Browsing by Subject "Encapsulation"

Now showing 1 - 4 of 4
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    Data for compounds included in the article: "Exploring the multifaceted effects of dietary encapsulated piscidin 1 on gilthead seabream (Sparus aurata): from immunomodulation to muscle quality"
    (2026-05-22) Claudia Marín Parra; Marín Parra, Claudia; Espinosa Ruiz, Cristóbal; Coxon, Christopher R.; Esteban Abad, María de los Ángeles; María Ángeles Esteban Abad; Biología Celular e Histología; María Ángeles Esteban Abad
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    Molecular Insight into Silk Fibroin Based Delivery Vehicle for Amphiphilic Drugs: Synthesis, Characterization and Molecular Dynamics Studies
    (Elsevier B.V., 2019) Montalbán, Mercedes G.; Chakraborty, Sandipan; Peña-García, Jorge; Verli, Hugo; Víllora Cano, Gloria; Pérez-Sánchez, Horacio; Díaz Baños, F. Guillermo; Química Física
    Recent emergence of natural biopolymers as drug delivery vehicles is attributed to their biodegradability and less systemic toxicity. Here, we have synthesized curcumin, indomethacin and emodin-loaded silk fibroin nanoparticles (SFNs) and characterized several pharmacokinetic parameters (Drug Loading and Encapsulation Efficiency). Silk fibroin is a highly promising bio-material with impressive mechanical properties, high bio-compatibility and it does not exert any immunological responses in vivo. Our results show that emodin almost released completely within 144 hr, however a steady release profile has been observed for indomethacin which is attributed to its moderate loading and encapsulation efficiency by SFNs. On the other hand, complete release of curcumin is not observed even in 168 hr. Curcumin also shows very promising drug loading and encapsulation efficiency when loaded within the SFNs matrix. Molecular level characterization with the aid of blind docking and molecular dynamics simulation reveals that the encapsulation efficiency of the drugs exactly follows the interaction energy patterns obtained from MM/PBSA calculation, i.e., curcumin > indomethacin > emodin. Strong binding energy of curcumin with the fibroin protein is attributed to the formation of more number of hydrogen bonds compared to the other two drugs and involvement in additional π-π stacking interactions. Indomethacin interacts moderately with the SFN primarily mediated through several van der Waals interactions which accounts for its sustained release from the SFN matrix. Emodin interacts with the fibroin protein very weakly which is responsible for its low encapsulation and observed diffusion controlled release behavior within the fibroin matrix.
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    Stilbenes: Characterization, bioactivity, encapsulation and structural modifications. A review of their current limitations and promising approaches
    (Taylor and Francis Group, 2022-03-02) Navarro Orcajada, Silvia; Vidal Sánchez, Francisco José; Albadelejo Maricó, Lorena; Matencio Durán, Adrián; Conesa Valverde, Irene; López Nicolás, José Manuel; Vidal Sánchez, Francisco José; García Carmona, Francisco; Bioquímica y Biología Molecular A
    Stilbenes are phenolic compounds naturally synthesized as secondary metabolites by the shikimate pathway in plants. Research on them has increased in recent years due to their therapeutic potential as antioxidant, antimicrobial, anti-inflammatory, anticancer, cardioprotective and anti-obesity agents. Amongst them, resveratrol has attracted the most attention, although there are other natural and synthesized stilbenes with enhanced properties. However, stilbenes have some physicochemical and pharmacokinetic problems that need to be overcome before considering their applications. Human clinical evidence of their bioactivity is still controversial due to this fact and hence, exhaustive basis science on stilbenes is needed before applied science. This review gathers the main physicochemical and biological properties of natural stilbenes, establishes structure-activity relationships among them, emphasizing the current problems that limit their applications and presenting some promising approaches to overcome these issues: the encapsulation in different agents and the structural modification to obtain novel stilbenes with better features. The bioactivity of stilbenes should move from promising to evident.
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    The use of cyclodextrins as solubility enhancers in the ORAC method may cause interference in the measurement of antioxidant activity
    (Elsevier, 2022-02-26) Navarro Orcajada, Silvia; Rodríguez Bonilla, Pilar; Matencio Durán, Adrián; Conesa Valverde, Irene; López Nicolás, José Manuel; García Carmona, Francisco; Bioquímica y Biología Molecular A
    The ability of cyclodextrins to enhance the water solubility of lipophilic compounds is used to modify the waterbased Oxygen Radical Absorbance Capacity (ORAC) method to measure antioxidant activity in vitro. However, the use of these solubility enhancers may alter fluorescent readings, which has led to contradictory results being described in the literature. The low specificity of these oligosaccharides and their controlled release effect can result in cyclodextrins forming inclusion complexes with other reagents in the assay, changing the kinetics. In this study, the cause of cyclodextrins’ interference in the ORAC method is evaluated through a physicochemical and computational approach. Cyclodextrins showed a clear increase in the fluorescent signal both in the presence and absence of the antioxidant oxyresveratrol, the precise effect being dependent on the type and concentration of cyclodextrin. Although the glucidic nature of cyclodextrins could play a minimal role in this effect, it seems that the main cause was the encapsulation of other substrates in the reaction, fluorescein and AAPH.