Browsing by Subject "Biochemical profile"

Now showing 1 - 4 of 4
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    In vivo evaluation of the protein quality of lyophilized egg white as substitute for casein in elaboration of an experimental diet based on AIN93
    (Universidad de Murcia. Servicio de publicaciones, 2020) Ferreira-Chaves, Wenicios; Lins Pinheiro, Isabeli; Cadete Lucena-Cavalcanti, Carolina; do Nascimento Barreto, Alvaro; da Silva, Breno José; Manhães de Castro, Raul; da Silva Aragão, Raquel
    El objetivo del estudio fue evaluar in vivo la calidad de la proteína de la clara de huevo liofilizada como sustituto de la caseína en la dieta AIN93. Se separaron ratones Wistar machos (n= 30) según las dietas: caseína (CS), clara de huevo (EW) y sin proteína (PF). Se evaluó el crecimiento somático y la ingesta de alimentos. Se midieron la relación de eficiencia de proteína, la relación neto de proteína, el perfil bioquímico en suero, los pesos de los órganos y de los tejidos. La relación de eficacia de proteína, muestra que EW presenta un alto valor biológico. Sin embargo, EW mostró un crecimiento menor que el CS. El peso relativo de los órganos y la creatinina sérica se elevaron en el grupo EW. Por lo tanto, EW promueve la reducción del crecimiento, a pesar de la mayor ingesta
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    Long term study of the blood plasma biochemical profile of cattle born by assisted reproductive technologies
    (Brazilian College of Animal Reproduction, 2024) Serrano-Albal, Maria; Canovas, Sebastian; Heras, Sonia; Quintero-Moreno, Armando Arturo; Lopes, Jordana Sena; Soriano-Ubeda, Cristina; Gadea, Joaquin; Coy, Pilar; Romar, Raquel; Romero Aguirregomezcorta, Jon; Fisiología
    Assisted reproductive technology (ART) is key in cattle breeding as it enables selective mating and improves both reproductive efficiency and genetic improvement. However, there is still limited information on the long-term effects of these methods on the health and development of the offspring. This study aims to investigate the biochemical profile of cattle born through ART, from a previous study (Lopes et al., 2020), over an extended period to understand how these techniques may affect their health and metabolism. Male and female (not pregnant or lactating) cattle born after artificial insemination (AI, N= 7) were compared to those derived from in vitro produced embryos (IVP) using a standard protocol (C-IVP, N= 7), or using oviductal and uterine fluids in the process (RF-IVP, N= 4). Males and females were kept in two diferent open pens, but fed and managed under identical conditions their whole lives. Animals were studied every six months, from 1.5 years old until ≥4 years old, since their first year of life was evaluated by Lopes et al. (2022). Tail vein blood plasma was obtained by centrifugation (1000 G, 10 min) in lithium heparin tubes and stored at 80°C. The biochemical profile included total protein (TP), albumin (ALB), globulin (GLOB), creatinine (CREA), urea (URE), glucose (GLUC), cholesterol (CHOL), triglycerides (TRIG), amylase (AMIL), lipase (LIP), creatinine kinase (CK), alkaline phosphatase (ALP), gamma-glutamyl ranspeptidase (GGT), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin (TB). After serial dilutions, inter- and intraassay precision of the methods were lineal and below 15%. Data normal ly distributed was analysed using ANOVA and Tukey’s test; data not normally distributed was analysed using the Kruskal Wallis and Games Howell’s test (statistical significance p<0.05). The data is shown below as means by group over the years. The AI group had lower CREA and CHOL levels (1.43±0.05 mg/dL and 109.97±5.27 mg/dL, respectively) than C-IVP (1.75±0.07 mg/dL and 126.57±7.53 mg/dL, respectively) and RF-IVP (1.86±0.08 mg/dL and 140.01±7.19 mg/dL, respectively). The GGT levels were lower in RFIVP animals (13.00±1.30 UI/L) than both the C-IVP (17.55±1.10 UI/L) and AI groups (16.05±1.23 UI/L). Moreover, the levels of ALT were significantly lower in C-IVP (28.60±1.72 UI/L) than in the AI (34.35±1.81 UI/ L) and RF-IVP groups (33.40±2.24 UI/L). Depending on the age of the animal at the time of sample collection, significant differences were observed for most parameters. The parameters TP, GLOB, CREA, URE, AMIL, AST an increased in their concentration was observed as the animals aged, whilst ALB, GLUC, ALP decreased with age. However, all values can be considered within physiological ranges despite the significant v ariations. To conclude, the study showed differences in some biochemical parameters amongst the ART groups and with aging. However, despite the diferences all values were within physiological ranges. These findings are key to study the potential implications of this diferences on the animal’s health.
