Browsing by Subject "Biodentine"

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    Open Access
    Biological properties of Ceraputty as a retrograde filling material: an in vitro study on hPDLSCs
    (Springer, 2023-05-01) López Garcia, Sergio; Rodríguez Lozano, Francisco Javier; Sanz, José Luis; Forner, Leopoldo; Pecci Lloret, María Pilar; Lozano, Adrián; Murcia, Laura; Sánchez Bautista, Sonia; Oñate Sánchez, Ricardo Elías; Dermatología, Estomatología, Radiología y Medicina Física
    Objectives To assess the cytocompatibility and bioactive potential of the new calcium silicate-based cement Ceraputty on human periodontal ligament stem cells (hPDLSCs) compared to Biodentine and Endosequence BC root repair material (ERRM). Materials and methods hPDLSCs were isolated from extracted third molars from healthy donors. Standardized sample discs and 1:1, 1:2, and 1:4 eluates of the tested materials were prepared. The following assays were performed: surface element distribution via SEM–EDX, cell attachment and morphology via SEM, cell viability via a MTT assay, osteo/cemento/odontogenic marker expression via RT-qPCR, and cell calcified nodule formation via Alizarin Red S staining. hPDLSCs cultured in unconditioned or osteogenic media were used as negative and positive control groups, respectively. Statistical analysis was performed using one-way ANOVA or two-way ANOVA and Tukey’s post hoc test. Statistical significance was established at p < 0.05. Results The highest Ca2+peak was detected from Biodentine samples, followed by ERRM and Ceraputty. hPDLSC viability was significantly reduced in Ceraputty samples (p < 0.001), while 1:2 and 1:4 Biodentine and ERRM samples similar results to that of the negative control (p > 0.05). Biodentine and ERRM exhibited an upregulation of at least one cemento/odonto/osteogenic marker compared to the negative and positive control groups. Cells cultured with Biodentine produced a significantly higher calcified nodule formation than ERRM and Ceraputty (p < 0.001), which were also higher than the control groups (p < 0.001). Conclusion Ceraputty evidenced a reduced cytocompatibility towards hPDLSCs on its lowest dilutions compared to the other tested cements and the control group. Biodentine and ERRM promoted a significantly higher mineralization and osteo/cementogenic marker expression on hPDLSCs compared with Ceraputty. Further studies are necessary to verify the biological properties of this new material and its adequacy as a retrograde filling material.
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    Open Access
    Effect of N-Hexyl Cyanoacrylate, Calcium Hydroxide Plus Iodoform and Two Calcium-Silicate-Based Restorative Cements on Pulp Repair
    (OMICS international, 2018-09-28) Pérez Silva, Amparo; Serna Muñoz, Clara; Casimiro de Andrade, JD.; Pinhao Ferreira, A.; Ortiz Ruiz, Antonio José; Dermatología, Estomatología, Radiología y Medicina Física
    Objective: The objective of this study was to evaluate the histological response of rat molars to four materials placed on the dental pulp. Methods: We used 24 female Wistar rats (6 per group). The pulp chamber roof of the first and second upper molars was perforated. After control of bleeding (<5 minutes), a pulp coating material (ProRoot®MTA, IfabondTM, Calcipast+I or BiodentineTM) was placed. After 30 days of treatment, the molars were observed under an optical microscope. Results: ProRoot®MTA produced no inflammation of any pulp (p<0.0005). With Calcipast+I, inflammation was absent only in 18.7% of the pulps. All samples in all groups presented pulpal vitality. The ProRoot®MTA, IfabondTM and BiodentineTM groups showed a dentin bridge and reparative dentin in all pulps. Only ProRoot®MTA showed a completely regular odontoblastic layer (p<0.0005). IfabondTM and BiodentineTM generated an irregular odontoblastic layer in all pulps (p<0.00l) and Calcipast+I showed 50% regular and 50% irregular layers. All samples treated with ProRoot®MTA (p<0.0005), 81.25% with Calcipast+I and only 37.5% of those treated with IfabondTM and BiodentineTM (p<0.0005) showed fibrosis. In all groups, calcifications appeared in areas outside the dentin bridge (p=0.027). Conclusion: Although ProRoot®MTA had the best performance, the response of the pulp of rat molars to the materials used was similar according to the histological criteria of degree of inflammation and pulpal vitality. The regularity or irregularity of the odontoblastic layer did not influence the presence of a dentin bridge.