Browsing by Subject "Immunomorphology"

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    Integrins in ageing cartilage tissue in vitro
    (Murcia : F. Hernández, 1993) Shakibaei, M.; Abou-Fiebye, H.; Merker, H. J.
    Matrix-cell interactions are of great irnportance for numerous cell functions whereby integrins play an essential role as transmicters of extracellular signals. In cultures of ageing cartilage tissue (organoid or high density cultures) cartilage cells occur on the surface of which thick fibrils of collagen type 1 are deposited. Since integrins, in their role as receptors, cause an interaction between matrix components and cell membrane, we tried to demonstrate immunomorphologically (light and electron microscopically) the corresponding integrin receptors for collagen type 1 (B, al and B1%) on the surface of these ageing cartilage cells. Cultures of normal, i.e. young cartilage tissue exhibit only Ola3- and Bla5~receptorsl;a belling against the integrins Blal and Bla2 1s not possible in this case. Our results show that after the occurrence of thick fibrils cartilage cells express new receptors (Blal and fila2)o n the cell membrane. Thus, in ageing or dedifferentiating cartilage tissue it is not only the synthesis programme of matrix components (e.g. instead of collagen type 11 >> collagen type 1) which changes but also the integrins (instead of a3/B1, a51Bl >> allB1, a2/B1) so that new collagen types can be bound. These findings may also serve for a better understanding and interpretation of cartilage changes in vivo during ageing and under pathological conditions.
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    lmmunomorphologica characteristics of renal cell carcinoma
    (Murcia : F. Hernández, 1995) Markovic-Lipkovski, J.; Brasanac, D.; Todorovic, V.; Múller, Gerhard Anton; Múller, Gerhard Anton
    Immunomorphological characteristics of 27 renal cell carcinoma (RCC): 18 clear cell, 6 granular (chromophilic), 2 chromophobe, 1 spindle cell (sarcomatoid) as well as of 1 oncocytoma, were analyzed. The investigation was performed on cryostat sections by immunoperoxidase technique applying a panel of monoclonal antibodies which defined: proximal (TNE3, TN5, 5D9) and distal (TN8, TN9, 7C2) tubular antigens; intercellular adhesion molecule 1 (ICAMl); HLA class I1 (-DQ, -DR and -DP) antigens, intermediary filaments (cytokeratin and vimentin); and antigens on tumour infiltrating mononuclear leucocytes (TT1, TT2 and LeuM3 for CD4, CD8 and CD14 antigens, respectively). All RCC with exception of chromophobe CO-expressed cytokeratin and vimentin. In addition, they were usually positive for all proximal and two distal tubular markers (TN8, TN9) indicating primitive cells which could differentiate into the epithelium of both parts of tubule system as the most probable originators of in RCC. Almost all RCC but the chromophobe aberrantly expressed HLA class I1 antigens which great variability from case to caie. The presence of HLA-DR antigens was more intensive and widespread than of HLA-DQ and-DP antigens. Expression of ICAMl mostly correlated with presence of HLA class I1 antigens, particularly with -DR on tumour cells of RCC HLA-DR antigen expression was always more prominent than mononuclear cell infiltrate (among which macrophages prevailed over T cells) which could suggest that increased histocompatibility antigen expression precedes mononuclear cell influx. In contrast to all other RCC, chromophobe tumours had quite distinct features revealing the most intense reaction with 7C2 (MAb that produced the weakest reaction with other tumour types), absence of vimentin and very weak reaction with antibodies for HLA class Il Ag and ICAM 1. Since oncocytoma has similar immunohistological properties it could be supposed that both tumours have common histogenesis.