Browsing by Subject "Flavonoides"

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    Beneficial Effects of Different Flavonoids on Vascular and Renal Function in L-NAME Hypertensive Rats
    (MDPI, 2018-04-13) Paredes, María Dolores; Romecín, Paola; Atucha, Noemí. M.; O'Valle, Francisco; Castillo, Julián; Ortiz, M. Clara; García-Estañ, Joaquín; Fisiología
    Background: we have evaluated the antihypertensive effect of several flavonoid extracts in a rat model of arterial hypertension caused by chronic administration (6 weeks) of the nitric oxide synthesis inhibitor, L-NAME. Methods: Sprague Dawley rats received L-NAME alone or L-NAME plus flavonoid-rich vegetal extracts (Lemon, Grapefruit + Bitter Orange, and Cocoa) or purified flavonoids (Apigenin and Diosmin) for 6 weeks. Results: L-NAME treatment resulted in a marked elevation of blood pressure, and treatment with Apigenin, Lemon Extract, and Grapefruit + Bitter Orange extracts significantly reduced the elevated blood pressure of these animals. Apigenin and some of these flavonoids also ameliorated nitric oxide-dependent and -independent aortic vasodilation and elevated nitrite urinary excretion. End-organ abnormalities such as cardiac infarcts, hyaline arteriopathy and fibrinoid necrosis in coronary arteries and aorta were improved by these treatments, reducing the end-organ vascular damage. Conclusions: the flavonoids included in this study, specially apigenin, may be used as functional food ingredients with potential therapeutic benefit in arterial hypertension
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    Efecto de flavonoides (extractos de fresno, spergularia y granada) sobre ratones diabéticos db/db.
    (2013-12-04) Vilaplana Vivo, Violeta; Vicente Ortega, Vicente; Gómez García, Francisco José; García Carrillo, Nuria; Facultad de Medicina
    La DM constituye un trastorno metabólico caracterizado por niveles elevados de glucosa en sangre o hiperglucemia, ocasionado por defectos en la secreción de insulina, la mala función de la misma o más frecuentemente de ambos, que suele asociarse con lesiones secundarias en diversos órganos, especialmente vasos sanguíneos, ojos, riñones, páncreas, sistema nervioso, etc. En los últimos años, las sustancias polifenólicas han recibido una gran atención debido a su potencial para la prevención de algunas enfermedades crónicas muy prevalentes, gracias a su actividad antioxidante de reducción del estrés oxidativo. El objetivo de nuestro trabajo experimental ha sido conocer los efectos de tres productos antioxidantes: extractos de fresno, spergularia y granada, en un modelo animal de DM tipo 2, a través de la evolución del peso corporal y el índice de conversión; los marcadores relacionados con la glucemia; la histomorfometría del páncreas y el deterioro cognitivo (biomarcadores y test de conducta). Para la realización del estudio, utilizamos 40 ratones LepRdb/db hembra, divididos en cuatro grupos (n=10), control y tres de tratamiento con los extractos citados. A todos los animales se les efectuaron determinaciones sanguíneas de glucosa y medida de los pesos semanalmente durante todo el experimento y a los 3, 5 y 7 meses se les realizó el estudio bioquímico. Al finalizar el estudio los animales fueron sacrificados y se tomaron muestras de páncreas para su posterior estudio histológico. Los resultados más relevantes consistieron en un menor incremento de peso e índice de conversión a partir del tercer-cuarto mes de los animales tratados. Así mismo, los animales de los grupos de tratamiento presentaron menor resistencia a la insulina, mostrando el grupo E. fresno los niveles mas bajos de glucemia y hemoglobina glicosilada. A nivel histológico este grupo también fue el que presentó mayor porcentaje de islotes pancreáticos. También el uso de los polifenoles mejoró la función cognitiva de los animales tanto a nivel bioquímico como a nivel conductual. En conclusión, los polifenoles estudiados en este modelo de DM tipo 2 mostraron una reducción del peso corporal de los animales así como el índice de conversión alimenticio; una ligera reducción en la resistencia a la insulina en todos los grupos tratados, que en el caso del grupo tratado con E. fresno se asociaba también a una disminución de los niveles de glucemia y un mayor número de islotes pancreáticos. Todos los tratamientos evidenciaron una mejora en la función cognitiva. ABSTRACT: DM is a metabolic disorder characterised by high blood sugar levels, or hyperglycemia, as a result