Browsing by Subject "Nematode"

Now showing 1 - 5 of 5
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    Complete mitochondrial genome of Metathelazia capsulata (Pneumospiruridae) and comparison with other Spiruromorpha species
    (Springer, 2023-12-04) Aleix-Mata, Gaël; Ruiz de Ybáñez Carnero, M. Rocio; Martínez Carrasco-Pleite, Carlos; Montiel, Eugenia; Sánchez, Antonio; Arcenillas Hernández, Irene; Sanidad Animal
    Metathelazia capsulata (family Pneumospiruridae) is a lungworm parasitizing the bronchi and bronchioles, described in four species of wild carnivores. Very little molecular data are available on this nematode and none on other species of the Pneumospiruridae family. In this work, we describe for the first time the complete mitogenome (mitochondrial genome) of M. capsulata, being the first described of the family Pneumospiruridae. The mitogenome of M. capsulata has 13,659 bp in length, an A + T content of 79.2%. The mitogenome included 12 protein-coding genes (PCGs) (lacking the atp8 gene), 22 tRNA genes, 2 rRNA genes (all the genes are coded by the heavy strand), and an AT-rich region. The PCGs varied in size (232 bp-1645 bp). Only the tRNA-Trp has the standard cloverleaf secondary structure, while the other 21 do not. The AT-rich region, with a 90.5% A + T content and a length of 389 bp, is located between the cox3 and tRNA-Ala genes. Comparison with the mitogenomes of 29 species of Spiruromorpha infraorder, belonging to different families, demonstrates that M. capsulata mitogenome shared the common characteristics of most of them. The phylogeny constructions yielded phylogenies that were in agreement with the obtained previously by using sequences and gene order data of mitogenomes
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    Epidemiological approach to nematode polyparasitism occurring in a sympatric wild ruminant multi-host scenario
    (Cambridge University Press, 2021-06-07) Carrau Garreta, Tessa; Martínez Carrasco-Pleite, Carlos; Garijo Toledo, María Magdalena; Alonso de Vega, Francisco Domingo; León Vizcaino, Luis; Herrera-Russert, José; Tizzani, Paolo; Ruiz de Ybáñez Carnero, María del Rocío; Sanidad Animal
    The epidemiology behind multi-host/multi-parasite systems is particularly interesting to investigate for a better understanding of the complex dynamics naturally occurring in wildlife populations. We aimed to approach the naturally occurring polyparasitism of gastrointestinal nematodes in a sympatric wild ruminant scenario present in south-east Spain. To this end, the gastrointestinal tract of 252 wild ruminants of four different species (red deer, Cervus elaphus; mouflon, Ovis aries musimon; Iberian ibex, Capra pyrenaica and fallow deer, Dama dama) were studied in Cazorla, Segura y Las Villas Natural Park (Andalusia, Spain). Of the analysed animals, 81.52% were positive for parasite infection and a total of 29 nematode species were identified. Out of these, 25 species were detected in at least two host species and 11 parasitized all ruminant species surveyed. The multi-host interaction between these nematodes and the four host species is discussed under the perspective of host family-based differences.
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    First report of Metathelazia capsulata in red foxes (Vulpes vulpes) in Europe and new contributions to its identification
    (Elsevier Ltd., 2023-01) Aleix-Mata, Gael; Sánchez-Baca, Antonio; Martínez Carrasco-Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Arcenillas Hernández, Irene; Sanidad Animal
    Metathelazia capsulata is a lungworm that inhabit in the bronchi and bronchioles from mammal carnivore species, which life cycle is unknown. M. capsulata-like spirurid nematodes were isolated at necropsy from the respiratory tract of red foxes (Vulpes vulpes) from the Region of Murcia (SE Spain). The main objective of this study was to describe in detail the morphometric features of these nematodes, as well as to report some molecular markers. The principal morphometric difference compared to previous M. capsulata descriptions was the shorter total length for both males and females (6.6 mm and 7.4 mm, respectively). In addition, the mean values of buccal cavity depth and distance between the excretory pore and the anterior end of the nematode were also lower than those previously reported. On the other hand, sequence data of the mitochondrial (COI) and nuclear (rDNA) genes of M. capsulata were described, being the first time that molecular markers are reported for the genus Metathelazia and also for the entire family Pneumospiruridae. Based on data available from GenBank, these results indicate that M. capsulata sequences are closely related to the family Rhabdochonidae, which is assumed to belong to superfamily Thelazioidea, a superfamily including the family Pneumospiruridae, but also suggest the distant relations with the family Thelaziidae. This is the first time that M. capsulata is reported in red fox from Europe. This study provides valuable information for future phylogenetic studies on Metathelazia spp. nematodes and, in general, on species of the family Pneumospiruridae.
