Browsing by Subject "Nasal type"

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    Expression of TIM-3 and LAG-3 in extranodal NK/T cell lymphoma, nasal type
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2018) Feng, Yuhua; Zhong, Meizuo; Liu, Yiping; Wang, Leyuan; Tang, Youhong
    Objective. To investigate the expression of TIM-3 and LAG-3 in extranodal NK/T cell lymphoma, nasal type (ENKTL), and evaluate the clinical and prognostic significance of TIM-3 and LAG-3 in ENKTL. Methods. A total of 61 human paraffin-embedded specimens including 41 ENKTL and 20 rhinitis were involved. We performed immunohistochemistry to detect the expression of TIM-3 and LAG-3. We analyzed correlation between expression of TIM-3 and LAG-3 and clinicopathological features of ENKTL. Results. TIM-3 was positively expressed in 95 (39/41) ENKTL compared with 55% (11/20) in rhinitis, LAG-3 was positively expressed in 95 (39/41) ENKTL compared with 45% (9/20) in rhinitis. A positive correlation was found between TIM-3 and LAG-3. TIM3 and LAG-3 had no significant correlation with ENKTL patients’ age, gender, international prognostic index (IPI) score, B symptoms, LDH level, Ann Arbor stage and treatment regimens. High expression of TIM-3, high IPI score, elevated LDH level and late Ann Arbor stage were shown to be correlated with worse progression free survival (PFS). A multivariate COX regression model show that TIM-3 high expression rate was an independent prognostic factor for ENKTL patients’ PFS. Conclusions. Our findings indicate that TIM-3 might be a promising predictive indicator for the ENKTL patients.
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    Open Access
    Interferon regulatory factor 8 expression and features in blastic plasmacytoid dendritic cell neoplasm and extranodal NK/T-cell lymphoma, nasal type
    (2025) Xiaoqin Dai; Li Sun; Ge Tang; Yu Dong; Fenfen Zhang; Anjia Han; Yuejiao Lang; Biología Celular e Histología
    Aims. To investigate the diagnostic value of Interferon regulatory factor 8 (IRF8) in blastic plasmacytoid dendritic cell neoplasm (BPDCN) and extranodal NK/T-cell lymphoma, nasal type (ENKTL). Methods. Immunohistochemistry staining was used to detect IRF8 expression in 19 cases of BPDCN and 59 cases of ENKTL. In addition, 21 cases of myeloid sarcoma, 30 of B-lymphoblastic leukemia/lymphoma (B-ALL/LBL), 30 of T-lymphoblastic leukemia/lymphoma (T-ALL/LBL), 10 of histiocytic sarcoma, 10 of Langerhans cell histiocytosis, and 9 of follicular dendritic cell sarcoma were also included. DNA sequencing detected IRF8 genetic variation in 6 cases of BPDCN and 20 cases of ENKTL. Results. IRF8 expression was detected in 100.00% (19/19) of BPDCN, exhibiting a strong and uniform staining pattern, and in 91.53% (54/59) of ENKTL, with varying degrees of staining intensity. Weak and focal staining was detected in 33.33% (7/21) of myeloid sarcoma, 13.33% (4/30) of B-ALL/LBL, and 11.11% (1/9) of follicular dendritic cell sarcoma. No expression was found in T-ALL/LBL, histiocytic sarcoma, or Langerhans cell histiocytosis. The proportion of IRF8 positive expression was higher in BPDCN and ENKTL than in other hematolymphoid neoplasms. In ENKTL, the average IRF8 expression was higher in nasal cases than in extranasal cases and in cases with mitosis figures of more than 4/10 high-power field (HPF). Predominant-ly large transformed cell morphology and extranasal involvement site might serve as independent prognostic factors of two-year survival in ENKTL. IRF8 genetic point mutations were found in 33.33% (2/6) of BPDCN and 10.00% (2/20) of ENKTL. Conclusion. The study demonstrated the promising value of IRF8 in the diagnosis of BPDCN and ENKTL.