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    Long-term study of physical, haematological and biochemical parameters in cattle with different embryo origins
    (MDPI, 2025-06-14) Serrano-Albal, Maria; Canovas, Sebastian; Heras, Sonia; Gadea, Joaquin; Coy, Pilar; Romar, Raquel; Romero Aguirregomezcorta, Jon; Fisiología
    Assisted reproductive technologies are vital in cattle breeding to improve genetic selection and productivity. While early-life differences between artificially inseminated (AI) and in vitro-produced (IVP) cattle have been studied, long-term physiological, haematological, and biochemical effects remain unclear. This observational study assessed AI and IVP cattle from 1.5 to 5 years of age to determine if early differences persist. IVP cattle were produced after the transfer of the embryo produced by supplementing (RF-IVP group) or not supplementing (C-IVP) the embryo culture with oviductal and uterine fluids. Physical evaluations showed body mass index increased until 3.5 years, while temperature and respiratory rate declined with age, with no significant differences between AI and IVP groups. Haematological analysis revealed age-related changes, including decreased red and white blood cell counts and increased mean corpuscular volume and haemoglobin. AI cattle had higher white blood cell counts than IVP groups. Sex significantly influenced many haematological variables. Biochemical analysis showed age-related increases in total protein, creatinine, and urea, and decreases in glucose and alkaline phosphatase. AI cattle had lower cholesterol and creatinine than IVP groups. Despite group differences, all values remained within normal ranges. Sex affected albumin, cholesterol, triglycerides, and creatine kinase. This study provides the first long-term haematological and biochemical reference values for cattle from different reproductive methods, showing that age is the main influencing factor and supporting IVP cattle as a viable alternative to AI in breeding programs.
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    Open Access
    Long-term study of physical, haematological, and biochemical parameters in cattle with different embryo origins
    (MDPI, 2025-06-14) Serrano-Albal, María; Romero Aguirregomezcorta, Jon; Cánovas Bernabé, Sebastián; Heras García, Sonia; Gadea Mateos, Joaquín; Coy Fuster, Pilar; Romar Andrés, Raquel; Anatomía y Anatomía Patológica Comparada; Facultad de Veterinaria
    Assisted reproductive technologies are vital in cattle breeding to improve genetic selection and productivity. While early-life differences between artificially inseminated (AI) and in vitro-produced (IVP) cattle have been studied, long-term physiological, haematological, and biochemical effects remain unclear. This observational study assessed AI and IVP cattle from 1.5 to 5 years of age to determine if early differences persist. IVP cattle were produced after the transfer of the embryo produced by supplementing (RF-IVP group) or not supplementing (C-IVP) the embryo culture with oviductal and uterine fluids. Physical evaluations showed body mass index increased until 3.5 years, while temperature and respiratory rate declined with age, with no significant differences between AI and IVP groups. Haematological analysis revealed age-related changes, including decreased red and white blood cell counts and increased mean corpuscular volume and haemoglobin. AI cattle had higher white blood cell counts than IVP groups. Sex significantly influenced many haematological variables. Biochemical analysis showed age-related increases in total protein, creatinine, and urea, and decreases in glucose and alkaline phosphatase. AI cattle had lower cholesterol and creatinine than IVP groups. Despite group differences, all values remained within normal ranges. Sex affected albumin, cholesterol, triglycerides, and creatine kinase. This study provides the first long-term haematological and biochemical reference values for cattle from different reproductive methods, showing that age is the main influencing factor and supporting IVP cattle as a viable alternative to AI in breeding programs.