Browsing by Subject "Ischemic stroke"

Now showing 1 - 4 of 4
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    Electroacupuncture pretreatment inhibits ferroptosis and inflammation after middle cerebral artery occlusion in rats by activating Nrf2
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Pu, Yanpeng; Cheng, Jingyan; Wang, Zhenya; Zhang, Jingbo; Liang, Fajun; Zhang, Xianbao; Zheng, Zhijun; Yin, Miaomiao; Wang, Zhen
    Objective. Electroacupuncture (EA) pretreatment can effectively increase the tolerance of the brain to ischemic stroke. The mechanism of ischemic tolerance induced by EA is related to Nrf2, but its specific mechanism has not been elucidated. This paper was designed to explore the effect of EA pretreatment on brain injury and the related mechanisms. Methods. Rats were pretreated with EA before middle cerebral artery occlusion (MCAO) modeling. The symptoms of neurological deficit and the volume of cerebral infarction were measured. The levels of inflammatory factors, oxidative stress-related factors, LPO, ROS, and Fe2+ were evaluated by the corresponding kits. Cell apoptosis was determined through TUNEL staining. The mRNA expression of inflammatory factors was examined by RT-qPCR, and the protein expression of ferroptosis-related factors, pyroptosis-related proteins, Keap1, Nrf2, HO-1, and NQO1 by western blotting. Results. EA pretreatment improved the symptoms of neurological deficit and reduced the volume of cerebral infarction. EA pretreatment significantly inhibited oxidative stress, inflammatory response, ferroptosis, pyroptosis, and apoptosis in brain tissues of MCAO rats. Mechanistically, EA pretreatment could activate Nrf2 expression and reduce Keap1 expression. Conclusion. EA pretreatment reduced inflammation and oxidative stress and inhibited ferroptosis by activating Nrf2 expression, ultimately delaying the development of ischemic stroke.
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    Leptomeninges: a novel stem cell niche harboring ischemia-induced neural progenitors
    (F. Hernández y Juan F. Madrid. Universidad de Murcia: Departamento de Biología Celular e Histología, 2015) Nakagomi, Takayuki; Nakano-Doi, Akiko; Matsuyama, Tomoiro
    It is well known that neural stem cells (NSCs) are present in many parts of the central nervous system (CNS), including the subventricular zone (SVZ) of the lateral ventricle, subgranular zone (SGZ) of the hippocampal dentate gyrus, cortex, and spinal cord. Using a mouse model of cortical infarction, we demonstrated for the first time that NSCs, which can differentiate into neural lineage cells, could be induced in the meninges (leptomeninges) of ischemic brain areas as well. However, such ischemia-induced NSCs (iNSCs) were not observed in the leptomeninges of non-ischemic areas. This suggests the leptomeninges, which surround the CNS, might be a novel stem cell niche harboring endogenous iNSCs following brain injury. In this review, we introduce the characterization and possible origin of leptomeningeal iNSCs based on our reports and recent findings. We also refer to the potential of leptomeningeal iNSCs for cortical neurogenesis.
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    Research on the Cognitive Orientation to daily Occupational Performance Approach: a bibliometric review
    (American Occupational Therapy Association, 2024-10-25) Valera Gran, Desirée; Delgado Lobete, Laura; Montes Montes, Rebeca; Navarrete Muñoz, Eva María; Atención Sociosanitaria; Facultades de la UMU::Facultad de Ciencias Sociosanitarias (Lorca)
    Importance: The Cognitive Orientation to daily Occupational Performance (CO-OP) is an evidence-based intervention approach that has significantly increased in popularity over the past two decades. However, how the research literature on this topic is patterned is still unknown, so it is difficult to identify potential areas for research and clinical interest. Aims: To analyze the literature published on the CO-OP approach to provide a detailed and structured analysis of the publication patterns. Data Sources: All literature related to CO-OP included in the Web of Science database through June 15, 2024. Study Selection and Data Collection: All published studies related to CO-OP were included in the bibliometric analysis, which was conducted on the raw data retrieved from the Web of Science database using the Bibliometrix R package. Findings: The annual growth rate in CO-OP research has been 0.8%, significantly increasing from 2015 onward. Most of the research has been published in occupational therapy and rehabilitation journals by English-speaking research teams, and its scope has expanded from developmental coordination disorder to a wide range of health conditions. In addition, two main lines of research have emerged: one focusing on understanding the underlying cognitive processes involved in CO-OP and the other focusing on its clinical effectiveness. Conclusions and Relevance: Research on CO-OP has significantly increased over the past decade, and it currently encompasses a wide range of areas. This analysis may facilitate the advancement of research on and the clinical practical application of CO-OP. Plain-Language Summary: The Cognitive Orientation to daily Occupational Performance (CO-OP) is an evidence-based intervention approach that has significantly increased in popularity over the past two decades. Occupational therapists use it to improve the occupational performance of both children and adults with movement difficulties. However, it is unknown how the research literature on this topic is patterned, so it is difficult to identify potential areas for research and clinical interest. This study found that scientific literature on CO-OP has significantly increased in the past decade and that its scope has expanded from developmental coordination disorder to a wider range of health conditions, such as cerebral palsy. Moreover, research is now focused on two main questions: What are the underlying cognitive processes involved during intervention, and how effective is the CO-OP approach? These findings can be used to further improve occupational performance and participation among occupational therapy clients who struggle with motor performance and planning.
