Browsing by Subject "Leptospira"

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    Canine distemper virus may affect European wild cat populations in Central Spain
    (Elsevier GmbH, 2019-05-06) Candela, Mónica G.; Pardavila, Xosé; Lamosa, Adrián; Mangas, Julián G.; Martinez-Carrasco, Carlos; Ortega Hernández, Nieves; Sanidad Animal; Sorex Ecoloxía e Medio Ambiente S. L, Spain; Ecology Area, Department of Biology and Geology, Physics and Inorganic Chemistry, University Rey Juan Carlos, Madrid, Spain
    The main objective of this brief communication is to inform about the exposure to certain pathogens of interest for mesocarnivores in wildcats (Felis silvestris silvestris) that inhabit a human-domestic-wild ecotone located in a Natural Park (Serranía de Cuenca, Central Spain). Blood and mucosal swabs (nasal, conjunctival and rectal) samples were collected from nine alive animals to detect canine distemper virus (CDV), parvovirus (CPV/FPV), feline leukaemia virus (FeLV), feline coronavirus (FCoV), feline immunodeficiency virus (FIV), Leptospira interrogans, Chlamydia felis, Ehrlichia canis, Toxoplasma gondii, and Neospora caninum. ELISA, immunochromatograpy, microscopy agglutination test and PCR assays were used. The results show the first worldwide detection of exposure of wildcats to L. interrogans (3 positive/9 analysed) and the first detection of exposure to CVD (7/9), of carriers of C. felis (2/9) and of fecal spreading of CPV-FPV (2/9) in wildcats in Spain. Exposure to T. gondii and CPV-FPV was detected in 5 of the 9 wildcats analysed, and to FelV in 4 of 9. No FIV, FCoV, Ehrlichia canis and Neospora caninum were detected. The results reveal the circulation of pathogens among the wildcat population studied, but more vigilance is needed for an accurate assessment of the impact of these pathogens on the health status of this population.
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    Monitoring of pathogenic Leptospira infection in wolves (Canis lupus) from Spain and Italy
    (Elsevier, 2024-08-15) Cano Terriza, David; Moroni, Bárbara; Fayos, Manena; Martínez, Remigio; Oleaga, Alvaro; Robetto, Serena; Remesar, Susana; Orusa, Ricardo; Muñoz Hernández, Clara; Velarde, Roser; García Bocanegra, Ignacio; Gonzálvez Juan, Moisés; Sin departamento asociado
    Leptospirosis is a bacterial disease of worldwide distribution with relevant implications for animal and human health. Different large wild carnivore species can act as reservoirs of this zoonotic pathogen. This study aimed to evaluate the circulation of Leptospira spp. in free-ranging wolves (Canis lupus) from southern Europe. A total of 281 kidney samples of wolves from Spain and Italy were collected between 2017 and 2023. The presence of Leptospira DNA was analysed by real-time PCR and phylogenetic analyses were carried out using a Bayesian approach. The overall prevalence was 3.2 % (9/281; 95 %CI: 1.1–5.3). Leptospira DNA was detected in nine of the 180 wolves from Spain (5.0 %; 95 %CI: 1.8–8.2), but not in the Italian wolf population (0 %; 0/101). Molecular analyses revealed high homology between the sequences obtained in the present study and isolates of Leptospira interrogans and Leptospira borgpetersenii from different rodent and domestic ungulate species. Our results provide evidence of a low and spatially heterogeneous circulation of this pathogen in wolf populations of southern Europe. The detection of zoonotic Leptospira species in this survey supports the need to consider wolf populations in monitoring programs for leptospirosis with a One Health approach.