Browsing by Subject "Epoxy resin"

Now showing 1 - 2 of 2
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    Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 1998) Brorson, S. H.
    Considering the importance of immunolocalization of cellular substances combined with good ultrastructure and ease of use , this review is focused on the use of resin and the possibilities of manipulating the resin before and after embedding in order to improve the immunolabeling of resin sections for electron microscopy. The qualities of acrylic re sin s and conventional epoxy resin for immunoelectron microscopy are discussed. Acrylic sections are usually more suited for immunoelectron microscopy than conventional epoxy sections. Different etching procedures (sodium ethoxide or sodium metaperiodate) may be applied to conventional epoxy sections to enhance the yield of immunolabeling. Lately, a method which does not involve any kind of etching has been developed for enhancing the immunogold labeling of epoxy sections up to about 8 times. This method involves increased concentration of accelerator in the epoxy resin mixture when processing the tissue. The ultrastructural preservation of the tissue is important in immunoelectron microscopical procedures. and not only the intensity of the immunolabeling: in this respect no resin may compete with the widely used epoxy resins.
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    Heat-induced antigen retrieval of epoxy sections for electron microscopy
    (Murcia : F. Hernández, 2001) Brorson, S.H.
    The purpose of this manuscript is to review the literature on the use of heat-induced antigen retrieval methods to enhance the immunolabeling of epoxy sections at the electron microscopical level. The history of the development of antigen retrieval by heating formaldehyde fixed paraffin sections in a buffer solution is given in short, and how this technique has been extended to resin sections and in particular epoxy sections is explained. Theories for the mechanism of enhancement of the immunolabeling of epoxy sections by the heat-retrieval method are discussed, and it is finally speculated whether most of the mechanisms for antigen retrieval on epoxy sections in heated buffer solution are essentially the same as for conventional immunoenhancing by deplastizing and etching. The more accelerator used in the processing of the tissue the more intense the immunolabeling of the heated epoxy sections becomes. The intensity of immunolabeling of the epoxy sections increases with the temperature in the heated buffer solution, and the intensity is significantly higher at high autoclave temperatures than at 95 OC, Heat-induced antigen retrieval is also compared with other, conventional techniques for enhancing the immunolabeling of epoxy sections.