Browsing by Subject "Enzyme biocatalysis"

Now showing 1 - 2 of 2
  • Repository logo
    Publication
    Open Access
    Application of a diffusion-reaction kinetic model for the removal of 4-chlorophenol in continuous tank reactors
    (Taylor and Francis Group, 2014-03-03) Murcia Almagro, María Dolores; Gómez Gómez, María; Bastida Rodríguez, Josefa; Hidalgo Montesinos, Asunción María; Montiel Morte, María Claudia; Ortega Requena, Salvadora; Ingeniería Química
    A continuous tank reactor was used to remove 4-chlorophenol from aqueous solutions, using immobilized soybean peroxidase and hydrogen peroxide. The influence of operational variables (enzyme and substrate concentrations and spatial time) on the removal efficiency was studied. By using the kinetic law and the intrinsic kinetic parameters obtained in a previous work with a discontinuous tank reactor, the mass-balance differential equations of the transient state reactor model were solved and the theoretical conversion values were calculated. Several experimental series were used to obtain the values of the remaining model parameters by numerical calculation and using an error minimization algorithm. The model was checked by comparing the results obtained in some experiments (not used for the determination of the parameters) and the theoretical ones. The good concordance between the experimental and calculated conversion values confirmed that the design model can be used to predict the transient behaviour of the reactor.
  • Thumbnail Image
    Publication
    Open Access
    ENZYMATIC BIOSYNTHESIS OF RICINOLEIC ACID ESTOLIDES
    (Elsevier, 2005-05-31) Bódalo-Santoyo, A.; Bastida Rodríguez, Josefa; Máximo Martín, María Fuensanta; Montiel Morte, María Claudia; Murcia Almagro, María Dolores; Ingeniería Química
    Candida rugosa lipase has been shown to have sufficient activity to catalyse the enzymatic synthesis of ricinoleic acid estolides in a batch reactor. The water requirements of the reactor change during the reaction: at the beginning of the process a minimum amount of water is necessary but, later, the reaction mixture must be dried out to obtain an estolide with a high degree of condensation. The influence on the reaction rate of variables, such as water content, enzyme concentration and mixing devices, was established and optimised. Using an initial water content of 144000 ppm and a lipase concentration of 13.33 mgE/g ricin, and maintaining the temperature at 40°C by mean of hot air circulation and using a three-bladed propeller stirrer as mixing device, an estolide of ricinoleic acid with an acid number of 65 was obtained in 48 hours.