Browsing by Subject "Infrared spectroscopy"

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    The Influence of a membrane environment on the structure and stability of a prokaryotic potassium channel, KcsA
    (Elsevier, 2005-09-26) Encinar Hidalgo, José Antonio; Molina Gallego, María Luisa; Poveda Larrosa, José Antonio; Barrera Olivares, Francisco Nicolás; Renart Pérez, María Lourdes; Fernández Carvajal, Asia María; González Ros, José Manuel; Bioquímica y Biología Molecular B e Inmunología
    The lack of a membrane environment in membrane protein crystals is considered one of the major limiting factors to fully imply X-ray structural data to explain functional properties of ion channels [Gulbis, J.M. and Doyle, D. (2004) Curr. Opin. Struct. Biol. 14, 440–446]. Here, we provide infrared spectroscopic evidence that the structure and stability of the potassium channel KcsA and its chymotryptic derivative 1–125 KcsA reconstituted into native-like membranes differ from those exhibited by these proteins in detergent solution, the latter taken as an approximation of the mixed detergent-protein crystal conditions.