Browsing by Subject "Endodontics"

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    Bioceramic versus traditional biomaterials for endodontic sealers according to the ideal properties
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Ortega, Miguel A.; Rios, Laura; Fraile Martínez, Oscar; Liviu Boaru, Diego; Leon Oliva, Diego De; Barrena Blázquez, Silvestra; Pereda Cerquella, Claude; Garrido Gil, Maria J.; Manteca, Luis; Buján, Julia; García Honduvilla, Natalio; Garcia Montero, Cielo; Rios Parra, Antonio
    Odontology, as a scientific discipline, continuously collaborates with biomaterials engineering to enhance treatment characteristics and patients' satisfaction. Endodontics, a specialized field of dentistry, focuses on the study, diagnosis, prevention, and treatment of dental disorders affecting the dental pulp, root, and surrounding tissues. A critical aspect of endodontic treatment involves the careful selection of an appropriate endodontic sealer for clinical use, as it significantly influences treatment outcomes. Traditional sealers, such as zinc oxide-eugenol, fatty acid, salicylate, epoxy resin, silicone, and methacrylate resin systems, have been extensively used for decades. However, advancements in endodontics have given rise to bioceramic-based sealers, offering improved properties and addressing new challenges in endodontic therapy. In this review, a classification of these materials and their ideal properties are presented to provide evidence-based guidance to clinicians. Physicochemical properties, including sealing ability, stability over time and space, as well as biological properties such as biocompatibility and antibacterial characteristics, along with costeffectiveness, are essential factors influencing clinicians' decisions based on individual patient evaluations.
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    Open Access
    Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs
    (Springer, 2023-05-01) López Garcia, Sergio; Rodríguez Lozano, Francisco Javier; Sanz, José Luis; Forner, Leopoldo; Pecci Lloret, María Pilar; Lozano, Adrián; Murcia, Laura; Sánchez Bautista, Sonia; Oñate Sánchez, Ricardo Elías; Dermatología, Estomatología, Radiología y Medicina Física
    Objectives To assess the cytocompatibility and bioactive potential of the new calcium silicate-based cement Ceraputty on human periodontal ligament stem cells (hPDLSCs) compared to Biodentine and Endosequence BC root repair material (ERRM). Materials and methods hPDLSCs were isolated from extracted third molars from healthy donors. Standardized sample discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, osteo/cemento/odontogenic marker expression via RT-qPCR, and cell calcified nodule formation via Alizarin Red S staining. hPDLSCs cultured in unconditioned or osteogenic media were used as negative and positive control groups, respectively. Statistical analysis was performed using one-way ANOVA or two-way ANOVA and Tukey’s post hoc test. Statistical significance was established at p < 0.05. Results The highest Ca2+peak was detected from Biodentine samples, followed by ERRM and Ceraputty. hPDLSC viability was significantly reduced in Ceraputty samples (p < 0.001), while 1:2 and 1:4 Biodentine and ERRM samples similar results to that of the negative control (p > 0.05). Biodentine and ERRM exhibited an upregulation of at least one cemento/odonto/osteogenic marker compared to the negative and positive control groups. Cells cultured with Biodentine produced a significantly higher calcified nodule formation than ERRM and Ceraputty (p < 0.001), which were also higher than the control groups (p < 0.001). Conclusion Ceraputty evidenced a reduced cytocompatibility towards hPDLSCs on its lowest dilutions compared to the other tested cements and the control group. Biodentine and ERRM promoted a significantly higher mineralization and osteo/cementogenic marker expression on hPDLSCs compared with Ceraputty. Further studies are necessary to verify the biological properties of this new material and its adequacy as a retrograde filling material.