Browsing by Subject "Immunology"
Now showing 1 - 7 of 7
Results Per Page
Sort Options
PublicationOpen AccessArchivos de datos en formato Flow Cytometry Standard (FCS) para analizar con software de citometría de flujo(2017-01-24) Rubio Pedraza, Gonzalo; Bioquímica y Biología Molecular "B" e Inmunología; Facultad de Química- PublicationRestrictedArtemisia pollen is the main vector for airborne endotoxin(Elsevier, 2018-08-09) Oteros, José; Bartusel, Elke; Alessandrini, Francesca; Núñez, Andrés; Moreno, Diego A.; Behrendt, Heidrun; Schmidt-Weber, Carsten; Traidl-Hoffmann, Claudia; Buters, Jeroen; Genética y MicrobiologíaBackground: Endotoxin (LPS) released from gram-negative bacteria causes strong immunologic and inflammatory effects and, when airborne, can contribute to respiratory conditions, such as allergic asthma. Objectives: We sought to identify the source of airborne endotoxin and the effect of this endotoxin on allergic sensitization. Methods: We determined LPS levels in outdoor air on a daily basis for 4 consecutive years in Munich (Germany) and Davos (Switzerland). Air was sampled as particulate matter (PM) greater than 10 μm (PM > 10) and PM between 2.5 and 10 μm. LPS levels were determined by using the recombinant Factor C assay. Results: More than 60% of the annual endotoxin exposure was detected in the PM > 10 fraction, showing that bacteria do not aerosolize as independent units or aggregates but adhered to large particles. In Munich 70% of annual exposure was detected between June 12th and August 28th. Multivariate modeling showed that endotoxin levels could be explained by phenological parameters (ie, plant growth). Indeed, days with high airborne endotoxin levels correlated well with the amount of Artemisia pollen in the air. Pollen collected from plants across Europe (100 locations) showed that the highest levels of endotoxin were detected on Artemisia vulgaris (mugwort) pollen, with little on other pollen. Microbiome analysis showed that LPS concentrations on mugwort pollen were related to the presence of Pseudomonas species and Pantoea species communities. In a mouse model of allergic disease, the presence of LPS on mugwort pollen was needed for allergic sensitization. Conclusions: The majority of airborne endotoxin stems from bacteria dispersed with pollen of only one plant: mugwort. This LPS was essential for inducing inflammation of the lung and allergic sensitization.
- PublicationOpen AccessCruzando el cuerpo. Dispositivos de frontera y procesos de subjetivación(Universidad de Murcia. Servicio de Publicaciones, 2016) Domenech de la Lastra, PabloEn este texto buscamos la posibilidad de comprender al cuerpo del migrante como un límite político y ontológico a la construcción de dispositivos fronterizos contemporáneos, centrándonos fundamentalmente en la frontera sur de Europa. Primeramente, daremos cuenta de la emergencia ontológica del migrante como objeto-cuerpo por el poder soberano. Posteriormente, bosquejaremos las formas de aprehensión de este objeto-cuerpo por los poderes gubernamentales, que construyen nuevas formas de sujeción-subjetivación. Finalmente, apuntaremos algunas formas de desbordamiento de los dispositivos fronterizos, para poder hablar de la aparición de un sujeto emancipado, que ejerce de fuerza límite contra las dinámicas globales de vallado.
- PublicationOpen AccessExperiencias de Docencia Virtual en Facultades de Medicina Españolas durante la pandemia COVID-19 (II): Farmacología, Inmunología(Centro de Estudios en Educación Médica de la Universidad de Murcia (CeuEM) y Editum (Servicio de Publicaciones de la Universidad de Murcia), 2020) Sanz Alvarez, Emilio; Vicente Romero, Jorge; Prieto Martín, AlfredoPresentamos un resumen de las actividades que algunos profesores deFacultades de Medicina españolas han llevado a cabo durante las 3 semanas previasa las vacaciones de primavera. Durante este tiempo, debido a la pandemia provocadapor la COVID-19, la docencia presencial tuvo que ser sustituída por actividades enlínea o virtuales, a causa de la implantación del estado de alarma en España quemotivó el cierre completo de las Universidades desde el 13 de marzo de 2020. Lasexperiencias son de Farmacología y de Inmunología.
- PublicationOpen AccessImmunology of tissue homeostasis, ovarian cancer growth and regression, and long lasting cancer immune prophylaxis - review of literature(Universidad de Murcia. Departamento de Biología Celular e Histología, 2021) Bukovsky, AntoninData on the substantial physiological role of the immune system in the organism’s ability to manage proper differentiation and function of normal tissues (tissue homeostasis), and detailed causes of the immune system’s essential role for the in vivo stimulation of cancer growth, are severely lacking. This results in a lack of effective cancer immunotherapy without adverse events, and in the lack of long-lasting cancer immune prophylaxes, particularly in ovarian cancers. Elimination of blood auto-antibodies blocking anti-cancer T cell effectors by intermittent moderate doses of cyclophosphamide, facilitation of the immune system reactivity against alloantigens of cancer cells by two subsequent blood transfusions, and augmentation of anticancer immunity by weekly intradermal injections of bacterial toxins, caused during the subsequent treatmentfree period, lasting for two to four weeks, regression of inoperable epithelial ovarian cancers and regeneration of the tremendously metastatically altered abdominal tissues into normal healthy conditions without multivisceral cytoreductive surgery, which can result in life-threatening consequences. An otherwise untreated rectal cancer, progressing over 3 years, regressed after severe toxic dermatitis lasting over one week. This was caused by an accidental consumption of a large raw shiitake mushroom. Subsequent daily consumptions of 2 g Metformin ER and honeybee propolis ethanol extract, and weekly single larger raw shiitake mushroom, which all stimulate immune system reactivity against cancer stem cells, prevented malignant recurrence over the next 29 years without recurring dermatitis, and maintained healthy organism’s conditions. These observations indicate that regression of advanced inoperable cancers and long-lasting cancer immune prophylaxis can be reached by simple approaches
- PublicationOpen AccessManual de prácticas de laboratorio en Inmunología Clínica. 2ª Edición. G. Rubio(2017-01-26) Rubio Pedraza, Gonzalo; Bioquímica y Biología Molecular "B" e Inmunología; Facultad de Química
- PublicationOpen AccessManual de software flowing para microaula de citometría de flujo(2017-01-24) Rubio Pedraza, Gonzalo; Bioquímica y Biología Molecular "B" e Inmunología; Facultad de Química