Browsing by Subject "Bactericidal activity"

Now showing 1 - 4 of 4
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    Evaluation of immune-related parameters in acellular and cellular fractions in male and female of Atlantic blue crabs (Callinectes sapidus)
    (Elsevier, 2025-05-25) Gálvez, Ricardo; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología; Facultad de Biología
    Brachyurans are relatively understudied compared to other decapods, with numerous fields remaining to be explored, including the immune response. Therefore, the aim of this study was to characterize several immune-related enzymes and activities of the Atlantic blue crab (Callinectes sapidus) in cell-free haemolymph (CFH) and in the haemocyte lysate supernatant (HLS), considering the gender factor and the suitability of measuring each activity in CFH or HLS. Data were expressed by millilitres of haemolymph or milligrams of protein to identify which of these units would be more robust and informative. For this, haemolymph of each gender was collected, and centrifuged to obtain CFH samples, whilst the cells were sonicated to get the HLS. Our results revealed that the protein concentration was higher in CFH compared to HLS. Considering milligrams of protein as units, phenoloxidase and esterase activities were lower in CFH regarding to HLS in both genders. Similarly, the lysozyme and phosphatase activities were lower in CFH compared to HLS but only in females. The values of lysozyme activity were higher in HLS of females compared to males. Studying millilitre of haemolymph as units, lysozyme activity exhibited higher activity in CFH compared to HLS in both genders, whilst phenoloxidase and esterase activities were higher in HLS, showing variations only in males. Interestingly, alkaline phosphatase activity was undetected in CFH male. Any variation was observed between the activities measured in both genders. Protease and antiprotease activities were higher and lower in CFH compared to HLS values, respectively, whilst any variation was observed between genders. Bactericidal activity against V. harveyi, V. alginolyticus and V. anguillarum exhibited stronger values in HLS than in CFH, independently of gender, as well as against P. damselae in females. Considering gender factor, the bactericidal activity against V. alginolyticus, V. anguillarum and P. damselae was higher in HLS of females than in males. However, the bactericidal activity against V. alginolyticus and P. damselae was higher in CFH of males compared to females. Our results could contribute to gender selection in future brachyurans studies considering the variability between males and females due to their distinct biology. Additionally, we provide data regarding the baseline values of several immune-related activities, establishing the most appropriate units from the biological point of view.
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    Evaluation of Stryphnodendron polyphyllum (Mart.) pollen as antioxidant and antibacterial potential
    (Universidad de Murcia. Servicio de publicaciones, 2023) Nogueira Oliveira Albuquerque, Rafael; Gomes Martins, Carlos Henrique; de Souza, Jonathan Henrique; de Melo Sacramento, Verônica; Alves de Oliveira, Dario; Farias de Melo-Júnior, Afrânio; Veiga Menezes, Elytania; Fernandes Souza, Luciano; de Andrade Royo, Vanessa
    El polen de Stryphnodendron polyphyllum, conocido como barbatimão, es un problema para los apicultores con apiarios cerca de la planta, por la toxicidad para las abejas. El objetivo fue evaluar la actividad antioxidante, antibacteriana y determinar fenoles totales y taninos en el extracto etanólico. La actividad antioxidante se evaluó por método DPPH. La actividad antibacteriana por método de microdilución. Los taninos totales y fenoles se determinaron por método espectrofotométrico. Se identificó actividad inhibitoria y bactericida para E. coli, P. aeruginosa, S. mutans, S. sobrinus, S. sanguinis, S. salivarius y L. paracasei. Se determinó EC50 de 7.42- mg/mL en la actividad antioxidante. Para compuestos fenólicos y tánicos, por comparación con curva de calibración, 3,12±0,005g/ 100g de muestra como ácido gálico y 7,3±0,03g/100g como ácido tánico. Se realizó cromatografía líquida de alta resolución para obtener el perfil cromatográfico.
