Browsing by Subject "Leucocitos"

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    Effects of storage duration on some blood parameters of the red-eared slider trachemys scripta elegans
    (2017) Hadžimusić, Nejra; Škapur, Vedad; Hrković-Porobija, Amina; Katica, Nejla
    ABSTRACT The aim of present study was to determine the effect of storage duration and temperature on the red blood cells and white blood cells, as well as packed cell volume in blood samples from healthy adult red-eared slider. Blood samples were collected from six adult red-eared sliders. Blood samples were analyzed immediately after sampling to obtain the baseline value (BV) of red blood cells count, white blood cell count and packed cell volume. Afterwards the blood was stored at 4 ̆C and the exact hematological analyses were performed after 24h, 48h and 72h. Our research on red-eared slider blood samples showed same level of stability for RBC and WBC count during 72 hours of storage at 4°C and for PCV during 48 hours. Handling of blood samples, as well as duration of storage can significantly influence the results of hematological de-terminations. Consequently, results of hematological determinations of improperly stored or handled blood samples can yield misleading results.
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    Phylogeny of cytokines: molecular cloning and expression analysis of sea bass Dicentrarchus labrax interleukin-1b
    (2001) Scapigliati, G.; Buonocore, F.; Bird, S.; Zou, J.; Falasca, C.; Prugnoli, D.; Secombes, C. J.; Pelegrín Vivancos, Pablo; Bioquímica y Biología Molecular B e Inmunología
    In this paper the cloning of interleukin-1b (IL-1b) from the fish Dicentrarchus labrax (sea bass) is described. Using degenerate primers designed from known IL-1b sequences, a cDNA fragment was amplified by PCR and elongated by 3’ and 5’ RACE to give the full-length coding sequence for sea bass IL-1. The cDNA is 1292 bp, lacks a putative ICE cut site, and codes for a deduced peptide of 29.4 kDa with a pI of 5.1. Sequence analysis showed highest amino acid similarity with rainbow trout (62%), Xenopus (46%), and carp (45.5%) IL-1b sequences. Expression studies show that sea bass IL-1b can be upregulated by bacterial lipopolysaccharide both in vitro and in vivo in leucocytes from blood, head-kidney, spleen, gills and liver, whereas the IL-1b transcript was not detectable in thymus and gut-associated lymphoid tissue. Northern blot analysis with head-kidney leucocyte RNA showed a main LPS- upregulated band at 1.3 kb, and two minor bands at 0.9 and 3.0 kb, respectively. Phylogenetic comparisons with IL-1b from other vertebrates is presented.