Browsing by Subject "IRS-4"

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    Identification of new tissue markers for the monitoring and standardization of penile cancer according to the degree of differentiation
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2025) Casanova Martín, Carlos; Liviu Boaru, Diego; Fraile Martínez, Oscar; García Montero, Cielo; Leon Oliva, Diego De; Castro Martinez, Patricia De; Gimeno Longas, Maria José; Buján, Julia; García Honduvilla, Natalio; Guijarro, Luis G; Gragera, Raquel; Saez, Miguel A.; Ferrara Coppola, Connie; Baena Romero, Víctor; Diaz Pedrero, Raul; Alvarez Mon, Melchor; Toledo Lobo, M. Val; Ortega, Miguel A.; López González, Laura; Biología Celular e Histología
    Penile cancer is an uncommon disease compared with other urological tumors and is more common in low- and middle-income countries. Risk factors include age, ethnicity, smoking, hygiene, and human papillomavirus infection. Although carcinoma of the penis can be cured in up to 80% of cases if detected early, late diagnosis drastically reduces survival rates, especially in metastatic cases. More than 95% of cases are squamous cell carcinomas, and the degree of cell differentiation is a key histopathological factor, distinguishing between poorly (P), moderately (M), and well-differentiated (W) carcinomas, with verrucous carcinoma (V) having the best prognosis due to its low metastatic capacity. This study analyses the differential expression of several biomarkers related to cell proliferation and cell cycle, inflammation, epigenetics, and autophagy (cell cycle (IRS-4, Ki-67, RB1, CDK4, cyclin D1, ERBB2, β-catenin, and MAGE-A), inflammation (COX2, NLRP3, and AIF-1), epigenetics (HAT-1) and autophagy (ULK-1 and ATG9A) in penile carcinoma according to the degree of differentiation. Immunohistochemical techniques were performed on 34 penile squamous cell carcinoma (PSCC) samples classified into subtype V (N=6), and groups P (N=9), M (N=9), and W (N=10). The findings suggest a differential expression of molecules according to the degree of cell differentiation, with a higher differential expression of molecules according to the degree of cell differentiation, suggesting that the proteins studied could have predictive value. The study highlights the complexity of PSCC and the need for future studies to explore translational applications and search for new biomarkers to improve clinical management and understanding of this disease
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    Prognostic role of IRS-4 in the survival of patients with pancreatic cancer
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2022) Ortega, Miguel A; Pekarek, Leonel; Garcia Montero, Cielo; Fraile Martinez, Oscar; Saez, Miguel A; Asúnsolo, Angel; Alvarez Mon, Miguel A; Monserrat, Jorge; Coca, Santiago; Toledo Lobo, M. Val; García Honduvilla, Natalio; Albillos, Agustin; Buján, Julia; Alvarez Mon, Melchor; Guijarro, Luis G
    Pancreatic cancer is a malignancy of rising incidence, especially in developed countries due to causes such as sedentary lifestyles, tobacco smoking and ultraprocessed high fat and high sugar diets, amongst others. It is in fact the 7th cause of cancer-related deaths worldwide, and, in the following years, it is expected to climb upwards to 2nd position, after lung cancer. This is because it may have an asymptomatic course, and when it becomes evident it is in advanced stages, accompanied by metastasis generally. For this reason, survival rates are so low and, even in the few successful cases there is a high possibility of recurrence. Identifying new molecular biomarkers is arising as a highly useful tool for pancreatic cancer clinical management, although much research and work remain to be done in this field. Thus, the present study aims to analyze a series of molecules (IRS-4, Rb1, Ki-67 y COX-2) as candidates for prognosis and survival by immunohistochemistry techniques. Additionally, a 60-month longitudinal surveillance program was conducted, associated with diverse clinical parameters. Kaplan-Meier curves estimating the time of survival according to tumoral expression of those molecules denoted a low cumulative survival rate. Importantly, we observed that high levels of IRS-4 were significantly associated with a bad prognosis of the disease, increasing 160 times the mortality risk. In this way, our research showed a relevant value of these biomarkers in pancreatic cancer patients’ survival, opening a pathway for future research areas designed to inhibit these components