Browsing by Subject "Capacitation"

Now showing 1 - 5 of 5
  • Thumbnail Image
    Publication
    Open Access
    Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
    (Frontiers Media, 2021-09-01) Delgado-Bermúdez, Ariadna; Recuero, Sandra; Llavanera, Marc; Mateo-Otero, Yentel; Sandu, Andra; Barranco Cascales, Isabel; Ribas-Maynou, Jordi; Yeste, Marc; Medicina y Cirugía Animal; Facultad de Veterinaria
    Aquaporins are a family of ubiquitous transmembrane proteins that allow the transport of water and small molecules across the cell plasma membrane. The different members of this family present a characteristic distribution across different cell types, which is species-specific. In mammalian sperm, different AQPs, including AQP3, AQP7, and AQP11, have been identified; their main roles are related to osmoadaptation and sperm motility activation after ejaculation. Capacitation, which is a post-ejaculatory process that sperm must undergo to achieve fertilizing ability, is triggered by pH changes and different extracellular ions that are present in the female reproductive tract. Considering the function of AQPs and their influence on pH through the regulation of water flow, this study aimed to elucidate the potential role of different AQPs during in vitro sperm capacitation using three different transition metal compounds as AQP inhibitors. Cooper sulfate, a specific inhibitor of AQP3, caused a drastic increase in peroxide intracellular levels compared to the control. Mercury chloride, an unspecific inhibitor of all AQPs except AQP7 produced an increase in membrane lipid disorder and led to a decrease in sperm motility and kinetics parameters. Finally, the addition of silver sulfadiazine, an unspecific inhibitor of all AQPs, generated the same effects than mercury chloride, decreased the intracellular pH and altered tyrosine phosphorylation levels after the induction of the acrosome reaction. In the light of the aforementioned, (a) the permeability of AQP3 to peroxides does not seem to be crucial for sperm capacitation and acrosome reaction; (b) AQPs have a key role in preserving sperm motility during that process; and (c) AQPs as a whole seem to contribute to the maintenance of lipid membrane architecture during capacitation and may be related to the intracellular signaling pathways involved in the acrosome reaction. Hence, further research aimed to elucidate the mechanisms underlying the involvement of AQPs in mammalian sperm capacitation and acrosome reaction is warranted.
  • Thumbnail Image
    Publication
    Open Access
    Epididymal and ejaculated sperm functionality is regulated differently by periovulatory oviductal fluid in pigs
    (Wiley, 2020-09-13) Soriano-Úbeda, Cristina; Avilés-López, Karen; García Vázquez, Francisco Alberto; Romero Aguirregomezcorta, Jon; Matas Parra, Carmen; Anatomía y Anatomía Patológica Comparada; Facultad de Veterinaria
    Background: The current results of in vitro reproduction techniques in pigs, such as in vitro fertilization (IVF) and embryo development, show high performance with both epididymal and ejaculated spermatozoa. However, the results using ejaculated spermatozoa are even better. Ejaculated spermatozoa are exposed to the secretions of the accessory seminal glands: the seminal plasma (SP). It has been reported that exposure of spermatozoa to reproductive fluids, such as SP or periovulatory oviductal fluid (pOF), modulates sperm functionality both in vivo and in vitro. But whether or not this modulating effect of pOF depends on the origin of the spermatozoa being epididymal or ejaculated, is still unknown. Objectives: To determine and compare the effect of pOF on epididymal and ejaculated sperm functionality. Material and methods: The effects of incubating spermatozoa from the epididymis and ejaculate with pOF in capacitating conditions were investigated by analyzing sperm motility, phosphorylation of protein kinase A substrates and proteins in tyros ine (pPKAs and pTyr, respectively), the interaction of the spermatozoa with the oocyte in IVF and intracytoplasmic sperm injection (ICSI), and, finally, the spermatozoa chromatin condensation status.Results: the pOF modified events related to capacitation in epididymal spermatozoa by decreasing motility, pPKAs and pTyr. In the interaction with the oocyte after sperm capacitation, pOF regulated the epididymal and ejaculated spermatozoa differently. While pOF decreased the number of spermatozoa bound to the zona pellucida (Spz/ZP) and increased oocyte activation after ICSI with epididymal spermatozoa, with the ejaculated spermatozoa, it decreased the mean number penetrating each oocyte (Spz/O). Additionally, pOF significantly increased the nuclear decondensation of the epididymal spermatozoa after the fertilization of the oocyte.Conclusion: The modulation of sperm functionality by pOF is conditioned by the origin of the spermatozoa.