of defects in the secretion of insulin, the malfunctioning of the same or, most frequently, both. The disorder is commonly associated with secondary lesions in several organs, including blood vessels, the eyes, kidneys, pancreas, nervous system. In recent years polyphenolic substances have received much attention for their potential to prevent several prevalent chronic diseases due to their antioxidant activity or ability to reduce oxidative stress. The aim of the experimental study described was to know the effects of three antioxidative products - ash, spergularia and pomegranate extracts – in a DM type 2 animal model, by following the body weight and conversion index, glycemia-related markers pancreatic histomorphometry and cognitive deterioration (biomarkers and behaviour test). For the study, 40 female LepRdb/db mice were divided into four groups of ten animals – a control group and one group for each of the above mentioned treatments. Blood glucose levels and body weight were measured weekly in all the animals, and a biochemical analysis was made after 3, 5 and 7 months. At the end of the experiment the animals were sacrificed and pancreas simples were taken for histological study. The most important results concerned the lower body weight increase and conversion index in the treated animals after the 3th-4th month of treatment. The same animals showed lower resistance to insulin, the group treated with ash extract showing the lowest glycemia and glycosylated haemoglobin levels. At the histological level, the same group showed the highest percentage of pancreatic islets. The use of polyphenols also improved the cognitive functions of the animals both at biochemical and behavioural levels. In conclusion, the polyphenols studied in this DM type 2 model led to a reduction in body weight and conversion index. The slight reduction in resistance to insulin in the treated groups was associated, in the case of the ash extract treatment, with a diminution of glycemia levels and greater number of pancreatic islets. All the treated groups showed an improved cognitive function.
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    Efecto de los flavonoides sobre la función endotelial en ratas con hipertensión arterial
    (2015-09-03) Paredes Carreras, María Dolores; García-Estañ López, Joaquín; Ortiz Ruiz, María Clara; Castillo Sánchez, Julián; Facultad de Medicina
    Introducción De acuerdo con las estadísticas de la Organización Mundial de la Salud (OMS), las enfermedades cardiovasculares constituyen la principal causa de muerte en los países desarrollados, siendo la hipertensión arterial (HTA) su principal factor de riesgo confirmando, por tanto, que sigue siendo un problema mundial de salud pública. Durante las últimas décadas, se han logrado avances significativos en el tratamiento de la hipertensión mediante el uso de inhibidores del sistema renina-angiotensina (SRAA), ya sea con inhibidores de la enzima convertidora de angiotensina (IECAs), antagonistas de los receptores de angiotensina II de tipo 1 (ARA II), diuréticos, betabloqueantes o bloqueantes de los canales de calcio. Un número creciente de estudios epidemiológicos observacionales han examinado la asociación entre la ingesta de alimentos ricos en polifenoles y flavonoides, con el desarrollo y evolución de ciertas enfermedades crónicas. En general, la evidencia epidemiológica muestra que una mayor ingesta de flavonoides se asocia con un menor riesgo de padecer enfermedades cardiovasculares. El creciente interés en la búsqueda de soluciones farmacológicas eficaces y carentes de efectos secundarios significativos, y su potencial papel en la regulación de la función cardiovascular, ha supuesto en los últimos años un incremento en los estudios de aplicación de estos compuestos, incidiendo en la necesidad de establecer asimismo sus vías y mecanismos de actuación, tanto molecular como fisiológicamente. Objetivos El objetivo principal de esta Tesis ha sido evaluar el efecto de distintos grupos de flavonoides en el tratamiento de la HTA experimental. Además, se han estudiado algunos de los mecanismos potencialmente responsables de este efecto, intentando, al mismo tiempo, establecer una relación estructura-actividad para los diferentes flavonoides estudiados. Metodología Se realizó un estudio metabólico de 24 horas para medir la diuresis, natriuresis y balance de sodio y obtener muestras de orina. Posteriormente, se realizaron medidas de la presión arterial media (PAM), se obtuvieron