DigitalUM :: Browsing by Subject "Diazo-reaction"

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Diazo-reaction positive substance observed in the cortex of Chattonella antiqua
(Murcia : F. Hernández, 1986) Masahisa Shimada; Ryoichi Shimono; Tomio Imahayashi; Hiroki H. Ozaki; Tetuhide H. Murakami
In order to investigate the causative factors responsible for removal of mucous coat from the gill lamellae of young yellowlail, Seriola quinqueradiata by red tide, diazo-reactions were employed for planktons and their media. The concentration of NO,- in the medium containing the raphidophyceae, Chatonella antiqua (ca 2000 cells/ml), was 0.70+0.05 (p g/mlk SEM). In addition, diazo-reaction positive substances (NO,) which may degenerate the mucous, was highly concentrated in the cortex (perikaryon) of Chattonella antiqua. Morphologically, mucocysts, and chloroplats were likewise present in the cortex. Mucocysts were packed with fine fibrous content. Histochemically, the mucocysts were stained with PAS and had an abundance of nitrogen oxides (NO,). We observed discharge of the fibrous material from the mucocysts. These results suggest that when Chattonella antiqua is passing between the gill lamellae, N0,discharged from the mucocysts may act on the mucous, leading to the degeneration and concomitant removal of the mucous coat from gill lamellae.