Browsing by Subject "Brassicaceae"

Now showing 1 - 4 of 4
  • Thumbnail Image
    Publication
    Open Access
    Biotic Elicitors Effectively Increase The Glucosinolates Content In Brassicaceae Sprouts
    (American Chemical Society, 2014-01-31) Baenas, Nieves; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    Several biotic elicitors have been used in Brassicaceae species to enhance their phytochemical quality. However, there is no comparison between elicitors under controlled-growth conditions. In order to draw general conclusions about the use of elicitors to enrich ready-to-eat sprouts in health-promoting glucosinolates, the aim of this study was to unvail the effect of the phytohormones methyl jasmonate (25µM), jasmonic acid (150 µM), and salicylic acid (100 µM), the oligosaccharides glucose (277mM) and sucrose (146mM), and the amino acid DL-methionine (5mM), as elicitors over 8-day sprouting Brassica oleraceae (broccoli), Brassica napus (rutabaga cabbage), Brassica rapa (turnip) and Raphanus sativus (China rose radish and red radish), representative species high in glucosinolates previously studied. Results indicated that the phytohormones methyl jasmonate and jasmonic acid, and the sugars, acted as effective elicitors, increasing the total glucosinolate contents and, particularly, health related compounds such as glucoraphanin, glucoraphenin, dehydroerucin and indoles, in all the Brassicaceae species studied.
  • Thumbnail Image
    Publication
    Open Access
    Broccoli and Radish Sprouts are Safe and Rich in Bioactive Phytochemicals
    (Elsevier, ) Baenas, Nieves; Gómez-Jodar, Isabel; Moreno, Diego A.; García-Viguera, Cristina; Periago, Paula M.; Tecnología de Alimentos, Nutrición y Bromatología
    Cruciferous sprouts (e.g. broccoli and red radish) are rich source of health-promoting phytochemicals more concentrated than the adult plant edible organs; however, these tiny microgreens need cold storage conditions to preserve their quality to reach the consumers in microbiologically safe conditions, maintaining their composition and acceptability. In this work, the microbiological status and phytochemical composition of broccoli and radish sprouts were evaluated at harvest (Day 0), and after seven and fourteen days of storage at two different temperatures, 5 and 10 °C. Pathogenic microorganisms were absent during shelf-life; nevertheless, the slight growth of Enterobacteriaceae organisms, aerobic mesophilic and psychrotrophic bacteria, molds and yeasts was assessed. The temperature of storage highly influenced the quality and content of bioactives in the sprouts, and for practical applications, the storage at 5 ºC is the most suitable option. Moreover, these fresh crucifers remain acceptable for consumers after a long refrigerated storage period (14 d), being an interesting option for consuming fresh and naturally-functional foods.
  • Thumbnail Image
    Publication
    Open Access
    Metabolic Activity of Radish Sprouts Derived Isothiocyanates in Drosophila melanogaster.
    (MDPI, 2016-02-18) Baenas, Nieves; Piegholdt, Stefanie; Schloesser, Anke; Moreno, Diego A.; García-Viguera, Cristina; Rimbach, Gerald; Wagner, Anika E.; Tecnología de Alimentos, Nutrición y Bromatología
    We used Drosophila melanogaster as a model system to study the absorption, metabolism and potential health benefits of plant bioactives derived from radish sprouts (Raphanus sativus cv. Rambo), a Brassicaceae species rich in glucosinolates and other phytochemicals. Flies were subjected to a diet supplemented with lyophilized radish sprouts (10.6 g/L) for 10 days, containing high amounts of glucoraphenin and glucoraphasatin, which can be hydrolyzed by myrosinase to the isothiocyanates sulforaphene and raphasatin, respectively. We demonstrate that Drosophila melanogaster takes up and metabolizes isothiocyanates from radish sprouts through the detection of the metabolite sulforaphane-cysteine in fly homogenates. Moreover, we report a decrease in the glucose content of flies, an upregulation of spargel expression, the Drosophila homolog of the mammalian PPARγ-coactivator 1 α, as well as the inhibition of α-amylase and α-glucosidase in vitro. Overall, we show that the consumption of radish sprouts affects energy metabolism in Drosophila melanogaster which is reflected by lower glucose levels and an increased expression of spargel, a central player in mitochondrial biogenesis. These processes are often affected in chronic diseases associated with aging, including type II diabetes mellitus
  • Thumbnail Image
    Publication
    Open Access
    Radish sprouts : Characterization and elicitation of novel varieties rich in anthocyanins
    (Elsevier, 2015-01-16) Baenas, Nieves; Ferreres, Federico; García-Viguera, Cristina; Moreno, Diego A.; Tecnología de Alimentos, Nutrición y Bromatología
    The anthocyanin profile of two varieties of red radish sprouts (Raphanus sativus), cv. China rose and Rambo, were studied using HPLC-DAD-ESI-MSn and HPLC-DAD. The most abundant type of anthocyanins was cyanidin and its derivatives, with one or two acylated groups, with qualitative and quantitative differences among varieties. Some compounds were identified for the first time in both varieties, as we are concern. Radish sprouts were treated during germination (day 3 to 8) using methyl jasmonate, jasmonic acid, salicylic acid, sucrose and glucose as elicitors in order to enrich their total anthocyanins content (TAC). An increase in TAC was achieved by 50% in China rose radish sprouts and by 30% in Rambo red radish after glucose treatment. Methyl jasmonate and sucrose also contribute to enhance TAC. Enriching natural food in anthocyanins may contribute to sustain their regular intake with preventive and therapeutic roles in a number of human diseases.