muestras de plasma y riñón, y se aisló la aorta torácica para evaluar la respuesta a acetilcolina (ACh) dependiente de endotelio. Para estimar los niveles de óxido nítrico (NO), se determinó la excreción urinaria de nitritos y, como biomarcador del estrés oxidativo, se midieron las sustancias reactivas al ácido tiobarbitúrico (TBARS) en riñón, orina y plasma. Para ello, se han utilizado dos modelos de HTA experimental: a) Hipertensión arterial por déficit de óxido nítrico o modelo de hipertensión L-NAME b) Hipertensión arterial espontánea (SHR) Resultados Los ensayos y experimentos de este trabajo, muestran que los extractos de flavonoides empleados, reducen significativamente la presión arterial media (PAM) y mejoran la mayoría de los parámetros fisiológicos estudiados, siendo sus estructuras esenciales para esta mejora. Conclusiones Resulta evidente, que existe una relación directa entre la estructura molecular de estos flavonoides y su actividad biológica específica en cada uno de los parámetros evaluados en la presente memoria. Palabras clave: Hipertensión arterial (HTA); inhibidores de la enzima convertidora de angiotensina (IECA); óxido nítrico (NO); relajación dependiente de endotelio; reactividad vascular; estrés oxidativo (EO); Nω-nitro-L-arginina metil éster (L-NAME); rata espontáneamente hipertensa (SHR); presión arterial (PA); flavonoides. Introduction According to statistics from the World Health Organization (WHO), cardiovascular diseases are the major cause of death in developed countries, the arterial hypertension (HTA) is the main risk factor confirming therefore, remains a global public health problem. During the last decades, significant progress in the treatment of hypertension has been made through the use of inhibitors of the renin-angiotensin system; with either angiotensin-converting enzyme inhibitors or angiotensin receptor type 1 blockers, diuretics, beta-blockers, and calcium channel blockers. A growing number of observational epidemiological studies have examined the association between the intake of foods rich in polyphenols and flavonoids, with the development and evolution of certain chronic diseases. Overall, the epidemiological evidence generally shows that a higher flavonoid intake is associated with lower cardiovascular disease. The increasing interest in the search for effective and safe pharmacological solutions lacking of significant side effects and potential role in the regulation of cardiovascular function, has lead in recent years to an increase in the studies of these compounds, stressing the need to further establish their pathways and mechanisms of action, both molecular and physiologically. Objectives The main objective of this thesis was to evaluate the effect of different groups of flavonoids in the treatment of experimental hypertension. In addition, we have studied some potential mechanisms responsible for this effect, trying at the same time, establish a structure-activity relationship for the different flavonoids studied. Methodology A metabolic study of 24 hours was performed to measure the diuresis, natriuresis and sodium balance and obtain urine samples. Then, we measured mean arterial pressure (MAP), heart, kidney and plasma samples were obtained by isolating the thoracic aorta to evaluate the response to acetylcholine (ACh) endothelium dependent. To estimate the levels of nitric oxide (NO), nitrite urinary excretion was determined and as a biomarker of oxidative stress, we measured the thiobarbituric acid reactive substances (TBARS) in kidney, urine and plasma. We used two experimental models of experimental hypertension: a) N (G)-nitro-L-arginine methyl ester (L-NAME) induced hypertension rats b) Spontaneously Hypertensive Rats (SHR) Results Tests and experiments of this work show that the flavonoid extracts used, significantly reduce mean arterial pressure (PAM) and improve most physiological parameters studied being their structures critical for this improvement. Conclusions It seems evident, as main conclusion, that there is a direct relationship between the molecular structure of these flavonoids and their specific biological activity in each of the parameters evaluated herein. Key words: Arterial hypertension (HTA); inhibitors of the renin-angiotensin system (IECA); nitric oxide (NO); endothelium-dependent relaxation; vascular reactivity; oxidative stress; Nω-nitro-L-arginine methyl ester (L-NAME); spontaneously hypertensive rats (SHR); blood pressure (BP); flavonoids.
