Browsing by Subject "Cell functions"

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    MiR-194-3p modulates the progression of colorectal cancer by targeting KLK10
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2022) Liu, Tao; Fang, Yuejun
    Background. A rich history of studies have manifested the importance of miRNAs to cancer progression, while miR-194-3p has been seldom explored. Objective. The purpose of this study is to unearth the way the miR-194-3p/KLK10 axis modulates colorectal cancer (CRC). Methods. Differentially expressed genes of CRC in TCGA database were analyzed. Western blot and qRTPCR were employed to test protein and mRNA expressions of two researched genes. Their targeting was confirmed using dual-luciferase. Biological behaviors of cells were tested by a series of cellular functional assays. Result. Remarkably low miR-194-3p expression and high KLK10 expression were observed in cancer cells. Overexpressing miR-194-3p hindered the progression of CRC cells. Overexpression of miR-194-3p significantly weakened the promoting effect of upregulated KLK10 on cell migration, invasion and proliferation. Their targeting was verified by dual -luciferase assay. Therefore, miR-194-3p hindered cell behaviors of CRC through KLK10. Conclusion. This investigation casts new light on the treatment of CRC through the miR-194-3p/KLK10 axis.
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    MiR-5195-3p functions as a tumor suppressor by targeting RHBDD1 in ovarian cancer
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2023) Wang, Zhanyu; Zhang, Xiaoping; Liu, Yongying; Shi, Xiaoyan; Li, Lijun; Jia, Yun; Wu, Fangfang; Cui, Haosen; Li, Liang
    Background. Recent studies have reported the tumor suppressive role of miR-5195-3p in the progression of several cancers, but the potential roles of miR-5195-3p in ovarian cancer (OC) remain largely unknown. Methods. We first analyzed the expression levels of miR-5195-3p in 83 pairs of human OC tissues and adjacent specimens by reverse transcription-quantitative PCR. The correlation of miR-5195-3p/rhomboid domain containing 1 (RHBDD1) and clinicopathological parameters was analyzed by chi-square test. The prognostic value of miR-5195-3p was evaluated by Kaplan-Meier method Cox proportional hazards models. The effects of miR-5195-3p on cell proliferation, cell cycle distribution, migration and invasion were examined by CCK-8 assay, colony formation assay, flow cytometry and transwell assay. Tumor forming was evaluated by nude mice model in vivo. The association between miR-5195-3p and RHBDD1 was verified by luciferase reporter assay. Results. We observed that miR-5195-3p level was remarkably reduced in OC tissues as compared to adjacent tissues. The expression of miR-5195-3p was associated with FIGO stage, depth of invasion and poor survival prognosis in OC patients. Overexpression of miR-5195-3p significantly suppressed cell proliferation, cell cycle G1/S transition, migration and invasion in OC cell lines (SKOV-3 and OVCAR3), while knockdown of miR-5195-3p obtained the opposite results. We further confirmed miR-5195-3p as a negative posttranscriptional modulator of RHBDD1. RHBDD1 expression was upregulated in OC tissues compared with adjacent tissues, which was inversely correlated with miR-5195-3p expression. The expression of RHBDD1 was associated with FIGO stage and distant metastasis. RHBDD1 overexpression reversed the suppressive role of miR-5195-3p on OC cell proliferation, migration and invasion. Consistent with the in vitro results, miR-51953p overexpression decreased the growth of subcutaneously inoculated tumors in nude mice. Conclusions. Taken together, the present results indicated that miR-5195-3p acts a tumor suppressor by targeting RHBDD1 in OC.