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    Role of mucosal immune response and histopathological study in European eel (Anguilla anguilla L.) intraperitoneal challenged by Vibrio anguillarum or Tenacibaculum soleae
    (Elsevier, 2021-05-18) Conforto, Enmanuelle; Vilchez Gómez, Luciano; Parrinello, Daniela; Giovanna,Parisi, María; Esteban Abad, María de los Ángeles; Cammarata, Matteo; Guardiola Abellán, Francisco Antonio; Biología Celular e Histología; Facultad de Biología
    The external mucus layer that covers fish skin contains numerous immune substances scarcely studied that act as the first line of defence against a broad spectrum of pathogens. This study aimed to characterize and describe for the first time several humoral immune defence parameters in the skin mucus of the European eel (Anguilla anguilla) after intraperitoneal injection with Vibrio anguillarum or Tenacibaculum soleae. This study evaluated several immune-related enzymes and bactericidal activity against fish pathogenic bacteria in the skin mucus of European eels at 24, 48, and 72 h post-challenge. The results demonstrated that European eel skin mucus showed significant increments in peroxidase and lysozyme activity at 48 and 72 h after V. anguillarum challenge, compared to other experimental groups. In the case of antiprotease activity, an increase was observed at 24 h in the skin mucus of fish challenged with V. anguillarum compared to unchallenged fish, while this activity was undetected at 48 and 72 h. In contrast, protease activity had decreased at 48 and 72 h in the skin mucus of fish challenged with V. anguillarum compared to the unchallenged group. Regarding bactericidal activity, a high growth capacity of T. soleae was observed in the skin mucus of all experimental groups. Interestingly, the skin mucus from fish challenged with V. anguillarum exhibited increased bactericidal activity against this bacterium at 48 h, compared to unchallenged fish. Finally, severe histopathological alterations were observed in the gills and liver at the end of the trial (72 h), whereas the skin showed only an overspread presence of goblet cells in the challenged fish compared to unchallenged fish. The present results may give new insights into the mucosal immune system of this primitive species with potential applications in aquaculture.
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    Terminal carbohydrates abundance, immune related enzymes, bactericidal activity and physico-chemical parameters of the Senegalese sole (Solea senegalensis, Kaup) skin mucus
    (Elsevier , 2016-11-09) Collado-González, Mar; Guardiola Abellán, Francisco Antonio; Cuartero, María; Díaz Baños, F. Guillermo; Cuesta Peñafiel, Alberto; Moriñigo, Miguel Angel; Esteban Abad, María de los Ángeles; Biología Celular e Histología
    Recently, interest in mucosal surfaces, more specifically fish skin and its secreted mucus, has greatly increased among immunologists. The abundance of terminal carbohydrates, several enzymes (proteases, lysozyme, peroxidase, alkaline phosphatase, esterases and ceruloplasmin), bactericidal activity against fish pathogenic and non-pathogenic bacteria and several physico-chemical parameters (protein concentration, pH, conductivity, redox potential, osmolarity, density and viscosity) in the skin mucus of Senegalese sole (Solea senegalensis, Kaup) have been evaluated. Present results evidence the abundance of N-acetylneuraminic acid, mannose, glucose and N-acetyl-galactosamine in skin mucus. The levels of lysozyme, proteases, esterases and alkaline phosphatase were very similar (from 20 to 30 Units mg−1 protein). However, 93 Units mg−1 protein were detected of ceruloplasmin and only 4′88 Units mg−1 protein of peroxidase. Skin mucus of S. senegalensis showed high bactericidal activity against the tested pathogen bacteria but weak activity against non-pathogenic bacteria. Finally, a clear relationship between mucus density and temperature was detected, while viscosity showed a direct shear- and temperature-dependent behaviour. These results could be useful for better understanding the role of the skin mucus as a key component of the innate immune system, as well as, for elucidating possible relationships between biological and physico-chemical parameters and disease susceptibility.