  • Thumbnail Image
    Publication
    Open Access
    Formación continuada. Herramienta para la capacitación
    (Murcia : Servicio de Publicaciones de la Universidad de Murcia, 2011) Marqués Andrés, Susana
    Se realizó una búsqueda bibliográfica sobre „Formación Continuada? como tema central, obteniéndose algunos resultados que ponen de manifiesto la necesidad de reflexionar sobre aspectos que se plantean en el presente documento, con la finalidad última de mejorar la calidad de la atención y conseguir la mayor satisfacción con los cuidados de enfermería de la persona enferma y de sus familiares. La Formación Continuada actualiza y mejora competencias, cuenta con objetivos y es medible. Pero requiere una planificación basada en la detección de necesidades. Para ello es fundamental responder cuatro preguntas: cómo estamos, qué necesitamos y queremos, hasta dónde queremos llegar. Y motivar a los participantes para la participación activa y la implicación en la misma. La formación continuada puede considerarse un proceso de cambio de conducta cuando los cambios se transfieren a la práctica clínica. Además es necesaria a corto plazo, aunque ha de proyectarse al futuro, desarrollar competencias para mejorar la calidad y conseguir la excelencia clínica.
  • Thumbnail Image
    Publication
    Open Access
    Relación entre los problemas de salud de los mayores dependientes y la formación de los cuidadores informales
    (Murcia: Servicio de Publicaciones de la Universidad de Murcia, 2013) Guerra Martín, María Dolores; Zambrano Domínguez, Encarnación María
    Objetivo: Estudiar la relación entre los problemas de salud de los mayores dependientes y la formación de los cuidadores informales. Material y métodos: Se presenta un estudio exploratorio, descriptivo, de corte transversal, seleccionando a 41 cuidadores mediante muestreo no probabilístico intencional. Se ha diseñado un cuestionario basado en la bibliografía existente y cuya validez de contenido ha sido consensuada por expertos. Para el análisis de datos se ha utilizado SPSS versión 18.0. Resultados: Un 41,5% de los cuidadores informales recibieron algún tipo de formación; un 92,7% de cuidadores plantean que necesitan formación en relación con los problemas de salud de las personas cuidadas y un 58,5% de mayores han sido cuidadas por cuidadores que no han recibido formación. Conclusiones: Es significativo, que en nuestro estudio hay más cuidadores sin formación cuidando personas con problemas de salud, que con formación específica; siendo necesario que los cuidadores informales principales puedan asistir a los programas formativos sobre los problemas de salud y sobre los cuidados que requieran las personas mayores que cuidan, por lo que se plantea como estrategia de mejora, que desde la gestión sanitaria se proporcionen los recursos y medios que necesiten los cuidadores, y se potencie el uso de herramientas formativas (foros, chats, correos electrónicos, tuenti, facebook, blog, twitter, Skype, entre otros), que pueden plantearse como alternativa a las sesiones presenciales.
  • Thumbnail Image
    Publication
    Restricted
    Sperm functionality is differentially regulated by porcine oviductal extracellular vesicles from the distinct phases of the estrous cycle
    (CSIRO Publishing, 2024-05-07) Toledo-Guardiola, S.M.; Martínez Díaz, Pablo; Martínez-Núñez, R.; Navarro-Serna, S.; Soriano-Úbeda, C.; Romero Aguirregomezcorta, Jon; Matas Parra, Carmen; Anatomía y Anatomía Patológica Comparada; Facultad de Veterinaria
    Context: Extracellular vesicles (EVs) derived from the oviductal fluid (oEVs) play a critical role in various reproductive processes, including sperm capacitation, fertilisation, and early embryo development. Aims: To characterise porcine oEVs (poEVs) from different stages of the estrous cycle (late follicular, LF; early luteal, EL; mid luteal, ML; late luteal, LL) and investigate their impact on sperm functionality. Methods: poEVs were isolated, characterised, and labelled to assess their binding to boar spermatozoa. The effects of poEVs on sperm motility, viability, acrosomal status, protein kinase A phosphorylation (pPKAs), tyrosine phosphorylation (Tyr-P), and in in vitro fertility were analysed. Key results: poEVs were observed as round or cup-shaped membrane-surrounded vesicles. Statistical analysis showed that poEVs did not significantly differ in size, quantity, or protein concentration among phases of the estrous cycle. However, LF poEVs demonstrated a higher affinity for binding to sperm. Treatment with EL, ML, and LL poEVs resulted in a decrease in sperm progressive motility and total motility. Moreover, pPKA levels were reduced in presence of LF, EL, and ML poEVs, while Tyr-P levels did not differ between groups. LF poEVs also reduced sperm penetration rate and the number of spermatozoa per penetrated oocyte (P < 0.05). Conclusions: poEVs from different stages of the estrous cycle play a modulatory role in sperm functionality by interacting with spermatozoa, affecting motility and capacitation, and participating in sperm–oocyte interaction. Implications: The differential effects of LF and LL poEVs suggest the potential use of poEVs as additives in IVF systems to regulate sperm–oocyte interaction.