Browsing by Subject "Citoquininas"

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    Influence of cytokinins and subculturing on proliferation capacity of single-axillary-bud microcuttings of Vitis vinifera L. cv. Napoleón
    (Murcia: Universidad de Murcia, Servicio de Publicaciones, 2003) Ibáñez-Torres, Ascensión; Valero, Manuel; Morte Gómez, María Asunción; Biología Vegetal; Facultad de Biología
    The addition of cytokinins to the culture medium was essential for the sprouting and formation of multiple shoots from axillary-bud microcuttings excised from in vitro-grown plants of Vitis vinifera L. cv. Napoleón. Of three cytokinins assayed, 6-benzyladenine (BA), kinetin (K), 2 isopentenyladenine (2iP), and thidiazuron (TDZ), BA encouraged the best morphogenic response. This is especially true at concentrations of 6.67 and 8.9 µM which led to 100% sprouting, an average of 8.7 axillary buds and 2.5 shoots developed per explant, together with maximum multiplication coefficients of buds and shoots. Subculture of microcuttings to fresh media maintained sprouting rates and substantially increased the production of shoots and axillary buds per explant until the third subculture. According to the results obtained it could seem advisable to limit the multiplication cycles for cv. Napoleón to three since further transfers induced vitrification phenomema and degenerations.