Browsing by Subject "Lungworms"

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    Multivariate Abundance Analysis of Multi-Host/Multi-Parasite Lungworms in a Sympatric Wild Ruminant Population
    (MDPI, 2021-05-23) Carrau Garreta, Tessa; Martínez Carrasco-Pleite, Carlos; Garijo Toledo, María Magdalena; Alonso de Vega, Francisco Domingo; Ruiz de Ybáñez Carnero, María del Rocío; Tizzani, Paolo; Sanidad Animal
    In the analysis of a multi-host/multi-parasite system and its associated risk factors, it is particularly interesting to understand the natural dynamics among pathogens, their hosts, and the environment in wildlife populations. This analysis is particularly feasible in a scenario where multiple overlapping host populations are present in high densities, along with a complex community of parasites. We aimed to describe and analyze the naturally occurring lungworm polyparasitism in a wild ruminant community in Southeast Spain. The respiratory tracts of 250 specimens belonging to four different species (red deer, mouflon, Iberian ibex, and fallow deer) were studied. Almost half (48.0%) of the animals were infected with bronchopulmonary nematodes. Seven different nematodes were identified of which two genera (Protostrongylus spp. and Dictyocaulus spp.) and three additional species (Cystocaulus ocreatus, Muellerius capillaris, and Neostrongylus linearis) were recorded in at least two ruminants, with the mouflon as the commonest host. Our study shows a significant effect of host species and sampling area, plus a marginal effect of age, on parasite multivariate abundance at the host population level. Mouflon and adults of all hosts appear to carry the highest parasite load on average. From a spatial perspective, the highest parasite abundance was detected at the central part of the park.
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    What is the sensitivity and exactness of post-mortem diagnostic method for cardiopulmonary nematodes in wild carnivores? towards the gold standard
    (Springer Nature, 2023-03-15) Ruiz de Ybáñez Carnero, María del Rocío; Martínez-Carrasco Pleite, Carlos; Arcenillas Hernández, Irene; Sanidad Animal
    Cardiopulmonary nematodes cause health and fitness disorders in wild and domestic carnivores. The red fox (Vulpes vulpes) participates in the spread of these shared parasites at the domestic-wildlife interface. This study aimed to evaluate the sensitivity of post-mortem diagnostic method for detecting lungworms in carnivores, and its exactness to estimate the parasite intensity of each nematode species. Cardiorespiratory system of fifty-one foxes were examined through three consecutively methodological steps: first, the tracheobronchial tree, pulmonary arteries and their branches were opened (OT); next, lung parenchyma was immersed in water and squeezed (WS); finally, the parenchyma was artificially digested in a pepsin and chlorhydric acid solution (AD). Eucoleus aerophilus, Angiostrongylus vasorum, Crenosoma vulpis and Metathelazia capsulata were identified. The number of recovered nematodes in each step were 454 (OT), 285 (WS) and 141 (AD). The use of OT and WS helped to improve parasite intensity results and decreased false negative cases. Accordingly, when OT and WS were used together, the sensitivity in the detection of parasitized foxes was 96.1%, while the exactness of parasite intensity was 84%. When AD was performed, although sensitivity does not rise, results were more exact, increasing the total number of detected parasites by 16%. Moreover, AD improved the sensitivity in the detection of A. vasorum and M. capsulata, as well as quantifying more exactly the parasite intensity (92.5% and 92.3% of exactness without AD, respectively). Our study provides valuable information that should be taken into account when planning epidemiological studies based on cardiopulmonary nematode detection in carnivores.