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    Efectos saludables de flavonoides. Estudio experimental in vitro e in vivo
    (Universidad de Murcia, 2010-07-20) Álvarez Sánchez, Nuria; Vicente Ortega, Vicente; Alcaraz Baños, Miguel; Departamentos y Servicios::Departamentos de la UMU::Dermatología, Estomatología, Radiología y Medicina Física
    La apigenina (4’, 5, 7 trihidroxiflavona), flavonoide presente en distintos vegetales, tiene numerosas características saludables por las cuales elegimos un derivado hidrosoluble, la apigenina potásica, para el presente estudio. Hemos estudiado su actividad frente a la inflamación aguda, al cáncer de próstata y a los daños causados por las radiaciones, tanto ionizantes como no ionizantes (radiación UV), utilizando diversas técnicas, tanto in vitro como en modelos in vivo. Este flavonoide demostró actividad antiinflamatoria, reduciendo la inflamación hasta un 78%. Asimismo, la apigenina potásica mostró actividad quimiopreventiva frente al cáncer de próstata, al reducir la viabilidad y la migración e inducir apoptosis in vitro, y aumentar la supervivencia de animales con tumores. Además, la apigenina potásica presentó efecto geno- y citoprotector frente a la radiación ionizante (radiación ? y X), con un factor de protección de un 27-35 % en linfocitos humanos, y de un 50-86 % en dos líneas celulares de próstata. Por último, el flavonoide ha demostrado proteger frente al fotoenvejecimiento causado por la radiación UV, reduciendo la displasia epitelial y la elastosis dérmica, dos marcadores de cáncer cutáneo; además, ha sido posible detectar la apigenina en diversos tejidos, entre ellos el cerebro. Abstract: Apigenin (4’, 5, 7 trihidroxyflavone), a flavonoid present in different plants, shows some healthy characteristics for which a water soluble derivated, potassium apigenin, was chosen for this study. It has been studied the activity of potassium apigenin against acute inflammation, prostate cancer and the effect of ionising and non-ionising (UV) radiations, using different techniques, both in vitro and in vivo models. This flavonoid showed anti-inflammatory effects, inhibiting the inflammation by up to 78%. It also demonstrated chemopreventive activity against prostate cancer, by reducing the cell viability and migration, inducing apoptosis and increasing the animal survival. Moreover, potassium apigenin showed genoprotector and citoprotector effects against ionising radiation (radiation ? and X), with a protection factor of 27-35% in human lymphocytes and of 50-86% in two prostate cell lines. Finally, the flavonoid protected from the photoaging induced by UV radiation, diminishing epithelial dysplasia and dermal elastosis, two markers of skin cancer; furthermore, potassium apigenin was detected in some tissues, brain among them.
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    Effects of flavonoids in experimental models of arterial hypertension
    (Bentham Science Publishers, 2021-08-12) Marín Atucha, Noemí; Romecín, Paola; Vargas, Félix; García-Estañ López, Joaquín; Fisiología; Facultades de la UMU::Facultad de Medicina
    Flavonoids are a class of substances of a vegetal origin with many interesting actions from the point of view of human disease. Interest in flavonoids in the diet has increased in recent years due to the publication of basic, clinical and epidemiological studies that have shown a whole array of salutary effects related to intake of flavonols and flavones as well as a lower morbility and mortality of cardiovascular diseases. Since arterial hypertension is the most common modifiable risk factor for cardiovascular diseases, this review will focus mainly on the effects of flavonoids on the cardiovascular system with relation to the elevation of blood pressure. Its antihypertensive effects as well as the many investigations performed in experimental models of arterial hypertension, are reviewed in this mini-review.
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    Flavonoids in Kidney Health and Disease
    (Frontiers, 2018-04-24) Vargas, Félix; Romecín, Paola; Wangesteen, Rosemary; Atucha, Noemí M.; García-Estañ, Joaquín; García-Guillén, Ana I.; Vargas-Tendero, Pablo; Paredes, M. Dolores; Fisiología
    This review summarizes the latest advances in knowledge on the effects of flavonoids on renal function in health and disease. Flavonoids have antihypertensive, antidiabetic, and antiinflammatory effects, among other therapeutic activities. Many of them also exert renoprotective actions that may be of interest in diseases such as glomerulonephritis, diabetic nephropathy, and chemically-induced kidney insufficiency. They affect several renal factors that promote diuresis and natriuresis, which may contribute to their well-known antihypertensive effect. Flavonoids prevent or attenuate the renal injury associated with arterial hypertension, both by decreasing blood pressure and by acting directly on the renal parenchyma. These outcomes derive from their interference with multiple signaling pathways known to produce renal injury and are independent of their blood pressure-lowering effects. Oral administration of flavonoids prevents or ameliorates adverse effects on the kidney of elevated fructose consumption, high fat diet, and types I and 2 diabetes. These compounds attenuate the hyperglycemia-disrupted renal endothelial barrier function, urinary microalbumin excretion, and glomerular hyperfiltration that results from a reduction of podocyte injury, a determinant factor for albuminuria in diabetic nephropathy. Several flavonoids have shown renal protective effects against many nephrotoxic agents that frequently cause acute kidney injury (AKI) or chronic kidney disease (CKD), such as LPS, gentamycin, alcohol, nicotine, lead or cadmium. Flavonoids also improve cisplatin- or methotrexate-induced renal damage, demonstrating important actions in chemotherapy, anticancer and renoprotective effects. A beneficial prophylactic effect of flavonoids has been also observed against AKI induced by surgical procedures such as ischemia/reperfusion (I/R) or cardiopulmonary bypass. In several murine models of CKD, impaired kidney function was significantly improved by the administration of flavonoids from different sources, alone or in combination with stem cells. In humans, cocoa flavanols were found to have vasculoprotective effects in patients on hemodialysis. Moreover, flavonoids develop antitumor activity against renal carcinoma cells with no toxic effects on normal cells, suggesting a potential therapeutic role in patients with renal carcinoma.
