Browsing by Subject "Cerebrospinal fluid"

Now showing 1 - 4 of 4
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    Alterations of the cerebrospinal fluid proteins and subcommissural organ secretion in the arterial hypertension and ventricular dilatation. A study in SHR rats
    (Murcia : F. Hernández, 2006) Martínez de la Peña y Valenzuela, Isabel; Carmona-Calero, E.; Pérez-González, H.; Ormazabal-Ramos, C.; Fernández-Rodríguez, P.; Gonzalez-Marrero, I.; Castañeyra Perdomo, A.; Ferres Torres, R.
    The aim of this work was to analyze the proteins in the cerebrospinal fluid (CSF) of spontaneously hypertensive rats, to study their possible role in the relationship between hydrocephalus, arterial hypertension and alterations in the subcommissural organ. Brains from control Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) sacrificed with chloral hydrate were used. Antiserums against some cerebrospinal fluid protein bands and Reissner’s fiber (RF) were used for immunohistochemical study of the SCO. Ventricular dilation was observed in the lateral and third ventricle of the SHR. Third ventricle ependyma showed immunoreactive material (IRM) for antibody against 141kDa protein band anti-B1 and 117 protein band anti-B2 and the SCO of the SHR showed a decrease of the IRM when compared with WKY rats. An alteration in the expression of anti-RF was found to compare the SCO of the WKY and SHR groups. Our results demonstrate that hydrocephalus and hypertension are interconnected in this kind of rat which produce alterations in SCO secretions and some proteins of the CSF.
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    Canine C-reactive protein measurements in cerebrospinal fluid by a time-resolved immunofluorimetric assay
    (SAGE Publications, 2011-01-01) Martínez Subiela, Silvia; Caldin, Marco; Parra Muñoz, María Dolores; Ottolini, Nicola; Bertolini, Giovanna; Bernal, Luis J.; García Martínez, Juan Diego; Cerón Madrigal, José Joaquín; Ciencias Sociosanitarias
    In the current study, the quantification of C-reactive protein (CRP) in cerebrospinal fluid (CSF) of dogs using an adapted time-resolved immunofluorimetric assay (TR-IFMA) was investigated, as well as whether the assay could be used to detect the range of CRP concentrations found in different clinical situations. Intra- and interassay coefficients of variation were below 15% in all cases. The TR-IFMA measured the CRP values in a proportional and linear manner (r 5 0.99); also CRP concentrations measured in CSF and in serum were significantly correlated (r 5 0.80, P 5 0.003). The limit of detection of the method was 7.1 3 1026 mg/l. The assay was able to detect differences in CRP concentrations in CSF of dogs with inflammatory disorders compared with dogs with spinal cord compression or idiopathic epilepsy. In conclusion, TR-IFMA constitutes a very sensitive, precise, and accurate method for the measurement of CRP concentrations in CSF.
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    La disfunción del sistema de derivación ventriculoperitoneal: implicación de enfermería de urgencias.
    (Murcia : Servicio de Publicaciones de la Universidad de Murcia, 2009) Luque Oliveros, M.; Bullon, M.; Peña, A.
    La función de los profesionales facultativos de enfermería en un Servicio de Cuidados Críticos y Urgencias es fundamental. Prioritario para el encuadre y rapidez de la resolución de los pacientes que acuden con distintas patologías. No obstante, es muy desconocida tanto por el resto del personal sanitario, como de los usuarios que acuden a un hospital. En nuestro caso la demora del tratamiento de una hidrocefalia puede desembocar en el enclavamiento cerebral y por consiguiente la muerte de un paciente. Por ello, en el Hospital Virgen del Rocío, centramos nuestro esfuerzo mediante la preparación diaria a través de sesiones clínicas y el esfuerzo personal. Se requiere un alto nivel de formación para prestar asistencia hospitalaria en una unidad como su propio nombre refleja: Servicio de cuidados críticos y urgencias.
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    Expression of certain proteins in the subfornical organ and cerebrospinal fluid of spontaneously hypertensive rats
    (Murcia: F. Hernández, 2007) Gonzalez-Marrero, I.; Carmona-Calero, E.; Fernández-Rodríguez, P.; Pérez-González, H.; Ormazabal-Ramos, C.; Castañeyra-Ruíz, L.; Perez-Garcia, C.G.; Martínez de la Peña y Valenzuela, Isabel; Castañeyra-Ruiz, A.; Castañeyra Perdomo, A.; Ferres Torres, R.
    The objective of this study was to analyze the proteins in the cerebrospinal fluid of spontaneously hypertensive rats and to study their possible role in the relationship between hydrocephalus, arterial hypertension and variations in the subfornical organ. Brains and cerebrospinal fluid from control Wistar- Kyoto rats and spontaneously hypertensive rats sacrificed with chloral hydrate were used. Cerebrospinal fluid and extract of subfornical organ were processed by protein electrophoresis. Antisera against protein bands of 141, 117 and 48 kDa and Concanavalin A were used for immunohistochemical and western blot study of the subfornical organ, adjacent circumventricular structures and cerebrospinal fluid. Ventricular dilation in the spontaneously hypertensive rats and the presence of quite a lot of protein bands in the cerebrospinal fluid of the hypertensive rats, which were either not observed or scarcely present in the cerebrospinal fluid of the Wistar- Kyoto rats, were confirmed. The subfornical organ, third ventricle ependyma and choroideus plexus showed immunoreactive material for antibodies against 141kDa, 117 and 48 kDa proteins band (anti-B1, anti-B2 and anti- B3). The larger amount of the immunoreactive material was found in the subfornical organ of the spontaneously hypertensive rat. Our results and the alterations observed by other authors in the subfornical organ in hydrocephalic and hypertensive rats support the possibility that this circumventricular organ, some proteins of the cerebrospinal fluid and ventricular dilation could be connected with the physiopathology of this type of hypertension.