Browsing by Subject "Elastin"

Now showing 1 - 4 of 4
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    Abnormal elastin and collagen deposition is present in extracranial arteriovenous malformations: A comparison to intracranial disease
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2019) Wei, Ting; Shalin, Sara; Draper, Elizabeth; Miller, Emily; Zhang, Haihong; Sun, Ravi; Lee, Madison; Albert, Gregory; Richter, Gresham T.
    Background. Vascular malformations are characterized by anomalous vascular channels with fragile walls and a propensity to bleed. Arteriovenous malformations (AVMs) in particular have disorganized vascular spaces with intervening fibrosis. Characterization of the structural abnormalities of these vessels has not been comprehensively evaluated. We hypothesize that AVMs are likely to demonstrate altered elastic and collagen fiber organization and distribution, reflecting their fragility, vascular instability, and abnormal development. Methods. Fifteen AVMs were histologically evaluated by H&E, elastin and trichrome staining. To identify potential differences between extracranial and intracranial AVMs, 5 AVMs were harvested from the brain (n=5) and 10 from extracranial sites involving the skin and deep soft tissue (n=10). Results. The elastin staining demonstrated reduplication, fragmentation and disruption of internal elastic lamina as well as irregular thickness, and inconsistent vascular density of all AVM specimens. Trichrome staining revealed thickening of the intimal layers of AVM arteries and demonstrated an irregular thickness of venous walls within the malformation and some areas of medial degeneration. Intracranial AVMs are characterized by more intramural inflammation with predominant neutrophil and lymphocyte infiltration. In contrast, extracranial AVMs display more extravascular inflammation with mast cell and neutrophil infiltration. Microvascular proliferations intervening between larger blood vessels were also noted in both types of AVMs, but more obvious in extracranial AVMs. Conclusion. These observed histologic anomalies of AVMs demonstrate disorganized deposition of elastin and collagen that point to the clinically observed vascular instability and fragility of these lesions
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    Elastin variations implicating in vascular smooth muscle cells phenotype in human tortuous arteries
    (Murcia : F. Hernández, 2000) Ortiz, P.P.; Sarrat, R.; Daret, D.; Whyte, J.; Torres, A.; Daniel-Lamaziere, J.M.
    The aim of the present work was to study the morphological implications between the elastin and the phenotypic expression of the vascular smooth muscle cells. For this purpose, sixty human tortuous arteries from different territories have been studied. We have measured the morphometric indexes Intimal Thickening Index and Elastolyse Index and they have been quantified with computer system analysis, image-colour corresponding to the orcein and Verhoeff reactions for detecting elastin and the a-actin in the smooth muscle cells. We compared both territorial arteries from the cranial and from abdominal origin. The elastin concentration was similar in both territories, but not its morphology according to its spatial distribution. We have observed a relationship between the elastin structural organisation from the media of arteries and of the internal elastic lamina in these territories and the variation of reactivity to the smooth muscle a-actin as a marker of the phenotypic state. Our results confirm the hypothesis that elastin, besides intervening in the architecture of the arterial wall, is a factor implicated in the phenotypic variability of the smooth muscle cells and in the development and evolution of the intimal thickenings in human atherosclerosis.
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    Extracellular matrix molecules associated with lymphatic vessels in health and disease
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2024) Czarnowska, Elżbieta; Ratajska, Anna; Jankowska Steifer, Ewa; Flaht Zabost, Aleksandra; Niderla Bielińska, Justyna
    Lymphatic vessels (LyVs), responsible for fluid, solute, and immune cell homeostasis in the body, are closely associated with the adjacent extracellular matrix (ECM) molecules whose structural and functional impact on LyVs is currently more appreciated, albeit not entirely elucidated. These molecules, serving as a platform for various connective tissue cell activities and affecting LyV biology should be considered also as an integral part of the lymphatic system. Any alterations and changes in ECM molecules over the course of disease impair the function and structure of the LyV network. Remodeling of LyV cells, which are components of lymphatic vessel walls, also triggers alterations in ECM molecules and interstitial tissue composition. Therefore, in this review we aimed to present the current knowledge on ECM in tissues and particularly on molecules surrounding lymphatics in normal conditions and in disease
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    The role of extracellular and intracellular proteolytic systems in aneurysms of the ascending aorta
    (Universidad de Murcia. Departamento de Biología Celular e Histología, 2016) Werner, Isabella; Schack, Stephanie; Richter, Manfred; Stock, Ulrich A.; El-Sayed Ahmad, Ali; Moritz, Anton; Beiras-Fernandez, Andres