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    Prevalence of Anguillicoloides crassus (Nematoda, Dracunculoidea) in wild European eels (Anguilla anguilla L.) from Mar Menor lagoon (Western Mediterranean, Spain)
    (École Nationale Vétérinaire de Toulouse, 2011) Martínez Carrasco-Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Peñalver, José; Mayo-Hernández, Elvira; Muñoz, Pilar; García Ayala, Alfonsa; Sanidad Animal
    Anguillicoloides crassus infection in wild European eels (Anguilla anguilla) from Mar Menor, a hypersaline coastal lagoon situated on the shores of theM editerranean in south-east Spain, was evaluated. As well, an artificial digestion is described for the detection, isolation and counting of larval stages in the swimbladder wall. A total of 109 eels were collected between November 2008 and March 2009 and adult worms were recovered from the swimbladders of infected eels. The detected prevalence was 7.34%. Second-stage larvae (L2) numbers ranged from one to thousands per swimbladder. This developmental stage was the most prevalent parasite tsage detected in infected eels. L2 were even found in eels harbouring neither pre-adult nor adult nematodes, which could indicate that infected eels are chronically infected. The lack of a suitable intermediate host for this nematode or the recent introduction of A. crassus into this environment could be the causes of the low prevalence of this parasite in eels from Mar Menor lagoon.
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    Primer caso de Thelaziosis ocular (Thelazia rhodesi) en toro de lidia en la Península Ibérica
    (Universidad de Murcia. Servicio de publicaciones, 2021) Sanes Vargas, José Manuel; Mas Soler, Alberto; Bueno García, Juan Manuel; Martínez Carrasco-Pleite, Carlos; Seva Alcaraz, Juan
    La thelaziosis bovina está causada por Thelazia rhodesi, T. skrjabini y T. gulosa, aunque la especie más prevalente en Europa es la primera de ellas. Thelazia rhodesi es un nematodo (orden Spirurida, familia The-laziidae) que parasita el globo ocular del ganado vacuno, búfalos, cebúes, bisontes y, con menor frecuencia, caballos y pequeños rumiantes. Se localiza en la superficie corneal, sobre la membrana nictitante y en el saco conjuntival. Tiene un ciclo biológico indirecto, actuando como hospedadores intermediarios dípteros de la familia Muscidae. En este estudio se describe un caso de thelaziosis ocular en un toro de lidia (Bos taurus), muerto en la plaza de toros de Murcia en septiembre de 2014, procedente de una ganadería localizada en el su-roeste de la Península Ibérica. Durante el primer y segundo reconocimiento veterinario del animal en la plaza de toros, se observó la existencia de lagrimeo abundante, pero a pesar de ello el animal reaccionaba positivamente a estímulos visuales realizados en el campo visual de ambos ojos, conjunta e independientemente. A pesar del lagrimeo constante, el animal fue considerado apto para la lidia. Durante esta no se observó anormalidad alguna en el comportamiento del toro en relación con el sentido de la vista. La inspección ocular post mortem realizada en el desolladero de la plaza permitió detectar la presencia de nematodos de 8-20 mm de longitud tanto en el saco conjuntival como en la superficie de la córnea, que fueron extraídos con ayuda de pinzas y conservados en etanol al 70%. La identificación microscópica específica de los ejemplares encontrados se realizó en base a los criterios morfométricos descritos por Gupta y Kalia (1978) y Naem (2007a,b), siendo identificados como Thelazia rhodesi. Se trata de un nematodo con tropismo ocular que puede aparecer en el toro de lidia, gana-derías explotadas en régimen extensivo en zonas templadas o cálidas, cuyos condicionantes ambientales van a favorecer la proliferación de los hospedadores intermediarios y el desarrollo de la parasitosis. Por ello, la thelaziosis ha de considerarse en el diagnóstico diferencial de patologías oculares del toro de lidia cuyo cuadro sintomático incluya intranquilidad, agudeza visual disminuida, epífora y prurito en la zona periocular. En el conocimiento de los autores, esta es la primera vez que se publica un caso de parasitación por Thelazia rhodesien el bovino de lidia.