Browsing by Subject "Localization"

Now showing 1 - 3 of 3
  • Thumbnail Image
    Publication
    Open Access
    Cultivando el racismo : procesos de segmentación laboral y de exclusión social en el medio rural andaluz
    (2002) Martín Díaz, Emma; Editora Regional de Murcia
    En este artículo nos proponemos analizar cómo inciden en Andalucía (región objetivo 1 de la Unión Europea) las dinámicas de globalización en relación a la articulación de los procesos migratorios y los mercados de trabajo agrícolas. El estudio de caso nos servirá para ilustrar estas dinámicas en una doble dirección: por una parte, los factores de atracción y de expulsión que conectan sociedades de origen y de destino en una dinámica cada vez más transnacional; por otra, el predominio del mercado determina una pérdida de atribuciones de los Estados que supone, entre otras cuestiones, la desregulación de los mercados de trabajo. La paradoja de un mundo cada vez más "libre" en el intercambio de bienes y servicios, pero con restricciones también cada vez mayores en la libre circulación de los trabajadores, se traduce no en una restricción de esta circulación, sino en una precarización, que supone un endurecimiento de las condiciones de vida y de trabajo de los recién llegados, privados de los derechos ciudadanos y, en un número creciente, invisibilizados e ilegalizados.
  • Thumbnail Image
    Publication
    Open Access
    Histoblot: A sensitive method to quantify the expression of proteins in normal and pathological conditions
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2023) Aguado, Carolina; Martín-Belmonte, Alejandro; Alfaro-Ruiz, Rocío; Martínez Moreno, Ana Esther; Luján, Rafael
    The histoblot (in situ immunoblotting) technique is a simple, reproducible, and sensitive method for protein detection that allows both protein quantitation and analysis of tissue distribution. This easy and fast method allows the direct transfer of native proteins from unfixed frozen tissue sections by mechanical pressure to an immobilizing matrix. Proteins are directly blotted onto nitrocellulose membranes that are then immunolabelled similar to a western blot, but the result is an immunohistochemical imprint of the section retaining all proteins. The histoblot combines advantages of western blot and immunohistochemical methods and yields optimal accessibility of proteins blotted on membranes whilst also preserving anatomical resolution. In addition, it avoids chemical modifications, crosslinking, or semi-denaturation of proteins, which can alter the access of antibody to epitopes, as introduced by conventional immunohistochemistry. Therefore, the histoblot often enables the use of antibodies that do not recognise the target protein in fixed tissue samples. This method has become a trusted alternative to reveal and compare the regional distribution and expression profile of different proteins in the brain in physiological and pathological conditions. In addition, the technique exhibits a high subregional resolution, although is not suitable to unravel protein distribution at the cellular and subcellular levels. In this review, we introduce the histoblot procedure used in our laboratory on brain sections for the identification of quantitative changes of neurotransmitter receptors, ion channels and other signalling molecules in the brain. We also discuss the potentialities, limitations, and fundamental principles of this technique.
  • Thumbnail Image
    Publication
    Open Access
    Localization of Fusobacterium nucleatum in oral squamous cell carcinoma and its possible directly interacting protein molecules: A case series
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2023) He, Xuan; Ma, Xuemeng; Meng, Zijun; Han, Zhiqi; Chen, Wenxia
    Introduction. While 15 to 20% of cancers are associated with microbial infection, the relationship between oral microorganisms and oral squamous cell carcinoma (OSCC) remains unclear. The location of bacteria in a tumor is closely related to its carcinogenic mechanism. The aim of this study was to analyse bacterial diversity in clinical OSCC tissue samples and tumor distant normal tissues, locate target bacteria, and search for proteins that may interact with target bacteria. Materials and Methods. The 16S rDNA method was used to analyse bacterial diversity in clinical OSCC tissue samples and tumor distant normal tissues. Correlations between Fusobacterium abundance and clinicopathological characteristics were analysed using the χ2 test. The position of target bacteria was analysed by fluorescence in situ hybridization (FISH), and the expression of CK, CD31, CD45, CD68, cyclin D1, βcatenin, E-cadherin, NF-κB, and HIF-1α was analysed by immunohistochemistry (IHC) in OSCC tumor tissues and tumor distant normal tissues. Results. The 16S rDNA results showed that the detected amount of Fusobacterium in OSCC tumor tissues was significantly larger than that in tumor distant normal tissues. High expression of Fusobacterium was significantly correlated with the lifestyle-related oral risk habits, including smoking (p=0.036) and alcohol consumption (p=0.022), but did not correlate with patient sex, age, tumor laterality, tumor size, grade or TNM stage. Fusobacterium nucleatum was enriched in tumor stroma, where CD31+ blood vessels and inflammatory cells (including CD45+ leukocytes and CD68+ macrophages) were densely distributed. Cyclin D1 was mainly expressed in the nucleus of tumor cells. β-catenin was expressed in the tumor cell membrane and was positively expressed in tumor interstitial vascular endothelial cells. E-cadherin was mainly expressed in tumor cell membranes. NF-κB was positively expressed in the cytoplasm of tumor cells, tumor interstitial cells and myo-fibrocytes. HIF-1α was mainly expressed in the cytoplasm of tumor interstitial cells. HIF-1α was highly expressed where Fusobacterium nucleatum was densely distributed. Conclusion. According to our study, the detected amount of Fusobacterium in OSCC tumor tissues was significantly larger than that in tumor distant normal tissues, and Fusobacterium nucleatum might aggravate inflammation and hypoxia by interacting with NF-κB and HIF-1α in OSCC.