (AML) is a cancer of the myeloid blood cells mainly treated with chemotherapy for cancer remission, but this non-selective treatment also induces numerous side effects. Investigations with bioactive compounds from plant-derived foods against cancer have increased in the last years because there is an urgent need to search for new anti-leukemic agents possessing higher efficacy and selectivity for AML cells and fewer negative side effects. In this study, we analyzed the anti-leukemic activity of several phytochemicals that are representative of the major classes of compounds present in cruciferous foods (glucosinolates, isothiocyanates, hydroxycinnamic acids, flavonols, and anthocyanins) in the human acute myeloid leukemia cell line HL-60. Our results revealed that among the different Brassica-derived compounds assayed, sulforaphane (SFN) (an aliphatic isothiocyanate) showed the most potent anti-leukemic activity with an IC50 value of 6 µM in dose-response MTT assays after 48 h of treatment. On the other hand, chlorogenic acid (a hydroxycinnamic acid) and cyanidin-3-glucoside (an anthocyanin) also displayed anti-leukemic potential, with IC50 values of 7 µM and 17 µM after 48 h of incubation, respectively. Importantly, these compounds did not show significant cell toxicity in macrophages-like differentiated cells at 10 and 25 µM, indicating that their cytotoxic effects were specific to AML cancer cells. Finally, we found that these three compounds were able to induce the NRF2/KEAP1 signaling pathway in a dose-dependent manner, highlighting SFN as the most potent NRF2 activator. Overall, the present evidence shed light on the potential for using foods and ingredients rich in anticancer bioactive phytochemicals from Brassica spp.
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    Antidiabetic effect of Atriplex halimus L. (Sp. Pl. 2: 1052 (1753)) long and short term treatment against streptozotocin induced diabetes in rats
    (Universidad de Murcia. Servicio de publicaciones, 2022) Bounouar, Elaid; Missoun, Fatiha; Ouda Amari, Nesrine; Zohra Belabaci, Fatima; Belabaci, Senia; Zohra Sekkal, Fatima; Djebli, Noureddine
    El objetivo fue evaluar la actividad antidiabética de hojas de Atriplex halimus en ratas diabéticas modelo. Para evaluar se utilizó la glicemia, pérdida de peso, volumen de consumo de agua, parámetros bioquímicos. estudio histológico. Las investigaciones fitoquímicas indican la presencia de flavonoides, taninos, saponinas, mucílagos, glucósidos. proteínas. Los resultados muestran que el tratamiento con extracto acuoso de A. halimus presenta reducción significativa de los niveles de glucosa en sangre en ratas de los grupos D100. D200, en comparación con el grupo diabético,. protege. las ratas de complicaciones diabéticas. El estudio histológico del páncreas lo confirman por la mejora en los islotes de Langerhans de rata tratada con este extracto vegetal. Atriplex halimus parece ser una planta prometedora para futuros ensayos preclínicos. clínicos en la diabetes de tipo I.
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    Assessment of the foliar micromorphology, phytochemical and mineral composition of Justicia secunda Valh leaves
    (Universidad de Murcia. Servicio de publicaciones, 2020) Oluwatimilehin Ogunbamowo, Paul; Bayonle Olaniyi, Musbau; Lanre Awotedu, Olamilekan; Oduola Lawal, Ibraheem
    Evaluación de la micromorfología foliar, fitoquímica y composición mineral de las hojas de Justicia secunda Valh Justicia secunda es una planta medicinal que pertenece a la familia Acanthaceae, es muy utilizada en la medicina tradicional para el tratamiento y manejo de una amplia gama de dolencias. En este estudio, la selección/análisis de fitoquímicos, minerales y características micro-morfológicas de las hojas de J. secunda se evaluaron utilizando procedimientos estándar. Los datos generados para el contenido fitomineral y fitoquímico de las hojas así como la prueba quimio-microscópica y los rasgos anatómicos foliares de las hojas que revelaron rasgos característicos distintos serán de utilidad en la sistemática, adecuada identificación, autenticación y estandarización de esta planta para fines medicinales propósitos.
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    Design, synthesis and gene modulation insights into pigments derived from tryptophan-betaxanthin, which act against tumor development in Caenorhabditis elegans
    (MDPI, 2023-12-20) Henarejos Escudero, Paula; Méndez-García, Fernando F.; Hernández García, Samanta; Martínez Rodríguez, Pedro; Gandía Herrero, Fernando; Bioquímica y Biología Molecular A
    The use of betalains, which are nitrogenous plant pigments, by the food industry is widespread and reflects their safety after intake. The recent research showed outstanding results for L-tryptophan-betaxanthin, a phytochemical present in traditional Chinese medicine, as an antitumoral agent when the activity was evaluated in the animal model Caenorhabditis elegans. Thus, L-tryptophanbetaxanthin is now presented as a lead compound, from which eleven novel structurally related betaxanthins have been designed, biotechnologically produced, purified, and characterized. The antitumoral effect of the derived compounds was evaluated on the JK1466 tumoral strain of C. elegans. All the tested molecules significantly reduced the tumoral gonad sizes in a range between 31.4% and 43.0%. Among the novel compounds synthesized, tryptophan methyl ester-betaxanthin and tryptophan benzyl ester-betaxanthin, which are the first betalains to contain an ester group in their structures, caused tumor size reductions of 43.0% and 42.6%, respectively, after administration to the model animal. Since these were the two most effective molecules, their mechanism of action was investigated by microarray analysis. Differential gene expression analysis showed that tryptophan methyl ester-betaxanthin and tryptophan benzyl ester-betaxanthin were able to down-regulate the key genes of the mTOR pathway, such as daf-15 and rict-1.