Browsing by Subject "Leukocyte"

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    Do adhesion molecules importantly regulate leukocyte kinetics within intraacinar microvessels of the lung.
    (Murcia : F. Hernández, 1998) Yamaguchi, K.; Nishio, K.; Aoki, T.; Suzuki, Y.; Sato, N.; Takeshita, K.; Kudo, H.
    Precise assessment of blood cell kinetics in the pulmonary microcirculation is extremely difficult because pulmonary microvascular architecture contains arterioles, venules and capillaries in an exceedingly intricate and densely convoluted fashion. Conventional epiluminescence microscopy may not be suitable for investigation of blood cell kinetics in the pulmonary microcirculation, in which arterioles, venules and capillary networks are not located in the same plane. To overcome these impediments, we recently developed a real-time confocal laser luminescence microscope with a high-speed analysis component having the capacity to yield confocal-images of rapidly moving cells at a rate of 1,000 frameslsec and at sufficiently high magnification. In the current review, we will first introduce the details of our newly developed observation system constructed with a view to estimation of blood cell dynamics in the intraacinar microcirculation of the lung. Applying this novel method to isolated perfused rat lungs, we will secondly address the issue of whether or not leukocyte-endothelium interactions in the pulmonary microcirculation qualitatively differ from those serving in the systemic microcirculation. We will particularly shed light on possible roles of endothelial ICAM-1, endothelial P-selectin and leukocyte L-selectin in distorting leukocyte kinetics in the intraacinar microvessels under a variety of diseased conditions, including prolonged exposure to a hyperoxic environment inducing a significant upregulation of ICAM-1 as well as P-selectin on the pulmonary microvascular endothelium, and stimulation of leukocytes by an IL-8 analog causing downregulation of leukocyte L-selectin but inverse upregulation of CD18-related integrins.
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    Effects of storage duration on some blood parameters of the red-eared slider trachemys scripta elegans
    (2017) Hadžimusić, Nejra; Škapur, Vedad; Hrković-Porobija, Amina; Katica, Nejla
    ABSTRACT The aim of present study was to determine the effect of storage duration and temperature on the red blood cells and white blood cells, as well as packed cell volume in blood samples from healthy adult red-eared slider. Blood samples were collected from six adult red-eared sliders. Blood samples were analyzed immediately after sampling to obtain the baseline value (BV) of red blood cells count, white blood cell count and packed cell volume. Afterwards the blood was stored at 4 ̆C and the exact hematological analyses were performed after 24h, 48h and 72h. Our research on red-eared slider blood samples showed same level of stability for RBC and WBC count during 72 hours of storage at 4°C and for PCV during 48 hours. Handling of blood samples, as well as duration of storage can significantly influence the results of hematological de-terminations. Consequently, results of hematological determinations of improperly stored or handled blood samples can yield misleading results.