Browsing by Subject "Mycorrhiza"
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- PublicationOpen AccessBeneficial native bacteria improve survival and mycorrhization of desert truffle mycorrhizal plants in nursery conditions(Springer, 2016-06-04) Navarro-Ródenas, Alfonso; Berná Amorós, Luis Miguel; Lozano-Carrillo, Cecilia; Andrino, Alberto; Morte Gómez, María Asunción; Biología VegetalSixty-four native bacterial colonies were isolated from mycorrhizal roots of Helianthemum almeriense colonized by Terfezia claveryi, mycorrhizosphere soil, and peridium of T. claveryi to evaluate their effect on mycorrhizal plant production. Based on the phylogenetic analysis of the 16S rDNA partial sequence, 45 different strains from 17 genera were gathered. The largest genera were Pseudomonas (40.8 % of the isolated strains), Bacillus (12.2 % of isolated strains), and Varivorax (8.2 % of isolated strains). All the bacteria were characterized phenotypically and by their plant growth-promoting rhizobacteria (PGPR) traits (auxin and siderophore production, phosphate solubilization, and ACC deaminase activity). Only bacterial combinations with several PGPR traits or Pseudomonas sp. strain 5, which presents three different PGPR traits, had a positive effect on plant survival and growth. Particularly relevant were the bacterial treatments involving auxin release, which significantly increased the root-shoot ratio and mycorrhizal colonization. Moreover, Pseudomonas mandelii strain 29 was able to considerably increase mycorrhizal colonization but not plant growth, and could be considered as mycorrhiza-helper bacteria. Therefore, the mycorrhizal roots, mycorrhizosphere soil, and peridium of desert truffles are environments enriched in bacteria which may be used to increase the survival and mycorrhization in the desert truffle plant production system at a semi-industrial scale.
- PublicationOpen AccessCistáceas del Sureste Ibérico con interés por su potencial micorrícico con diversos hongos hipogeos(Universidad de Murcia, Facultad de Biología, 2015) Jiménez, Juan Francisco; Cánovas Rubio, José Luis; Gutiérrez, Almudena; Sánchez Gómez, Pedro; Torrente Paños, María PilarSe aborda el estudio de la familia Cistáceas del Sureste Ibérico, profundizando en aspectos relacionados de su simbiosis micorríci - ca con hongos hipogeos pertenecientes a los géneros Terfezia, que representa junto a Tirmania la mayoría de las turmas consumi - das en el ámbito mediterráneo y de manera secundaria, Picoa y Melanogaster . Se realiza una síntesis de los datos recopilados en la bibliografía especializada, aportando, además, información de campo, alguna de ella inédita, que viene a incrementar y precisar de forma significativa la información disponible hasta la fecha so - bre la relación micorrícica. Destacan los resultados sobre el género Helianthemum , que presenta un total de 21 especies, 12 de las cuales tienen constatada la producción de ascocarpos de Terfezia en condiciones naturales.
- PublicationOpen AccessRoot morphology and mycotrophy of Disperis neilgherrensis (Orchidaceae) from Western Ghats, southern India(Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2013) Muthukumar, Thangavelu; Uma, Eswaranpillai; Raman Pandey, RadhaWe examined the root morphology and mycotrophy of Disperis neilgherrensis Wight., growing in the Western Ghats of south India for the first time. The root system was sparse consisting of white to off white, 0.28 ± 0.11 mm thick roots arising from short brown rhizome. The roots were covered by root hairs (19.2 ± 1.5 per mm of root) 161.80 ± 12.68 μm long and 4.55 ± 1.17 μm thick. Root cortical cells contained fungal structures typical for orchid mycorrhizal (OM) and arbuscular mycorrhizal (AM) types. In contrast, rhizomes contained only OM fungal structures. The OM colonization was characterized by both lightly and darkly staining pelotons with regularly septate hyphae of varied diameters. Intracellular aseptate hyphal coils, arbusculate coils and intracellular vesicles characterized AM colonization. The percentage of length with OM colonization was 56.51% for roots and 73.64% for rhizomes, whereas the root length with AM fungal colonization was 30.23%. The AM type in D. neilgherrensis corresponds to the Paris-type.