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    Validación y valoración del software eASPECTS de análisis automático de imagen en TC en el ictus agudo : capacidad diagnóstica y pronóstica
    (Universidad de Murcia, 2025-11-11) Zamarro Parra, Joaquín; Parrilla Reverter, Guillermo; Berná Mestre, Juan de Dios; Sin departamento asociado; Escuela Internacional de Doctorado
    Introducción. El ictus isquémico agudo es una de las principales causas de discapacidad y mortalidad. La valoración urgente por imagen, basada en tomografía computarizada sin contraste (NCCT), emplea ASPECTS para detectar cambios isquémicos precoces, pero su lectura manual presenta variabilidad. e-ASPECTS automatiza el cálculo de ASPECTS y estima el volumen del núcleo isquémico (eAIV), aportando métricas reproducibles que requieren validación frente a lectura experta y desenlaces clínicos. Objetivos. Evaluar la capacidad diagnóstica y pronóstica de e-ASPECTS en pacientes con oclusión de gran vaso de circulación anterior tratados con trombectomía, así como analizar si la combinación de e-ASPECTS, eAIV y el estado de la circulación colateral mejora la predicción del desenlace clínico. Métodos: Estudio retrospectivo, cohorte consecutiva. Se registraron variables clínicas y de imagen. NCCT basal se interpretó de forma manual (ASPECTS) y automática (e-ASPECTS y eAIV). La circulación colateral se evaluó manual (CTA, dicotómica >50 % vs ≤50 %) y automática con e-Stroke Suite: e-CTA-CS (ordinal 0–3) y e-CTA% (índice continuo). El desenlace fue mRS a 90 días. Se emplearon correlaciones, AUC y regresión logística. Resultados: Se incluyeron 146 pacientes con edad 66,5 ± 12,6 años, varón 52,0 % y NIHSS 17 [13–21,25]). ASPECTS manual y e-ASPECTS (medianas 8 [7–9] y 8 [6–9]) mostraron alta correlación (r = 0,82; p < 0,001). El AUC para e-ASPECTS fue 0,692 (IC 95 %: 0,61–0,77; p < 0,001) y para eAIV 0,716 (IC 95 %: 0,62–0,82; p = 0,002) en la predicción de mRS 0–2. En el modelo multivariante, e-ASPECTS (OR 1,65 por punto; IC 95 %: 1,32–2,05; p < 0,001) y eAIV (OR 0,97 por mL; IC 95 %: 0,95–0,99; p = 0,002) fueron predictores independientes. En 134 de los 146 casos, se encontró una buena concordancia (índice k: 0.62, IC 0.58-0.65) entre la evaluación manual y automática (e-CTA-CS y e-CTA%) de la circulación colateral (p<0,05) y se encontró una asociación entre estado de colaterales abundante y desenlace con independencia funcional (p=0,002). al. El score combinado (e-ASPECTS ≤ 7, eAIV ≥ 50 mL, CTA-CS pobre) evidenció un descenso escalonado de independencia funcional (57,7 %, 37,9 %, 33,3 % y 4,0 % para 0, 1, 2 y 3 factores). Conclusiones: e-ASPECTS del NCCT basal presenta una alta capacidad diagnóstica y pronóstica en pacientes con ictus isquémico agudo, y junto con eAIV predice de forma independiente el pronóstico funcional. La integración de e-ASPECTS, eAIV y colaterales mejora la estratificación frente a métricas aisladas. Estos hallazgos respaldan la incorporación de herramientas automáticas en rutas de ictus, democratizando decisiones basadas en tejido con NCCT ± CTA, incluso en entornos con recursos limitados.