Browsing by Subject "Mastitis"

Now showing 1 - 3 of 3
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    Antibacterial potential of commercial and wild lactic acid bacteria strains isolated from ovine and caprine raw milk against Mycoplasma agalactiae
    (Frontiers Media, 2023-06-22) Toquet, Marion; Bataller, Esther; Gomis Almendro, Jesús; Sánchez López, Antonio; Toledo-Perona, Raquel; de la Fe Rodríguez, Christian; Corrales Romero, Juan Carlos; Gómez-Martin, Ángel; Sanidad Animal
    Introduction: The complexity of fighting contagious agalactia (CA) has raised the necessity of alternative antimicrobial therapies, such as probiotics. Lactic acid bacteria (LAB) are present in the mammary gland of small ruminants and their antimicrobial effect have been previously described against species like Mycoplasma bovis but never against Mycoplasma agalactiae (Ma). This in vitro study aims to evaluate the antimicrobial activity against Ma of ovine and caprine LAB strains and a human commercial probiotic (L2) of Lactobacillus spp. Methods: A total of 63 possible LAB strains were isolated from nine ovine and caprine farms in Spain, three isolates (33B, 248D, and 120B) from the 63 strains were selected, based on their capacity to grow in a specific medium in vitro, for an in vitro experiment to assess their antimicrobial activity against Ma in Ultra High Temperature (UHT) processed goat milk (GM). A women commercial vaginal probiotic was also included in the study. The inoculum of L2 was prepared at a concentration of 3.24 × 108  CFU/mL and the average concentration of the inoculum of the wild LAB varied from 7.9 × 107 to 8.4 × 108  CFU/mL. Results: The commercial probiotic L2 significantly reduced the concentration of Ma to 0.000 log CFU/mL (p < 0.001), strain 33B reduced it from 7.185 to 1.279 log CFU/mL (p < 0.001), and 120B from 6.825 to 6.466 log CFU/mL (p < 0.05). Strain 248D presented a bacteriostatic effect in GM. Moreover, the three wild strains and the commercial probiotic produced a significative reduction of the pH (p < 0.001). Discussion: This is the first in vivo report of the antimicrobial potential of LAB strains against Ma and its interaction. Our results support possible future alternative strategies to antibiotic therapy, previously not contemplated, to fight CA in small ruminants. Further studies are necessary to elucidate the action mechanisms through which these LAB are able to inhibit Ma and to assess the safety of using these strains in possible in vivo studies.
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    Lactancia materna: prevención de problemas tempranos en las mamas mediante una técnica de amamantamiento eficaz
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2013) Fernández Medina, Isabel María; González Fernández, Carmen Tamara
    Introducción: Actualmente la mayoría de las madres conocen la importancia de la lactancia materna tanto para la madre como para el hijo desde el punto de vista nutritivo y de desarrollo así como afectivo y de prevención de patologías; pero algunas madres desconocen que una técnica de lactancia inadecuada puede llegar a ocasionar problemas tempranos en las mamas y con ello, en numerosas ocasiones, el abandono temprano del amamantamiento. Objetivos: En este estudio se plantean las principales alteraciones mamarias puerperales que pueden estar asociadas a una técnica de lactancia ineficaz. Metodología: Se ha realizado una búsqueda bibliográfica en las siguientes bases de datos: Medline, Pubmed y The Cochrane Database of Systematic Reviews. Se incluyen los trabajos relacionados con el objetivo del presente texto. Resultados: Los estudios consultados evalúan la asociación entre la aparición de problemas mamarios y la utilización de una técnica de lactancia inadecuada y la eficacia de los tratamientos tras la aparición de las lesiones. Conclusiones: La asociación entre la aparición de problemas tempranos en las mamas asociados a una técnica de lactancia materna inadecuada es evidente. Se concluye que la prevención es el mejor tratamiento, lugar donde desarrolla una importante labor el profesional sanitario.
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    Susceptibility of caprine mastitis pathogens to tildipirosin, gamithromycin, oxytetracycline, and danofloxacin: effect of serum on the in vitro potency of current macrolides
    (Springer, 2022-09-13) Galecio Naranjo, Juan Sebastian; Escudero Pastor, Elisa; Corrales, Juan Carlos; García-Romero, Edgar; Fe, Christian de la; Hernandis Belenguer, Verónica; Marín Carrillo, Pedro; Farmacología
    Mastitis is a significant disease in dairy ruminants, causing economic losses to the livestock industry and severe risks to public health. Antibiotic therapy is one of the most crucial practices to treat mastitis, although the susceptibility of caprine mastitis pathogens to current antibiotics has not been tested under standard or modified incubation conditions. This work evaluated the in vitro activity of tildipirosin, gamithromycin, oxytetracycline, and danofloxacin against caprine mastitis pathogens incubated following standard conditions of Clinical and Laboratory Standards Institute (CLSI) and deviation method by 25% supplementation with goat serum. Mycoplasma agalactiae, Escherichia coli, Staphylococcus aureus, Streptococcus spp., and coagulase-negative Staphylococci (CNS) were isolated from dairy goats with mastitis in Spain. Minimum inhibitory concentrations (MICs) were determined using the broth microdilution technique. The lowest MIC90 under standard conditions was obtained with danofloxacin for mastitis-causing pathogens. An exception was M. agalactiae, where danofloxacin and oxytetracycline obtained low values. However, after adding serum, gamithromycin showed the lowest MIC50 for S. aureus, Streptococcus spp., and CNS. The lowest MIC50 was obtained with all the antibiotics tested (< 0.125 µg/ml) against M. agalactiae. Supplementing with serum resulted in a significant variation in tildipirosin and gamithromycin MIC values for CNS, S. aureus, M. agalagtiae, and E. coli. In brief, the MIC for antibiotics used against mastitis should be determined under conditions closely resembling intramammary infections to obtain representative susceptibility patterns against mastitis pathogens. Caprine mastitis pathogens were broadly susceptible to danofloxacin under standard conditions. The potency of macrolides against caprine mastitis pathogens increases when serum is present in culture media.