Browsing by Subject "Murine model"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    Evaluación de la calidad del cierre de pared abdominal en modelos murinos realizada por alumnos de grado en Medicina.
    (Universidad de Murcia. Servicio de publicaciones, 2022) Gonzalez Lee Chong, Fatima M; Rodriguez Paz, Carlos Agustín; Moreno Ruiz, Alexia; Sánchez de Alba, Luis G; Ramírez Ramos, Kevin
    Resumen: Introducción: Se debe mejorar la docencia quirúrgica, siempre en beneficio del paciente,para que no curse con efectos adversos. Objetivo: Evaluar la calidad del cierre de pared abdominal en modelos murinos realizada por alumnos del grado en medicina. Métodos: Estudio observacional analítico, prospectivo y transversal. Se consideró conjunto universo a 55 alumnos delos cuales solo se incluyen a 35 seleccionados, a los que se les evalúo sobre modelos murinos tiporatas Wistar, tomando como variables el cierre de pared abdominal y las calificaciones según elcuestionario Anaya y Serrano. Resultados: Se evaluaron 35 cierres de pared abdominal, 23 de ellos con calidad (65.7 %) y 12, que no fueron de calidad (34.3 %). Se observa una proporción de cierres de calidad en alumnos aprobados (f=18, 78.3%) frente a no aprobados (f= 5, 21.7%) y también en los cierres de no calidad en alumnos aprobados (f=8, 66.7%) frente a no aprobados (f=4, 33.3%).Conclusión: Los cierres de pared abdominal no exitosos en modelos murinos no se relacionan con las calificaciones reprobatorias.
  • Thumbnail Image
    Publication
    Open Access
    Leishmania amazonensis isolated from human visceral leishmaniasis: histopathological analysis and parasitological burden in different inbred mice
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2018) Freitas de Souza, Celeste da Silva; Calabrese, Kátia da Silva; Abreu Silva, Ana Lúcia; Pereira Carvalho, Luiz Otávio; de Oliveira Cardoso, Flávia; Moraes Cavalheiros Dorval, Maria Elizabeth; Teruya Oshiro, Elisa; Quaresma, Patrícia Flávia; Ferreira Gontijo, Célia Maria; da Silva Pacheco, Raquel; Doria Rossi, Maria Isabel; Gonçalves da Costa, Sylvio Celso; Zaverucha do Valle, Tânia
    Leishmania amazonensis is a major etiological agent of human cutaneous leishmaniasis in the Americas; nevertheless there are some reports of this species causing visceral disease in dogs and men. In the present work we have studied a Leishmania strain isolated from a human case of visceral leishmaniasis. We have infected different mouse strains and analyzed the development of the disease, studying the parasite’s ability to visceralize and whether this ability is influenced by host genetics. Female BALB/c, C57BL/6, C57BL/10, CBA, DBA/2, and C3H/He mice were subcutaneously infected with 104 L. amazonensis amastigotes. BALB/c, C57BL/6 and C57BL/10 mice were found to be very susceptible to infection, showing lesions that developed to necrosis and ulceration. CBA mice developed a late but severe lesion. DBA/2 mice developed only discrete lesions, while C3H/He mice did not develop any lesions. All mouse strains except C3H/He showed some degree of visceralization, presenting parasites in the spleen, while BALB/c, C57BL/6 and CBA presented parasites also in the liver. Moreover, most of the strains presented high parasite load at the infection site, whereas DBA and C3H/He mice showed low or no parasite load 90 days after infection, respectively. Histopathology corroborates the results, showing that susceptible mice presented an inflammatory reaction with parasites in the skin, lymph nodes and spleen, while strains that are more resistant presented low parasitism and discrete inflammatory reaction. Results indicate that this isolate is extremely virulent, can easily visceralize and that the pathogenesis of leishmaniasis is, at least in part, related to the genetic background of the host.
  • Thumbnail Image
    Publication
    Restricted
    Use of photodynamic therapy and chitosan for inactivacion of Candida albicans in a murine model
    (Wiley, 2016-09-03) Camacho Alonso, Fabio; Martínez Beneyto, Yolanda; Gallego, Mª Carmen; Cuello, Francisco; Pérez Lajarín, Leonor; Salinas, Jesús; Buendía Marín, Antonio Julián; Dermatología, Estomatología, Radiología y Medicina Física
    Background The wide use of topical and systemic antifungal agents as the conventional treatment for oral candidiasis has caused Candida albicans to develop resistance to these agents. The aims of this study were to evaluate the effects of photodynamic therapy (PDT) and chitosan on buccal candidiasis and study the possible enhancive effect of chitosan on the photosensitizer methylene blue. Methods Thirty-five DBA/2 immunosuppressed mice were orally inoculated with a suspension of Candida albicans. The animals were randomized into seven groups (n = 5 per group): Group 1 (control); Group 2 (nystatin); Group 3 (PDT); Group 4 (chitosan 1.5 mg/ml); Group 5 (chitosan 3 mg/ml); Group 6 (PDT + chitosan 1.5 mg/ml); and Group 7 (PDT + chitosan 3 mg/ml). The Candida albicans count was evaluated on days 3, 5, 7, and 11 after inoculation. At last, macroscopic and microscopic analyses of the tongue dorsa were performed. Results On day 7 after inoculation, the control group showed a greater number of Candida albicans (5.25 ± 0.41 log10 CFU/ml), with significant differences compared to all other groups (P ≤ 0.05). On day 11 after inoculation, animals treated with PDT showed lower CFU/ml count. Groups 2, 3, 4, 5, and 6 showed fewer microscopic candidiasis lesions than Groups 1 and 7. Conclusions PDT has an antifungal effect, even greater than nystatin. Chitosan has a powerful fungicide effect but did not possess any enhancive effect on methylene blue.