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    Inhibición de la función de las plaquetas por flavonoides : implicación de la ruta de señalización del tromboxano A2 / José Antonio Guerrero López; [directores, José Rivera Pozo y María Luisa Lozano Almela].
    (Murcia : Universidad de Murcia, Departamento de Bioquímica y Biología Molecular B e Inmunología,, 2005) Guerrero López, José Antonio
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    Initial growth and production of secondary metabolites in Basella alba leaves cultivated in a sandy textured soil with addition of organic and organo-mineral substrate
    (Universidad de Murcia. Servicio de publicaciones, 2022) Pina, José Carlos; Morbeck-de-Oliveira, Ademir Kleber; Matias, Rosemary
    Crecimiento inicial y producción de metabolitos secundarios en hojas de Basella alba cultivadas en un suelo de textura arenosa con adición de sustrato orgánico y organomineral La especie Basella alba se conoce popularmente como bertalha india y se consume ampliamente en forma de ensaladas, por ejemplo. El objetivo de este estudio fue evaluar las etapas iniciales de crecimiento y la producción de metabolitos secundarios en diferentes sustratos. Transcurridos 90 días desde el inicio de la plantación (pleno sol), en suelo arenoso mezclado con diferentes sustratos, se determinó el efecto de los sustratos sobre el crecimiento y producción de metabolitos secundarios en las hojas. Los resultados indicaron que el tratamiento con la adición de 70% de vermicompost indujo el mayor crecimiento y la mejor concentración de compuestos fenólicos y flavonoides. Sin embargo, la mayoría de los tratamientos dieron como resultado la producción de heterósidos cardiotónicos, que pueden causar problemas de salud.
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    Moderate Effect of Flavonoids on Vascular and Renal Function in Spontaneously Hypertensive Rats
    (MDPI, 2018-08-16) Paredes, María Dolores; Romecín, Paola; Atucha, Noemí M.; O'Valle, Francisco; Castillo, Julián; Ortiz, M. Clara; García-Estañ, Joaquín; Fisiología
    Many studies have shown that flavonoids are effective as antihypertensive drugs in arterial hypertension. In the present work, we have analyzed the effects of some flavonoid extracts in the spontaneous hypertensive rat model (SHR). An important feature of this study is that we have used a low dose, far from those that are usually applied in human therapy or experimental animals, a dose that responded to the criterion of a potential future commercial use in human subjects. Treatments were carried out for 6 and 12 weeks in two groups of SHR rats, which received apigenin, lemon extract, grapefruit + bitter orange (GBO) extracts, and cocoa extract. Captopril was used as a positive control in the SHR group treated for 6 weeks (SHR6) and Diosmin was used as the industry reference in the SHR group treated for 12 weeks (SHR12). Captopril and GBO extracts lowered the high arterial pressure of the SHR6 animals, but none of the extracts were effective in the SHR12 group. Apigenin, lemon extract (LE), GBO, and captopril also improved aortic vascular relaxation and increased plasma and urinary excretion of nitrites, but only in the SHR6 group. Kidney and urinary thiobarbituric acid reactive substances (TBARS) were also significantly reduced by GBO in the SHR6 rats. Apigenin also improved vascular relaxation in the SHR12 group and all the flavonoids studied reduced urinary thiobarbituric acid reactive substances (TBARS) excretion and proteinuria. Vascular abnormalities, such as lumen/wall ratio in heart arteries and thoracic aorta, were moderately improved by these treatments in the SHR6 group. In conclusion, the flavonoid-rich extracts included in this study, especially apigenin, LE and GBO improved vascular vasodilatory function of young adult SHRs but only the GBO-treated rats benefited from a reduction in blood pressure. These extracts may be used as functional food ingredients with a moderate therapeutic benefit, especially in the early phases of arterial hypertension
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    Naringina y sus efectos a nivel densitométrico sobre la regeneración guiada con extracto de uva y colágeno de origen porcino : estudio en modelo experimental con conejos albinos de Nueva Zelanda
    (Universidad de Murcia, 2018-02-02) López Lozano, Sergio; Gómez García, Francisco José; García Carrillo, Nuria; Facultades, Departamentos, Servicios y Escuelas::Facultades de la UMU::Facultad de Medicina
