Browsing by Subject "Oesophagus"

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    Expression of hexokinases and glucose transporters in treated and untreated oesophageal adenocarcinoma
    (Murcia : F. Hernández, 2009) Fonteyne, Philippe; Casneu, Veerle; Pauwels, Patrick; Van Damme, Nancy; Peeters, Marc; Dierckx, Rudi; Van de Wiele, Christophe
    The aim of this study was to assess the expression pattern of the high glucose affinity glucose transporters GLUT 1, 2, 3, 4, 8 and 9 and of hexokinases I, II and III in newly diagnosed oesophageal adenocarcinoma by means of immunohistochemistry. Twenty patients eligible to undergo primary surgery and 18 patients with incomplete pathological response following induction radio chemotherapy, all suffering from oesophageal adenocarcinoma, were included in the study. The intensity and amount of positive tumour cells in the immuno-reaction (histology score (Hscore)) for GLUT 1, 3, 4, 8 and 9 as well as for hexokinase I, II and III were assessed independently by two experienced observers, blinded to the clinical results. In patients that underwent primary surgery, Hscores of GLUT8 (µ 6.7; sd3.3) and GLUT1 (µ 5.5; sd: 5.3) were significantly higher than Hscores of GLUT9 (µ 2.2; sd 1.5) and GLUT3 (µ 3.2, sd: 2.5). Hscores of hexokinase I (µ : 8.3; sd: 4.3), II (µ 5.5, sd: 4.0) and III (Ì 1.5, sd: 0.7) were all significantly different from each other (p<0.04). In patients that underwent radiochemotherapy prior to surgical tumour resection, µ Hscores were 6.9 (sd: 4.4) for GLUT1, 6.8 (sd: 5.3) for GLUT3, 5.9 (sd: 4.2) for GLUT8, 3.4 for GLUT9 (sd: 2.7) and 2.3 (sd: 3.6) for GLUT 4. Hscores of GLUT1 and GLUT3 were significantly higher than Hscores of GLUT4. Finally, Hscores of patients with radiochemotherapy for GLUT3, hexokinase II and III were significantly higher when compared to patients that underwent primary surgery.
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    Quantification of eosinophil densities in the oesophagus, stomach and small bowel of adults: A review of endoscopic and surgical specimens with normal histology, Free State Province, South Africa
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Budding, Liska; Duncan, Jane; Joubert, Gina; Goedhals, Jacqueline
    Aim. Studies defining eosinophil densities in the gastrointestinal tract (GIT) are limited. To assess whether eosinophils are pathologically infiltrating the GIT, it is important to evaluate eosinophil densities for specific populations. Methods. A retrospective, quantitative, comparative study was conducted to determine the number of eosinophils in the oesophagus, stomach and small bowel of patients in central South Africa and to investigate whether a statistically significant difference occurred between ethnic and gender groups. Results. In total, 309 histological sections from the oesophagus, gastric corpus, gastric antrum and small intestine were sampled from male and female, African and Caucasian patients. Histology reports and review of the slides confirmed the absence of histological abnormality. The number of eosinophils in the epithelium and lamina propria were manually quantified. The eosinophil values across gender, ethnicity and location were 0-2.0/mm2 for the oesophagus, 0-53.0/mm2 for the gastric corpus and 7.1-115.3/mm2 for the small intestine. Regarding the gastric antrum, African and Caucasian females had eosinophil values of 1.0-35.7/mm2 and 0-22.4/mm2, respectively. Males had an eosinophil density of 0-31.6/mm2 in the gastric antrum. The eosinophil values in the oesophagus, gastric corpus and small bowel were not significantly different between genders and ethnic groups. The only site where ethnicity influenced the number of eosinophils was the gastric antrum, a discrepancy that cannot be explained. Conclusion. To the authors' knowledge, this is the first report on the eosinophil densities in the oesophagus, stomach and small bowel of adults in South Africa.