Browsing by Subject "CD28"

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    CD28 and KIR2D receptors as sensors of the immune status in heart and liver transplantation
    (Elsevier, 2011-06-22) Blanco García, R. M.; López Álvarez, María R.; Garrido, I. P.; Salgado Cecilia, G.; Campillo, José Antonio; Bolarín, José Miguel; Muro, Manuel; García Alonso, A.M.; Martínez Sánchez, María V.; Peña Moral, J.M. de la; Pascual Figal, Domingo A.; Álvarez López, María Rocio; Miras, M.; Minguela, Alfredo; Legaz Pérez, Isabel; Ciencias Sociosanitarias
    Viral infections and cellular acute rejection (AR) condition immunosuppressive therapy and compromise the evolution of allografts. Immune monitoring can be useful for ascertaining rejection and for differentiating allo-reaction from activation induced by infections. This work analyzes the usefulness of monitoring the expression of CD28 and KIR2D receptors in peripheral blood T lymphocytes by flow cytometry, to ascertain the immune response in heart and liver transplant recipients. In both types of transplant, the up-regulation of CD28 in CD4+ lymphocytes in the periods of greatest AR frequency indicates an effective allo-response, whereas the post-transplantation emergence of circulating CD8+CD28− and CD8+CD28−KIR2D+ T cells correlates with better early clinical results. Cytomegalovirus (CMV) infection, but not hepatitis C virus (HCV) or other infections, abrogated both CD28 up-regulation and CD8+CD28−KIR2D+ T-cell expansion. Our results show that monitoring the expression of CD28 and KIR2D receptors on T lymphocytes might be considered as sensors of the immune status of heart and liver recipients.
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    CD28 biomarker quantification and expression level profiles in CD4+ T-lymphocytes in solid organ transplantation
    (Elsevier, 2017-06) Boix, Francisco; Bolarín, José Miguel; Mrowiec, Anna; Eguía, Jorge; González Martínez, Gema; Peña, Jesús de la; Galían, José A.; Alfaro, Rafael; Moya Quiles, María R.; Campillo, José Antonio; Ramírez, Pablo; García Alonso, Ana; Pons, José A.; Sánchez Bueno, Francisco; Minguela, Alfredo; Llorente, Santiago; Muro, Manuel; Legaz Pérez, Isabel; Ciencias Sociosanitarias
    The introduction of anti-calcineurin-based therapies has led to an increase in the one-year survival as well as graft function rates in patients undergoing solid organ transplantation (SOT). Nonetheless, early cellular acute rejection (EAR) incidence still remains a major challenge that irrevocably heads to poor outcomes. The mechanisms underlying CD4 T cell activation in SOT are still under research. In this sense, CD28 co-stimulatory molecule plays a pivotal role triggering CD4 T cell activation as well as survival maintenance. Previous own studies stated the role that CD4+ CD28+ circulating T lymphocytes plays before and during EAR episodes. We assessed the percentage as well as the absolute number of CD28 molecules on CD4+ T cells as predictive surrogate biomarker of EAR in a prospective cohort of liver and kidney transplant recipients. Quantitative analysis of CD28 was carried out on whole peripheral blood samples by flow cytometry. Decreased pre-transplant expression of CD28 was associated with EAR in both study groups. Furthermore, the expression of CD28 within the rejected group, experimented an up-regulation upon transplantation. These preliminary results suggest that patients undergoing liver or kidney transplant can be stratified at high risk of EAR according to their CD28 molecule expression on peripheral CD4+ T lymphocytes.