Browsing by Subject "Isolation"

Now showing 1 - 4 of 4
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    A Size-Exclusion Chromatography-Based Procedure for Isolating Extracellular Vesicle Subsets from Porcine Seminal Plasma
    (Humana Press, 2024-04-10) Martínez Díaz, Pablo; Ana Parra; Christian M Sanchez-López; Antonio Marcilla; Bucci, Diego; Roca Aleu, Jorge; Barranco Cascales, Isabel; Medicina y Cirugía Animal; Facultad de Veterinaria
    Extracellular vesicles (EVs), membrane nanoparticles (30-to-1000 nm diameter) secreted and released by most of the body functional cells, have emerged as powerful cell-to-cell messengers transferring their bioactive cargo (proteins, lipids, and nucleic acids) from donor to recipient cells. The promising potential utility of EVs as both noninvasive biomarkers and therapeutic carriers for several pathologies, including some types of cancers, has attracted increasing scientific interest. EVs can be found in all body biofluids, including seminal plasma, a complex fluid consisting mainly of a mixture of secretions of the epididymis and accessory sex glands. Seminal EVs are involved in modulating both sperm physiological processes and immune environment of the internal female genital tract, thus playing an essential indirect role in fertilization and embryo development. Seminal plasma, alike other biofluids, contains a heterogenous population of EV-subsets. However, the lack of consensus on the most accurate procedure for isolating EV-subsets has led to a poor definition of their composition/function. Currently, size exclusion chromatography (SEC), a size-selective separation method, is one of the most promising EV-isolation procedures, allowing the isolation of EVs from biological fluids in a purer, easier, cheaper, and more scalable way compared to other alternative isolation procedures. This chapter reports a SEC-based protocol, combined with differential centrifugation and ultrafiltration, to isolate two subsets of seminal EVs differing in size (large and small EVs) in the ejaculate of pigs, a livestock species of great productive interest and an outstanding animal model for human reproduction.
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    Las enseñanzas híbridas (blended learning) ante el aislamiento del estudiantado: lecciones de la literatura
    (Universidad de Murcia, Servicio de Publicaciones, 2026-01-01) Sevilla Sevilla, Claudia; Jerez Jerez, María Jesús; Bujidos Casado, María; Navío Marco, Julio; Sin departamento asociado
    Distance education can lead to feelings of isolation, disconnection and depersonalisation on the part of the learner. This phenomenon has begun to be studied especially in online learning, and to a much lesser extent in blended learning. The pandemic has also exacerbated this sense of isolation, so it is timely to consider whether studies on blended learning are addressing student isolation, and what lessons previous research can teach us. A review of the literature and a cluster analysis shows that the sense of isolation resulting from the pandemic somewhat hides the isolation that blended methodology can provoke. Isolation is still attributed to the online part of the hybrid teaching and especially to the asynchronous part, but little is said in the literature about blended learning as a whole. There is a lack of analysis that comprehensively assess this phenomenon, studying the appropriate design of hybrid courses to address isolation holistically. This study itself makes some recommendations and suggestions for further work to lessen the isolation that students may feel in blended learning, and in distance learning in general.
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    Liquid biopsy of circulating tumor cells: From isolation, enrichment, and genome sequencing to clinical applications
    (Universidad de Murcia, Departamento de Histología e Histopatología, 2025) Tan Keqin; Zhu Hong; Ma Xuelei; Biología Celular e Histología
    Circulating tumor cells (CTCs), shed from primary tumors into the bloodstream, play a crucial role in metastasis and hold great potential in cancer diagnosis, prognosis, and treatment monitoring. Conventional CTC detection using epithelial biomarkers like epithelial cell adhesion molecule (EpCAM) for immunocapture overlooks mesenchymal-like CTCs with high metastatic potential, spurring the development of non-immunocapture technologies that use biophysical traits for enrichment. Innovations in microfluidic platforms and multi-parametric sorting improve isolation efficiency and address related challenges. Breakthroughs in single-cell genomic and transcriptomic sequencing enable in-depth molecular characterization of CTCs. Clinically, CTC enumeration and molecular profiling are emerging as real-time tools for assessing therapeutic response and predicting outcomes, especially in metastatic breast, prostate, and colorectal cancers. This review focuses on CTC isolation, enrichment techniques, their applications in different tumors, downstream analysis progress, and potential in precision medicine
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    Personas mayores, participación y cambio social antes y después del COVID-19
    (Universidad de Murcia, Servicio de Publicaciones, 2021) Medina Ruiz, Elvira
    La participación social está estrechamente ligada al bienestar y un envejecimiento saludable generando beneficios físicos y psicológicos. Sin embargo, las personas mayores tienen tendencia a bajar los niveles de participación e interacción social tras la jubilación, aunque otros muchos continúan militando y participando en entidades y movimientos sociales. La pandemia provocada por la COVID-19 ha tenido un fuerte impacto a nivel social y sanitario siendo los principales perjudicados las personas mayores, no solo por las elevadas tasas de mortalidad sino porque las medidas de distanciamiento social impuestas para su protección, han dado lugar a un agravamiento del aislamiento social y el sentimiento de soledad de nuestros mayores. Es urgente generar espacios seguros para aumentar los niveles de participación social.