    El propósito principal de este estudio experimental ha consistido en calibrar y comprender, desde el punto de vista densitométrico a nivel óseo, la efectividad de los biomateriales que aplicamos en nuestros animales, en este caso, conejos, con el fin de valorar su extrapolación a seres humanos en futuros ensayos clínicos en el ámbito de la implantología. Se depositaron los biomateriales (naringina sola, naringina con colágeno y naringina con extracto de uva) en lechos de las tibias, maxilares y mandíbulas de 7 conejos albinos de Nueva Zelanda. Se realizó, tras su sacrificio y obtención de los resultados en unidades Hounsfield a través del micro-TC, un análisis densitométrico, 6 meses después de la implantación del biomaterial. Objetivos. -Estudiar los cambios densitométricos que se producen durante la curación de defectos óseos creados en tibia, maxilar y mandíbula de conejos albinos de Nueva Zelanda, mediante densitometría. -Estudiar el efecto del relleno de dichos defectos con naringina, sola y en combinación con extracto de uva y/o colágeno. -Determinar la correlación anatómica en términos de densidad, en tibia, maxilar y mandíbula, mediante el estudio con micro-tomografía computarizada (micro-TC). Resultados. El porcentaje global de score positivos (1+ y 2+), donde se integran todos los grupos que presentan naringina sola o bien combinada con colágeno o extracto de uva, resultó ser de un 73%, si bien este porcentaje varía según el ámbito de aplicación o en función de la combinación, si la hubiera. Así, la naringina sola, reportó un porcentaje de 71% de score positivos frente a un 29% de las muestras que no indicaron cambios que indiquen una mejoría densitométrica o score (0). La naringina en combinación con colágeno reportó un 85% de score positivos (1+ y 2+) frente a un 15% de score (0). La amalgama resultante de naringina en combinación con extracto de uva arrojó un resultado de 62% de score positivos frente a un 38% de score (0). Conclusiones. La microtomografía computerizada es una técnica adecuada para evaluar los cambios densitométricos tras la realización de defectos óseos quirúrgicos, existiendo correlación entre los aspectos morfológicos y los datos densitométricos.En nuestro modelo, el tratamiento con naringina favoreció la neoformación ósea en los defectos, siendo la combinación más efectiva, la formada por naringina y colágeno.Probablemente, no hayamos observado mayores diferencias entre los distintos grupos experimentales debido a que el periodo de estudio fue relativamente largo. Naringin and its densitometric effects on guided regeneration with grape extract and porcine collagen. Study in experimental model with albino rabbits from New Zealand. The main purpose of this experimental study was to calibrate and understand, from the densitometric point of view at the bone level, the effectiveness of the biomaterials we apply to our animals, in this case, rabbits, in order to assess their extrapolation to the human beings at future clinical trials in the field of implantology. Biomaterials (naringin alone, naringin with collagen and naringin with grape extract) were deposited in beds of the tibia, maxillary and jawbone of 7 New Zealand albino rabbits. Analysis was performed after slaughter, and the results were obtained in Hounsfield units through the microTC, a densitometric analysis, 6 months after implantation of the biomaterial. Objectives. -To study the densitometric changes that occur during the healing of bone defects created in tibia, maxillary and jawbone of New Zealand albino rabbits by densitometry. -To study the effect of filling these defects with naringin, alone and in combination with grape extract and / or collagen. -To determine the anatomical correlation in terms of density, in tibia, maxillary and jawbone, by the study with computerized microtomography (microTC). Results. The overall percentage of positive scores (1+ and 2+), where all the groups that present naringin alone or combined with collagen or grape extract, were found to be 73%, although this percentage varies according to the scope of application or depending on the combination, if any. Thus, naringin alone, reported a percentage of 71% of positive score compared to 29% of the samples that did not indicate changes that indicated a densitometric improvement or score (0). Naringin in combination with collagen reported a positive 85% score (1+ and 2+) versus a 15% score (0). The resulting amalgam of naringin in combination with grape extract yielded a 62% positive score versus a 38% score (0). Conclusions. The computerized microtomography is a suitable technique to evaluate the densitometric changes after the accomplishment of surgical bone defects, being there correlation between the morphological aspects and the densitometric data. In our model, the treatment with naringin favored the new bone formation in the defects, being the combination more effective, formed by naringin and collagen. Probably, we have not observed greater differences between the different experimental groups because the study period was relatively long.