Browsing by Subject "Goats"

Now showing 1 - 4 of 4
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    Cephalosporin susceptibility of Staphylococcus aureus strains isolated from commercial rabbit and goat farms in Spain
    (Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise 'G. Caporale', 2022-12-31) Badillo Puerta, Elena; Escudero, Elisa; Galecio, Juan Sebastian; Marín, Pedro; Farmacología
    Antimicrobial drug resistance is an important problem that challenges veterinary clinicians to provide effective treatments without further spreading this resistance to other animals and people. The most commonly used pharmacodynamic parameter to define potency of antimicrobial drugs is minimum inhibitory concentration (MIC). The aim of this study was to evaluate the antibiotic susceptibility of thirty-six strains of Staphylococcus aureus isolated from dairy goats with mastitis and rabbits with chronic staphylococcosis. Four cephalosporins were tested: cephalexin, cephalotin, cefonicid and ceftiofur. MIC tests were performed according to the microdilution broth method. The calculated values of sensitivity in goats and rabbits were 66.67% and 72.22% for cephalexin, 72.22 % and 94.44% for cefonicid, 77.78% and 94.44% for cephalotin and 77.78% and 100% for ceftiofur, respectively. For all antibiotics, MIC90 of S. aureus from rabbits were lower than MIC90 from goats. These data suggest that more antibiotics are used in goat milk production than in rabbit farming. According to MIC values obtained in this study, ceftiofur and cephalotin may be the best option for treating S. aureus infections in lactating goats. For rabbits, ceftiofur showed lowest MIC values, but cephalosporins can produce fatal diarrhoea in this species, therefore additional studies are needed to evaluate the effects of repeated ceftiofur administration on microflora of rabbits before recommending the use of this antibiotic in this species.
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    Isolation of Mycoplasma auris from milk of goats with clinical mastitis
    (Elsevier, 2020-03-07) García-Galán Pérez, Ana; García Romero, Edgar; Sánchez López, Antonio; Corrales Romero, Juan Carlos; Contreras de Vera, Antonio; Fé Rodríguez, David Christian de la; Sanidad Animal; Facultades de la UMU::Facultad de Veterinaria
    The unexpected presence of Mycoplasma auris was evidenced in mastitis samples collected from a dairy goat herd under surveillance programmes of contagious agalactia (CA), a serious disease affecting Mediterranean small ruminant herds. Thus, control programs based on permanent analysis of milk from bulk tank and clinical mastitis, as well as ear swabs, are currently conducted in affected areas. In order to analyze the presence of CAcausing species, three, 11 and 18 of these samples were respectively collected from the same goat herd. Mycoplasmas were isolated in four milk samples from clinical mastitis and two ear swabs samples and no other pathogen was detected. The presence of CA-causing mycoplasmas was discarded by PCR. However, M. auris was identified in all the positive samples after the amplification and posterior PCR product sequencing of a partial fragment of their 16S-ribosomal ARN gene. As far as we know, these are the first isolations of M. auris from milk of goats with clinical mastitis.
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    Metabarcoding analysis of the microbiota in flocks naturally infected by Coxiella burnetii: First description of the global microbiota in domestic small ruminants
    (Elsevier, 2025-06) Contreras de Vera, Antonio; Sanidad Animal
    This study investigates Q fever in sheep and goats, key reservoirs for human infection, by metabarcoding and comparing it with q-PCR and serology. Samples from 26 small ruminant (aborted and normal-delivery) and six males across three Q fever-affected herds were analyzed. In sheep herds, seropositivity was 50 and 80% respectively, with Coxiella (C.) burnetii shedding detected vaginally in the second herd. In goats, 100% seropositivity and 90% C. burnetii detection were observed, with nasal and vaginal samples showing the highest detection rates. Metabarcoding revealed significant differences in alpha diversity, with greater richness in blood and evenness in milk from normal-delivery sheep and higher evenness in faeces from aborted sheep. Beta diversity showed richer and distinct vaginal microbiota in normal-delivery females compared to aborted ones. Firmicutes was the most abundant phylum observed.Dominant genera included: Moraxella (nasal); Mycoplasma, (blood); Streptococcus (milk); Ureaplasma (vaginal and preputial); Rikenellaceae RC9 gut group (faeces). Significant differences in bacterial composition, including infertility-linked vaginal pathogens, were found across female’s groups in all herds in the anatomical locations studied, revealing new species and tropisms. Moreover, taxonomic analysis identified C. burnetii in vaginal, milk and environmental samples. This first report of C. burnetii in the caprine nasal cavity suggests an underestimated tropism and may improve Q fever diagnosis. These findings underscore the need for herd-wide Q fever control measures, including males and normal-delivery females. Our findings contribute to new insights into the pathogen’s impact on small ruminant microbiota and a novel approach to studying infectious diseases in this sector.
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    Susceptibility of caprine mastitis pathogens to tildipirosin, gamithromycin, oxytetracycline, and danofloxacin: effect of serum on the in vitro potency of current macrolides
    (Springer, 2022-09-13) Galecio Naranjo, Juan Sebastian; Escudero Pastor, Elisa; Corrales, Juan Carlos; García-Romero, Edgar; Fe, Christian de la; Hernandis Belenguer, Verónica; Marín Carrillo, Pedro; Farmacología
    Mastitis is a significant disease in dairy ruminants, causing economic losses to the livestock industry and severe risks to public health. Antibiotic therapy is one of the most crucial practices to treat mastitis, although the susceptibility of caprine mastitis pathogens to current antibiotics has not been tested under standard or modified incubation conditions. This work evaluated the in vitro activity of tildipirosin, gamithromycin, oxytetracycline, and danofloxacin against caprine mastitis pathogens incubated following standard conditions of Clinical and Laboratory Standards Institute (CLSI) and deviation method by 25% supplementation with goat serum. Mycoplasma agalactiae, Escherichia coli, Staphylococcus aureus, Streptococcus spp., and coagulase-negative Staphylococci (CNS) were isolated from dairy goats with mastitis in Spain. Minimum inhibitory concentrations (MICs) were determined using the broth microdilution technique. The lowest MIC90 under standard conditions was obtained with danofloxacin for mastitis-causing pathogens. An exception was M. agalactiae, where danofloxacin and oxytetracycline obtained low values. However, after adding serum, gamithromycin showed the lowest MIC50 for S. aureus, Streptococcus spp., and CNS. The lowest MIC50 was obtained with all the antibiotics tested (< 0.125 µg/ml) against M. agalactiae. Supplementing with serum resulted in a significant variation in tildipirosin and gamithromycin MIC values for CNS, S. aureus, M. agalagtiae, and E. coli. In brief, the MIC for antibiotics used against mastitis should be determined under conditions closely resembling intramammary infections to obtain representative susceptibility patterns against mastitis pathogens. Caprine mastitis pathogens were broadly susceptible to danofloxacin under standard conditions. The potency of macrolides against caprine mastitis pathogens increases when serum is present in culture media.