Browsing by Subject "Lesion"

Now showing 1 - 3 of 3
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    Histopathology, microbiology and the inflammatory process associated with Sarcoptes scabiei infection in the Iberian ibex, Capra pyrenaica
    (Springer Nature, 2017-12-04) Espinosa, José; Ráez-Bravo, Arián; López-Olvera, Jorge R.; Pérez, Jesús M.; Lavín, Santiago; Tvarijonaviciute, Asta; Cano-Manuel, Francisco J.; Fandos, Paulino; Soriguer, Ramón C.; Granados, José Enrique; Romero García, Diego; Velarde, Roser; Ciencias Sociosanitarias
    Background: Sarcoptic mange has been identified as the most significant infectious disease affecting the Iberian ibex (Capra pyrenaica). Despite several studies on the effects of mange on ibex, the pathological and clinical picture derived from sarcoptic mange infestation is still poorly understood. To further knowledge of sarcoptic mange pathology, samples from ibex were evaluated from histological, microbiological and serological perspectives. Methods: Samples of skin, non-dermal tissues and blood were collected from 54 ibex (25 experimentally infected, 15 naturally infected and 14 healthy). Skin biopsies were examined at different stages of the disease for quantitative cellular, structural and vascular changes. Sixteen different non-dermal tissues of each ibex were taken for histological study. Acetylcholinesterase and serum amyloid A protein levels were evaluated from blood samples from ibex with different lesional grade. Samples of mangy skin, suppurative lesions and internal organs were characterized microbiologically by culture. Bacterial colonies were identified by a desorption/ionization time-of-flight mass spectrometry system (MALDI TOF/TOF). Results: The histological study of the skin lesions revealed serious acanthosis, hyperkeratosis, rete ridges, spongiotic oedema, serocellular and eosinophilic crusts, exocytosis foci, apoptotic cells and sebaceous gland hyperplasia. The cellular response in the dermis was consistent with type I and type IV hypersensitivity responses. The most prominent histological findings in non-dermal tissues were lymphoid hyperplasia, leukocytosis, congestion and the presence of amyloid deposits. The increase in serum concentrations of acetylcholinesterase and amyloid A protein correlated positively with the establishment of the inflammatory response in mangy skin and the presence of systemic amyloidosis. A wide variety of bacterial agents were isolated and the simultaneous presence of these in mangy skin, lymph nodes and internal organs such as lungs, liver, spleen and kidney was compatible with a septicaemic pattern of infection. Conclusions: The alteration of biomarkers of inflammation and its implication in the pathogenesis of the disease and development of lesions in non-dermal tissues and septicaemic processes are serious conditioners for the survival of the mangy ibex. This severe clinical picture could be an important factor when considering the decision to eliminate animals that exceed a certain disease threshold from a population.
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    Tetrameriosis in feral pigeons from Murcia, Southeastern Spain
    (Elsevier, 2009-08) Martínez-Carrasco Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Espigares, David; Tizzani, Paolo; Alonso, Francisco D.; Martínez Cáceres, Carlos Manuel; Sanidad Animal
    A total of 165 adult feral pigeons (Columba livia) were captured in 7 different parks of the municipality of Murcia, Southeastern Spain. Birds were evaluated clinically and subsequently necropsied. All birds appeared to be in good physical condition and no apparent signs of disease were observed. However, 17.6% of the pigeons were parasitized by Tetrameres (Petrowimeres) fissispina. The nematode burden in the proventriculus ranges from 5 to 64 specimens (median: 19); the median of female and male nematodes was 17 (range: 4–54) and 3 (range: 0–12), respectively. Despite the absence of detectable signs of disease, lesions induced by T. (P.) fissispina were evident. The histopathologic analysis revealed a pressure atrophy of the proventricular glands and a light inflammatory infiltrate surrounding the papillae. Pigeons captured in parks with soil had significantly higher T. (P.) fissispina prevalence compared with those captured in paved areas.
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    Tuberculosis caprina: Estudio lesional y su relación con el tipo de reacción diagnóstica
    (Universidad de Murcia. Servicio de publicaciones, 2022) Grincalaityte Puodziukynaite, Ramune; Buendía Marín, Antonio Julián; Párraga Ros, Ester
    En determinadas comunidades autónomas de España, existen programas de control y erradicación frente a la tuberculosis caprina para minimizar las repercusiones económicas y de salud pública. Dichos programas oficiales se fundamentan en un tipo de diagnóstico basado únicamente en inmunidad celular (prueba de intradermotuberculinización y prueba de liberación de IFN-γ), no obstante, existe un tipo de diagnóstico humoral (serología) que podría resultar útil cómo prueba adicional para detección de animales con una respuesta humoral predominante. Todas las pruebas se basan en el proceso inmunopatológico que padece el animal enfermo, por lo que resulta interesante comprender la regulación del proceso a nivel sistémico y local. En este trabajo se estudiaron 23 cabras de las cuales 10 resultaron positivas tan sólo a pruebas de inmunidad celular y otras 13 únicamente positivas a pruebas de tipo humoral. A partir de la visualización macroscópica de las lesiones, se tomaron muestras para la realización de 3 estudios: uno histopatológico mediante la clasificación microscópica de las lesiones en cerradas y abiertas, otro etiológico mediante la visualización de micobacterias con la tinción de Ziehl Neelsen y un estudio de los diferentes grupos celulares inmunológicos mediante inmunohistoquímica. El objetivo fue establecer las relaciones existentes entre el tipo de prueba diagnóstica y los distintos parámetros estudiados. Los resultados demostraron la existencia de una asociación significativa entre el diagnóstico de tipo celular, con lesiones de tipo cerrado. Asimismo, la asociación entre un diagnóstico de tipo humoral con lesiones de tipo abiertas (más infectivas), con mayor presencia de linfocitos B y linfocitos T reguladores en el área de lesión. Por tanto, se cumple nuestra hipótesis de que dichas células se hallan en un número más elevado en fases más avanzadas de la enfermedad con procesos de inflamación crónica, lo que influye en el diagnóstico condicionado por el tipo de prueba empleada.