Browsing by Subject "Photoperiod"

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    Estudio de la microbiota del insecto tenebrio molitor en diferentes condiciones de luz y con dos dietas basadas en subproductos de la industria alimentaria
    (Universidad de Murcia. Servicio de publicaciones, 2022) Angel Sánchez, Antonio; Galián Albaladejo, José
    La microbiota intestinal de los insectos se puede definir como la comunidad de microorganismos que viven en el tracto intestinal, y que realiza funciones básicas en los procesos digestivos y del sistema inmune. El objetivo de este estudio es el de analizar la microbiota de larvas de Tenebrio molitor, insecto autorizado para su consumo en la UE, en distintas condiciones de luz y con dos dietas. Se estudia el efecto en la microbiota al someterse a luz continua, fotoperiodo, oscuridad continua y luz roja continua, y dos dietas diferentes a base de subproductos de la industria alimentaria, una compuesta por 80% de brócoli y 20% de avena, y otra de 80% de posos del café y 20% de avena. En todos los tratamientos predominan los filos Tenericutes, Proteobacteria, Firmicutes y Cyanobacteria; y en menor proporción, Bacteroidetes y Actinobacteria. Como conclusión, se determinó que diferentes condiciones de luz no muestran variación en la composición bacteriana de T. molitor, sin embargo, distintas dietas modifican su microbiota intestinal. En la de dieta de brócoli predominan Tenericutes (media de 41,9%) y Firmicutes (31,3%), mientras que en la de posos de café son mayoritarias bacterias del filo Proteobacteria (62%).
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    HSP47 expression in the hamster Sertoli cell: An immunohistochemical study
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2024) Serrano-Sánchez, María Isabel; Ferrer, Concepción; Beltrán Frutos, Ester; Martínez Hernández, Jesús; Pastor García, Luis Miguel; Seco Rovira, Vicente
    HSP47, a chaperone whose main function is the maturation of collagen molecules, is considered a marker of fibrotic diseases. Increased collagen synthesis in the testis has been associated with various pathologies leading to seminiferous tubule regression. Our aim was to study whether HSP47 is expressed in hamster Sertoli cells both in the adult and in two physiological situations of seminiferous tubule atrophy: irreversible testicular ageing and testicular regression due to short photoperiod (reversible). Eighteen animals were divided as follows: a group of 6 young animals aged 6 months, a group of 6 animals aged 24 months, which were exposed to a long photoperiod, and a final group of 6 young animals subjected to a short photoperiod. Testicular samples were fixed in methacarn and an immuno-histochemical technique was used to detect HSP47. A semiquantitative study of this protein expression was performed between tubular sections of aged animals with complete spermatogenesis and arrested spermatogenesis and tubular sections with arrest spermatogenesis of photoinhibited testes. Sertoli cells were positive for HSP47, the intensity being greater in tubular sections with arrested spermatogenesis in both aged and photoinhibited animals. Semiquantitative analysis corroborated this observation in the sense that the expression of this protein differed according to the functional state of the seminiferous tubules. Thus, the ratio of immunoreactivity was significantly higher in tubular sections with arrested spermatogenesis in aged animals compared with regressed animals, and in the latter compared with those whose tubular sections showed complete spermatogenesis. In conclusion, HSP47 expression in Sertoli cells was found for the first time in mammals. Moreover, increased expression seemed to be related to the degree of seminiferous atrophy epithelium and to the reversible or nonreversible physiological state of this epithelium.