Browsing by Subject "Cephenemyia stimulator"

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    Development and characterization of 15 novel polymorphic microsatellite loci for two important bot flies (Diptera, Oestridae) by next-generation sequencing
    (Springer, 2020) Aleix-Mata, Gaël; Expósito, Miriam; Ruiz Ruano, Francisco J.; López Beceiro, Ana María; Fidalgo, Luis E; Martínez Carrasco-Pleite, Carlos; Ruiz de Ybáñez Carnero, María del Rocío; Boos, Mathieu; Pérez, Jesús M.; Sánchez, Antonio; Sanidad Animal
    Cephenemyia stimulator and Oestrus ovis are two important parasitic bot flies (Oestridae) species causing myiasis, with a potential negative impact on the welfare of the host. Using next-generation sequencing approach and bioinformatics tools, a large panel of possible microsatellites loci was obtained in both species. Primer pairs were designed for 15 selected microsatellite loci in C. stimulator and other 15 loci in O. ovis for PCR amplification. Loci amplification and analysis were performed in four populations of each species. The results demonstrated that all selected loci were polymorphic, with the number of alleles ranging from 2 to 6 per locus in C. stimulator and 3 to 13 per locus in O. ovis. This is the first time to describe these microsatellite loci for C. stimulator and O. ovis. These two sets of microsatellite markers could be further used for biogeographic and population genetics studies.
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    Unexpected intracranial location of a Cephenemyia stimulator larva in a roe deer, Capreolus capreolus, revealed by computed tomography
    (Sociedad Española para la Conservación y Estudio de los Mamíferos - SECEM, 2021-02-14) Fidalgo Álvarez, Luis Eusebio; López-Beceiro, Ana M.; Martínez-Carrasco Pleite, Carlos; Caparrós-Fontarosa, Noelia; Sánchez Baca, Antonio; Vila, Mónica; Barreiro, Daniel; Sarasa, Mathieu; Pérez-Jiménez, Jesús M.; Sanidad Animal; Facultad de Veterinaria
    In this study we describe the finding of a Cephenemyia stimulator larva in the brain of a roe deer (Capreolus capreolus) after performing a computed tomography (CT) scan of its head. Despite this anatomical location of oestrid larvae could be relatively frequent in other genera, such as Oestrus, to our knowledge, this is the first reported case involving the genus Cephenemyia. Concretely, a second-instar C. stimulator larva was found in the basis of the cranium. The location of a macroscopic hemorrhagic lesion involving the brain parenchyma peripheral to the location of the larva suggests that tissue colonization occurred before the animal was hunted. Since no detectable alterations or damage to the cranial bones were observed, we suggest a possible larval migration route drilling the skull bones. Finally, we propose the use of the term “neuromyiasis” to be referred to the invasion of the central nervous system by dipteran larvae, particularly oestrids.
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    Use of Computed Tomography as a non-invasive method for diagnosing cephenemyiosis in roe deer (Capreolus capreolus)
    (Wiley, 2014-10-08) Fidalgo, Luis E.; López Beceiro, Ana M.; Vila Pastor, M.; Martínez-Carrasco Pleite, Carlos; Barreiro Vázquez, J. D.; Pérez Jiménez, Jesús M.; Sanidad Animal; Facultades de la UMU::Facultad de Veterinaria
    This study was conducted to assess the reliability of computed tomography (CT) for diagnosing bot fly infestations by Cephenemyia stimulator (Clark) (Diptera: Oestridae) in roe deer (Capreolus capreolus L.) (Artiodactyla: Cervidae). For this purpose, the heads of 30 animals were analysed, firstly by CT and then by necropsy, which was used as the reference standard method. The prevalence values obtained by both methods were identical; the prevalence of infestation was 40.0% overall, and was higher in males (45.5%) than in females (25.0%). These results highlight the usefulness of CT as an alternative or non-invasive method for diagnosing cephenemyiosis in live-captured roe deer and in hunting trophies or museum collections that cannot be destroyed or damaged.