Browsing by Subject "CD1a"

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    Dendritic and lymphocytic cell infiltration in prostate carcinoma
    (F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología, 2013) Yishan, Liu; Thorstein, Sæter; Vlatkovic, Ljiljana; Servoll, Einar; Waaler, Gudmund; Axcrona, Ulrika; Giercksky, Karl- Erik; Nesland, Jahn M.; Zhen-He, Suo; Axcrona, Karol
    We examined the distribution of CD1a+ cells and CD8+ and CD4+ T lymphocytes in prostate cancer (PCa) and correlated these with clinicopathological parameters. We also investigated whether the distribution of these cells was related to the expression of the cell membrane protein B7-H3, a putative negative regulator of the immune response expressed on PCa cells. A cohort of 151 PCa patients treated with radical prostatectomy (RP) was followed prospectively from 1985 until 2006 with a median follow-up of 9 years. Whole-mount sections of PCa specimens were immunostained to identify immune cells. A low number of CD1a+ cells was significantly associated with a high Gleason score and high pathological stage of pT3. The number of CD1a+ cells correlated significantly with the number of intratumoral and stromal CD8+ and stromal CD4+ lymphocytes. Kaplan-Meier analysis showed a tendency toward impaired biochemical progression-free survival in patients with few CD1a+ cells within their RP specimens. The expression of B7-H3 correlated inversely with the number of CD1a+ cells and intratumoral CD4+ lymphocytes; there was a trend for a similar inverse relationship between B7-H3 expression and the number of CD8+ lymphocytes. Our findings suggest that high-grade prostate carcinoma cells manipulate the immune system and that these changes contribute to the mechanism underlying tumor escape from immune surveillance.
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    Diagnostic usefulness of immunohistochemical evaluation of CD1a antigen and polyclonal antiLeishmania antibodies in cutaneous Leishmaniasis
    (Universidad de Murcia, Departamento de Biologia Celular e Histiologia, 2021) Lopez-Trujillo, Emilio; Gonzàlez-Farré, Mònica; Pujol, Ramon M.; Bellosillo, Beatriz; Fisa, Roser; Riera, Cristina; Alcover, Magdalena; Barranco, Carlos; Martin-Ezquerra, Gemma
    Background. Different immunohistochemical markers to detect amastigotes in cutaneous Leishmaniasis have been proposed with variable diagnostic usefulness. Objectives. To evaluate the diagnostic usefulness of immunohistochemical amastigotes identification by specific polyclonal anti-Leishmania antibodies and CD1a expression (clone EP3622) in a series of PCR confirmed cutaneous Leishmaniasis. Materials and methods. Thirty-three skin samples corresponding to PCR confirmed cutaneous Leishmaniasis were included in the study. All samples were stained with Hematoxylin-eosin and Giemsa. Moreover, immunohistochemical studies with anti-CD1a and anti-Leishmania antibodies were performed. The patients clinical features and the observed histopathological features were also recorded. Results. From the selected 33 biopsies, Leishmania spp. amastigotes were detected in 48.4% of cases with conventional Hematoxylin-eosin stain and in 57.5% of cases by Giemsa staining. In 31/33 cases, anti-CD1a allowed us to identify parasitic structures, and in 33/33 cases amastigotes were detected with anti-Leishmania antibodies. Concordance between both techniques, antiCD1a and anti-Leishmania, was 94% [CI 95%: (79,8%- 99,3%)]; p value <0.05. The sensitivity of anti-CD1a in comparison with the PCR was 94%, with a positive predictive value of 100%. Two cases of low parasitic index were negative for CD1a immunostaining. In cases with high parasitic index, anti-CD1a stained amastigotes in superficial and deep dermis. Only a few cases were originally diagnosed with the available histological techniques, needing PCR for Leishmania spp. identification. Conclusions. Anti-CD1a antibody seems to be a useful technique to identify amastigotes when PCR and anti-Leishmania antibodies are not available. The sensitivity to detect amastigotes is increased when the CD1a immunostaining is added to the classical Haematoxylin – eosin and Giemsa staining.